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Effect of Kisspeptin on in vitro Maturation of Buffalo Oocytes

DOI: 10.18805/ijar.B-3884    | Article Id: B-3884 | Page : 1218-1222
Citation :- Effect of Kisspeptin on in vitro Maturation of Buffalo Oocytes.Indian Journal of Animal Research.2020.(54):1218-1222
Kolipaka Rajesh, B. Swathi, G. Aruna Kumari, M. Shanmugam rajesh0929@gmail.com
Address : Department of Veterinary Physiology, College of Veterinary Science, Hyderabad-500 030, India. 
Submitted Date : 23-07-2019
Accepted Date : 21-11-2019

Abstract

To explore the effect of kisspeptin on in vitro maturation of buffalo oocytes under different hormonal combinations. For the present study, Kp@10 µg/ml was selected as optimum by assessing the proportion of Cumulus Cell Expansion (CCE) and extrusion of 1st polar body (PB) from the earlier study conducted. Media containing TCM 199 (supplemented with Gentamicin) and oocytes were used as control. Overall, oocytes were allowed to mature in 12 different maturation media viz., Control (T1), Kp at 10 µg/ml (T2), FSH alone (T3), FSH +Kp  (T4), LH (T5), LH +Kp (T6), E2 (T7), E2+Kp (T8), FSH+LH+E2 (T9), FSH+LH+E2+Kp (T10), FSH+LH+E2+FCS+BSA (T11) and FSH+LH+E2+FCS+BSA+Kp (T12), incubated at 38.5°C and 5% CO2 under humidified atmosphere for 22 hours. The results showed significantly (P £ 0.5) higher percentages of CCE and PB in groups supplemented with Kp (T4, T6, T8, T10 and T12,) compared to control (T1) and other treatment groups supplemented with individual hormones either alone (T3, T5 and T7) or combination of hormones (T9) and IVM alone (T11). Therefore, it has been clearly demonstrated that addition of Kp (10 µg/ml) to FSH, LH, E2 supplemented media, in various combinations enhances buffalo oocyte maturation rates in vitro. 

Keywords

Buffalo oocytes In vitro maturation Kisspeptin Polar body extrusion

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