Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 11 (november 2020) : 1422-1427

Clinical and Radiographic Effects of Bone Marrow Derived Mesenchymal Stem Cells Along with Local Insulin Therapy in Healing of Segmental Bone Defect in Diabetic Rabbits

Sonu Jaiswal, Narendra Singh Jadon, Deepti Bodh, R.K. Vishwakarma
1Department of Surgery and Radiology, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture andTechnology, Pantnagar-263 145, Uttarakhand, India.
Cite article:- Jaiswal Sonu, Jadon Singh Narendra, Bodh Deepti, Vishwakarma R.K. (2019). Clinical and Radiographic Effects of Bone Marrow Derived Mesenchymal Stem Cells Along with Local Insulin Therapy in Healing of Segmental Bone Defect in Diabetic Rabbits. Indian Journal of Animal Research. 54(11): 1422-1427. doi: 10.18805/ijar.B-3900.

ABSTRACT

The objective of present study was to investigate the efficacy of cultured allogenic bone marrow derived mesenchymal stem cell (BMMSCs) implant with insulin therapy for increasing osteosynthesis in bone gap defects in diabetic rabbits. Thirty six, clinically healthy New Zealand White rabbits were divided into four groups: A, B, C and D. Diabetes was induced experimentally in all rabbits, except group A. A 5 mm bone defect was created in right radii of all rabbits and following treatment was given: hydroxyapatite granules (group A and B), hydroxyapatite granules and insulin therapy (group C), cultured allogenic BMMSCs in hydroxyapatite scaffold and insulin therapy (group D). Clinical and radiographic parameters were recorded at days 0, 30, 60 and 90. It may be conducted that cultured allogenic bone marrow derived mesenchymal stem cells in hydroxyapatite scaffold along with local insulin therapy produces faster and better healing in alloxan-induced diabetic rabbits compared to healthy control, untreated diabetic and local insulin treated groups.

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