Indian Journal Of Animal Research

Association Study of Polymorphic Varients of Beta (B) Casein Gene with Milk Production Traits (Lactose, Snf and Density) in Malvi, Nimari, Sahiwal and H.F. Cross Breeds Cow

DOI: 10.18805/IJAR.B-3896    | Article Id: B-3896 | Page : 1-5
Citation :- Association Study of Polymorphic Varients of Beta (B) Casein Gene with Milk Production Traits (Lactose, Snf and Density) in Malvi, Nimari, Sahiwal and H.F. Cross Breeds Cow.Indian Journal Of Animal Research.2021.(55):1-5
Akhilesh Pandey, M.S. Thakur, Y. Pandey akpandey1109@rediffmail.com
Address : Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India.
Submitted Date : 8-08-2019
Accepted Date : 21-07-2020
First Online:

Abstract

Background: The most frequently observed forms of beta casein (β-Cn) in dairy cattle are A1 and A2. A2 β-Cn is recognized as the original β-Cn protein because it existed before a mutation caused the appearance of A1 β-Cn in European cattle (Bos taurus) a few thousand years ago. In cattle, beta-casein (CSN2) gene is highly polymorphic with at least 13 genetic variants known until now.
Methods: Research work was carried out on 50 Malvi and 50 Nimari, 50 Sahiwal and 50 H F Cross bred cow. In present research work the PCR amplicons of 121bp were digested by restriction endonuclease enzyme DdeI, which recognizes G^AATTC sites. Present association study of polymorphic variants’ showed that the presence of no restriction sites for the enzyme DdeI in both Malvi and Nimari. So one band of 121bp was observed on the gel and such genotype was designated as A2A2 type. Whereas in Sahiwal and H F Crossbred showed two type of genotypes A1A2 and A2A2.
Result: The result of RFLP revealed that the gene and genotypic frequencies of β-casein (CSN2) gene for A2A2 was 1.00 for both Malvi and Nimari breed of cattle but 0.00, 0.30 and 0.70 in Sahiwal and 0.00, 0.64 and 0.36 in HF crossbred cattle, respectively. Association study showed that the lactose per cent was significantly higher in Nimari as compared to Malvi and Sahiwal. Among all the four breeds of cattle, significantly lower SNF (%) and density was noticed in Malvi breed of cattle for A2A2 genotype compared to remaining three breeds.

Keywords

Beta (β) Casein Gene H F Cross bred Malvi Nimari Polymorphism Sahiwal

References

  1. Bell, J.S., Gregory, T.G. and Andrew, J.C. (2006). Health implications of milk containing β-Casein with the A2 genetic variant. Food Sciences and Nutrition. 46: 93-100.
  2. Cardak, A.D. (2005). Effects of genetic variants in milk protein on yield and composition of milk from Holstein-Friesian and Simmentaler cows. South African Journal of Animal Science. 35 (1): 41-47.
  3. Farrel, H.M.R, Jimenez, F., Bleck, G.T., Brown, E.M., Butler, J.E. and Creamer, L.K. (2004). Nomenclature of the proteins of cows’ milk - sixth revision. Journal of Dairy Sciences, 87: 1641-1674.
  4. Ikonen, T., Bovenhuis, H., Ojala, M., Ruottinen, O. and Georges, M. (2001). Associations between casein haplotypes and first lactation milk production traits in Finnish Ayrshire cows. Journal of Dairy Science. 84: 507-514.
  5. John, S.W., Weitzner, G., Rozen, R. and Scriver, C.R. (1991). A rapid procedure for extracting genomic DNA from leukocytes. Nucleic Acid Research. 19 (2): 408.
  6. Mir, N.S., Ullah, O. and Sheikh, R. (2014). Genetic polymorphism of milk protein variants and their association studies with milk yield in Sahiwal cattle. African Journal of Biotechnology. 13 (4): 555-565.
  7. Sodhi, M., Manishi, M., Ranjit, S.K., Mishra., B.P. and Balwinder, K. (2012). Milk proteins and human health: A1/A2 milk hypothesis. Indian Journal of Endocrinology and Metabolism. 16(5): 856.
  8. Shende, T.C., Kulkarni, P.S. and Pawar, P.C. (2017). Genotyping of HF crossbred cattle for β-casein genes using PCR-RFLP. Indian Research Journal of Extension Education (special issue on Veterinary Research and Extension). 17: 105-107.
  9. Snedecor, G.W. and Cochran, W.G. (1994). Statistical method. 8th edn. The lowa State College Press, Inc. Amer. lowa USA. 950p.
  10. Szymanowsky, M., Eulalia, S., Marek, L. and Lech, Z. (2004). Association of nucleotide-sequence polymorphism in the 5’-flanking regions of bovine casein genes with casein content in cow’s milk. INRAEDP Sciences. 84: 579-590.
  11. Truswell, A.S. (2005). The A2 milk case. European Journal of Clinical Nutrition. 59: 623-631.
  12. Yeh, F.C., Yang, R.C., Boyle, T.B.J., Ye, Z.H. and Mao, J.X. (1999). Popgene 32 version 1.32, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.

Global Footprints