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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 54 issue 8 (august 2020) : 942-947,   Doi: 10.18805/ijar.B-3904

In-vivo Analgesic and In-vitro Cytoprotective Potential of Various Leaf Extracts of Bitter Apple

R
Rashmi Sagar
V
V.K. Dumka
D
Dipak Deka
1Department of Veterinary Pharmacology and Toxicology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.
Cite article:- Sagar Rashmi, Dumka V.K., Deka Dipak (2020). In-vivo Analgesic and In-vitro Cytoprotective Potential of Various Leaf Extracts of Bitter Apple. Indian Journal of Animal Research. 54(8): 942-947. doi: 10.18805/ijar.B-3904.

ABSTRACT

The present study investigated the potential of different leaf extracts of Bitter Apple (Citrullus colocynthis) as an analgesic agent and In-vitro cytoprotective ameliorative effects of the various extracts in thiomethoxam-induced toxicity in MDBK cell lines. Different leaf extracts of Citrullus colocynthis i.e. alcoholic, acetone and chloroform were investigated for analgesic activity at the dose rate of 50 mg/kg and 100 mg/kg in Wistar rats. For the assessment of analgesic activity, tail flick method was used. In-vitro cytoprotective activity of various leaf extracts (at concentrations of 5% and 10%) was evaluated in ATCC acquired MDBK cell lines and for this study, cytotoxicity was induced by thiomethoxam. For cytoprotective study, oxidative stress parameters- catalase, LPO, SOD and GPx were determined. Study on analgesic activity revealed the presence of dose dependent effect in all extracts with highest effect in alcoholic extract of Bitter Apple. It is believe that triterpene alkaloids and steroidal principles present in the plant products might be responsible for the analgesic effect. 

REFERENCES

  1. Aba, P.E. and Asuzu I.E. (2019).Effect of administration of methanol root bark extract of Cussonia arborea on serum lipid profile and oxidative biomarker parameters in alloxan-    induced diabetic rats.Indian Journal of Animal Research. 53:1006-1013.
  2. Ahmad, I., Mehmood, Z. and Mohammad, F. (1998).Screening of some Indian medicinal plants for their antimicrobial properties. Journal of Ethnopharmacology. 62: 183-193.
  3. Bergmeyer, H. U. (1983). UV method of catalase assay. Methods of Enzymatic Analysis 3.
  4. D’Amour, F.E. and Smith. D. L. (1941). A method for determining loss of pain sensation.Journal of Pharmacology and Experimental Therapeutics. 72: 74-79.
  5. Graham, J. G., Quinn, M. L., Fabricant, D. S and Farnsworth, N. R. (2000). Plant used against cancer - an extension of the work of Jonathan Hartwell. Journal of Ethnopharmacology. 73: 347-377.
  6. Hassan, S. W., Mshelia, P. Y., Abubakar, M. G., Adamu, Y. A. and Yakubu, A. S. (2015). Wound healing, antioxidants and toxicological properties of root extracts of Kigelia africana (Lam) Benth. International Journal of Sciences: Basic and Applied Research. 19: 251-268.
  7. Madesh, M. and Balasubramanian, K.A. (1998). Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J Biochem Biophys. 35:184–188.
  8. Ostovan, F. (2014). The Citrullus colocynthis pulp antioxidant activity on oxidative stress factors of liver in streptozotocin-induced diabetic rats. Physiol Pharmacol. 17(4): 388-398
  9. Rajamanickam, E., Gurudeeban, S., Ramanathan, T. and Satyavani, K. (2010). Evaluation of anti-inflammatory activity of Citrullus colocynthis.International Journal of Current Research. 2: 67-69.
  10. Ravishankar, B. and Shukla, V. J. (2007). Indian systems of medicine: a brief profile African Journal of Traditional, Complementary and Alternative Medicines. 4: 319-37.
  11. Sani, M. F., Kouhsari, S. M. and Moradabadi, L. (2012). Effects of three medicinal plants extracts in experimental diabetes: antioxidant enzymes activities and plasma lipids profiles in comparison with metformin. Iranian Journal of Pharmaceutical Research. 11: 897.
  12. Sparg, S. G., Van-Staden, J and Jäger, A. K. (2000).Efficiency of traditionally used South African plants against schisto- somiasis. Journal of Ethnopharmacology.73: 209-214.
  13. Surana, S. J., Tatiya, A. U., Jain, A.S., Desai, D.G., Shastri, K.V and Katariya.M .V. (2008). Pharmacognostical and physicochemical standardization of root of Eranthemumroseum (Vahl) RBr. Pharmacognosy Magazine. 4: 75-79.
  14. Tannin-Spitz, T., Grossman, S., Dovrat, S., Gottlieb, H. E. and Bergman, M. (2007). Growth inhibitory activity of cucur- bitacinglucosides isolated from Citrullus colocynthison human breast cancer cells. Biochemical Pharmacology. 73 (1): 56–67.
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    Research Article

    volume 54 issue 8 (august 2020) : 942-947,   Doi: 10.18805/ijar.B-3904

    In-vivo Analgesic and In-vitro Cytoprotective Potential of Various Leaf Extracts of Bitter Apple

    R
    Rashmi Sagar
    V
    V.K. Dumka
    D
    Dipak Deka
    1Department of Veterinary Pharmacology and Toxicology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.
    Cite article:- Sagar Rashmi, Dumka V.K., Deka Dipak (2020). In-vivo Analgesic and In-vitro Cytoprotective Potential of Various Leaf Extracts of Bitter Apple. Indian Journal of Animal Research. 54(8): 942-947. doi: 10.18805/ijar.B-3904.

