Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 10 (october 2020) : 1265-1271

Characterization of Escherichia coli Isolated from Bovine Subclinical Mastitis for Virulence Genes, Phylogenetic Groups and ESBL Production

S.M. Nalband, R.P. Kolhe, P.D. Deshpande, S.N. Jadhav, D.G. Gandhale, D.M. Muglikar, S.R. Kolhe, S.S. Bhave, U.V. Jagtap, C.V. Dhandore
1Department of Veterinary Public Health, KNP College of Veterinary Science Shirwal, Satara-412 801, Maharashtra, India.
Cite article:- Nalband S.M., Kolhe R.P., Deshpande P.D., Jadhav S.N., Gandhale D.G., Muglikar D.M., Kolhe S.R., Bhave S.S., Jagtap U.V., Dhandore C.V. (2020). Characterization of Escherichia coli Isolated from Bovine Subclinical Mastitis for Virulence Genes, Phylogenetic Groups and ESBL Production. Indian Journal of Animal Research. 54(10): 1265-1271. doi: 10.18805/ijar.B-3883.

ABSTRACT

Pathogenic E. coli associated with bovine mastitis are least studied group of microbes in India. Thus an investigation was carried out to know the occurrence of bovine subclinical mastitis and E. coli at different dairy farms. E. coli strains isolated from clinical and environmental samples were screened for virulent genes namely, eae, bfpA, iapH, aggR, elt, est, stx1/stx2; phylogenetic groups and for ESBL production. Findings revealed high prevalence of subclinical mastitis (45%). E. coli strains were isolated from milk, udder, milking machine, floor swabs, etc. Out of 81 E. coli, 38 (46.91%) were ESBL producers. The virulent genes in single or in combination were detected in 55 strains and eae gene was predominant (43.20%). The stx1/stx2 were detected in 2.47% samples. E. coli isolates fall under phylogenetic groups B1 (58.18%), A (23.63%) and D (18.18%). Phylogenetic group B2 was not detected. Dairy cattle could act as a reservoir of ESBL type diarrheagenic E. coli. 

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