Loading...

Effect of Dietary Copper and Zinc Supplementation on Semen Quality of Murrah Bulls

DOI: 10.18805/ijar.B-3887    | Article Id: B-3887 | Page : 1260-1264
Citation :- Effect of Dietary Copper and Zinc Supplementation on Semen Quality of Murrah Bulls.Indian Journal of Animal Research.2020.(54):1260-1264
Abhradip Majumder, Manjula Thakur, Mukesh Bhakat, Manorama Saha, Tushar Kumar Mohanty, Goutam Mondal gmondal1075@gmail.com
Address : Animal Nutrition Division, ICAR-National Dairy Research Institute, Karnal-132 001, Haryana, India.
Submitted Date : 24-07-2019
Accepted Date : 1-01-2020

Abstract

The effect of dietary supplementation of Cu and Zn on semen quality parameters and certain bio-chemicals parameters were evaluated in Murrah bulls. Twelve mature Murrah bulls (4-6 years of age) were divided into three groups (n=4) T1, T2, and T3 based on semen volume and concentration and were fed as per ICAR standard (2013). However, the animals were supplemented with 0%, 25%, and 50% Cu and Zn above the basal diet in T1, T2 and T3 groups, respectively for 180 days. Quantitative and qualitative characteristics of semen, blood and seminal plasma antioxidant status, blood and semen minerals (Cu, Zn, Ca and Mn) were determined in experimental Murrah bulls. Semen ejaculate volume (mL) increased in T2 and T3 while sperm concentration (million/mL), intact acrosome (%), HOST reacted spermatozoa (%) increased in T3 group compared to T1 and T2 groups. No difference was observed in mass motility, pH, live spermatozoa (%) in semen sexual behaviour except dismounting time which was decreased in T3 than T1 and T2 (P<0.05). SOD activity in blood plasma and LPO activity decreased in seminal plasma in both T2 and T3 groups than T1, whereas catalase activity did not show any significant difference. Cu and Zn supplementation in T2 group improved (P<0.05) Zn level in blood and seminal plasma and Cu level in blood plasma only, but not in seminal plasma. Therefore, it can be concluded that supplementation of Zn and Cu at 50% above the recommended levels of ICAR (2013) improved the qualitative and quantitative attributes of semen in Murrah bulls.

Keywords

Copper Murrah bulls Zinc Semen quality.

References

  1. Aebi, H. (1984). Catalase in vitro. In: Methods in Enzymology (L. Packer; ed.). Academic Press, New York. Pp 121-126.
  2. Ahmed, S., Cajat, A.K., Albanell, E., Snch, X. and Casals, R. (2003). Effects of dietary supplements of zinc-methionine on milk production, udder health and zinc metabolism in dairy goats. Journal of Dairy Research. 70:9-17.
  3. Anzar, M., Ahmad, M., Nazir, M., Ahmad, N., Shah, I.H. (1993). Selection of buffalo bulls: Sexual behaviour and its rela    tionship to semen production and fertility. Theriogenology. 40:1187-1198.
  4. AOAC. (2005). Official Methods of Analysis 18th edn. Association of Official Analytical Chemists. Maryland, USA.
  5. Eidi, M., Eidi, A., Pouyan, O., Shahmohammadi, P., Fazaeli, R., Bahar, M. (2010). Seminal plasma levels of copper and its relationship with seminal parameters. Iranian Journal of Reproductive Med-icine. 8 (2): 60-65.
  6. Garg, A.K., Mudgal, V., Dass, R.S. (2008). Effect of organic zinc supplementation on growth, nutrient utilization and min    eral profile in lambs. Animal feed science and technol    ogy. 144(1-2):82-96.
  7. ICAR. (2013). Nutrient requirements of cattle and buffalo, Indian Council of Agricultural Research, New Delhi, India.
  8. Jeyendran, R.S., Vanderven H., Perezpelaez, M., Crabo, B.G., Zaneveld, L.J.D. (1984). Development of an assay to assess the functional integrity of the human sperm mem    brane and its relationship to other semen characteris    tics. Journal of Reproduction and Fertility. 70:219-228.
  9. Kumar, N., Vermaa, R.P., Singh, L.P., Varshney, V.P., Dass, R.S. (2006). Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum tes-tosterone level in crossbred cattle (Bosindicus x Bostaurus) bulls. Reproduction Nu    trition and De-velopment. 46 (6): 663-75.
  10. Madesh, M., and Balasubramanian, K.A. (1998). Microtiter plate assay for superoxide dismutase using reduction by superoxide. Indian Journal of Biochemistry and Biophysics. 35 (3):184-188.
  11. Martin, G.B., White, C.L., Markey, C.M., Blackberry, M.A. (1994). Effect of dietary zinc deficiency on the reproductive sys    tem of young male sheep: testicular growth and the se cretion of inhibin and testosterone. Journal of Reproduc tion and Fertility. 101: 87-96.
  12. NDDB. (2012). National Dairy Plan Phase I. National Dairy Devel opment Board. Manual on Semen Production.
  13. Nocek, J.E., Socha, M.T., Tomlinson, D.J. (2006). The effect of trace mineral fortification level and source on perfor    mance of dairy cattle. Journal of Dairy Science. 89(7): 2679-2693.
  14. NRC. (2001). Nutrient Requirement of Dairy Cattle, 7th revised edn. National Research Council Na-tional Academy Press, Washington, DC.
  15. Rowe, M., Powell, J., Kegley, E., Lester, T., Rorie, R. (2014). Effect of supplemental trace mineral source on bull semen quality. The Professional Animal Scientist. 30:68-73.
  16. Roy, B. (2006). Influence of zinc supplementation on semen quality and sexual behaviour of Cross-bred and Murrah buffalo bulls. Ph.D Thesis, National Dairy Research Institute, Karnal, Haryana.
  17. Roy, B., Bhagel, R.P.S., Mondal, G. (2013). Effect of zinc on male reproduction: A review. Indian Journal of Animal Nutrition. 30:339-350.
  18. Sabhapati, M, Raina,V.S., Bhakat, M., Mohanty, T.K., Shivahra, P.R., Mondal, G., Gupta, A.K. (2015). Improvement of sexual behaviour and semen quality by therapeutic approach and zinc supplementation on Karan Fries. Indian Journal of Animal Sciences. 86 (6): 655-658.
  19. Sharma, M.C. Raju, S., Joshi, C., Kaur, H., Varshney, V.P. (2003). Studies on serum micro-mineral, homone and vitamin profile and its effect on production and therapeutic man    agement of buffaloes in Haryana state of India. Asian-Australasian Journal of Animal Sciences. 16 (4): 519-528.
  20. Si, Y.M., Zaug, Z.C., An, M., Lu, X.L., Lu, Q., Wang, L. (1990). Effect of zinc supplementation of the feed on quality of frozen semen from Holstein bulls. Chinese Journal of Animal Sciences. 26: 9-12.
  21. Suleiman, S.A., Ali, M.E., Zaki, Z.M., Malik, E.M., Nasar, M.A. (1996). Lipid peroxidation and human sperm motility: protective role of vitamin E. Asian Journal of Andrology. 17: 530-537.
  22. Wong, W.Y., Merkus, H.M., Thomas, C.M., Menkveld, R., Zielthuis, G.A., Steegers, Theunissen, R.P. (2002). Effect of folic acid and zinc sulphate on male factor sub fertility, a double blind, randomized placed controlled trial. Fertility and Sterility. 77(3): 491-98.

Global Footprints