    ABSTRACT

    The present study investigated the potential of different leaf extracts of Bitter Apple (Citrullus colocynthis) as an analgesic agent and In-vitro cytoprotective ameliorative effects of the various extracts in thiomethoxam-induced toxicity in MDBK cell lines. Different leaf extracts of Citrullus colocynthis i.e. alcoholic, acetone and chloroform were investigated for analgesic activity at the dose rate of 50 mg/kg and 100 mg/kg in Wistar rats. For the assessment of analgesic activity, tail flick method was used. In-vitro cytoprotective activity of various leaf extracts (at concentrations of 5% and 10%) was evaluated in ATCC acquired MDBK cell lines and for this study, cytotoxicity was induced by thiomethoxam. For cytoprotective study, oxidative stress parameters- catalase, LPO, SOD and GPx were determined. Study on analgesic activity revealed the presence of dose dependent effect in all extracts with highest effect in alcoholic extract of Bitter Apple. It is believe that triterpene alkaloids and steroidal principles present in the plant products might be responsible for the analgesic effect. 

    REFERENCES

    1. Aba, P.E. and Asuzu I.E. (2019).Effect of administration of methanol root bark extract of Cussonia arborea on serum lipid profile and oxidative biomarker parameters in alloxan-    induced diabetic rats.Indian Journal of Animal Research. 53:1006-1013.
    2. Ahmad, I., Mehmood, Z. and Mohammad, F. (1998).Screening of some Indian medicinal plants for their antimicrobial properties. Journal of Ethnopharmacology. 62: 183-193.
    3. Bergmeyer, H. U. (1983). UV method of catalase assay. Methods of Enzymatic Analysis 3.
    4. D’Amour, F.E. and Smith. D. L. (1941). A method for determining loss of pain sensation.Journal of Pharmacology and Experimental Therapeutics. 72: 74-79.
    5. Graham, J. G., Quinn, M. L., Fabricant, D. S and Farnsworth, N. R. (2000). Plant used against cancer - an extension of the work of Jonathan Hartwell. Journal of Ethnopharmacology. 73: 347-377.
    6. Hassan, S. W., Mshelia, P. Y., Abubakar, M. G., Adamu, Y. A. and Yakubu, A. S. (2015). Wound healing, antioxidants and toxicological properties of root extracts of Kigelia africana (Lam) Benth. International Journal of Sciences: Basic and Applied Research. 19: 251-268.
    7. Madesh, M. and Balasubramanian, K.A. (1998). Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J Biochem Biophys. 35:184–188.
    8. Ostovan, F. (2014). The Citrullus colocynthis pulp antioxidant activity on oxidative stress factors of liver in streptozotocin-induced diabetic rats. Physiol Pharmacol. 17(4): 388-398
    9. Rajamanickam, E., Gurudeeban, S., Ramanathan, T. and Satyavani, K. (2010). Evaluation of anti-inflammatory activity of Citrullus colocynthis.International Journal of Current Research. 2: 67-69.
    10. Ravishankar, B. and Shukla, V. J. (2007). Indian systems of medicine: a brief profile African Journal of Traditional, Complementary and Alternative Medicines. 4: 319-37.
    11. Sani, M. F., Kouhsari, S. M. and Moradabadi, L. (2012). Effects of three medicinal plants extracts in experimental diabetes: antioxidant enzymes activities and plasma lipids profiles in comparison with metformin. Iranian Journal of Pharmaceutical Research. 11: 897.
    12. Sparg, S. G., Van-Staden, J and Jäger, A. K. (2000).Efficiency of traditionally used South African plants against schisto- somiasis. Journal of Ethnopharmacology.73: 209-214.
    13. Surana, S. J., Tatiya, A. U., Jain, A.S., Desai, D.G., Shastri, K.V and Katariya.M .V. (2008). Pharmacognostical and physicochemical standardization of root of Eranthemumroseum (Vahl) RBr. Pharmacognosy Magazine. 4: 75-79.
    14. Tannin-Spitz, T., Grossman, S., Dovrat, S., Gottlieb, H. E. and Bergman, M. (2007). Growth inhibitory activity of cucur- bitacinglucosides isolated from Citrullus colocynthison human breast cancer cells. Biochemical Pharmacology. 73 (1): 56–67.
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