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Research Article

volume 52 issue 2 (february 2018) : 226-231,   Doi: 10.18805/ijar.B-387

Studying the antioxidant role of mushroom against hazards of gamma radiation exposure in male rats

R
R.G. Hamza
A
A.N.El Shahat
M
M.N. Al-seeni
1Food Irradiation Research Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
Cite article:- Hamza R.G., Shahat A.N.El, Al-seeni M.N. (2018). Studying the antioxidant role of mushroom against hazards of gamma radiation exposure in male rats. Indian Journal of Animal Research. 52(2): 226-231. doi: 10.18805/ijar.B-387.

ABSTRACT

This study aimed to investigate the antioxidant role of dried mushroom (DM) against hazards of gamma-irradiation in male rats. In this study, exposure of rats to whole body g-radiation (6 Gy) resulted in hepatic oxidative stress (a significant increase in lipid peroxidation concomitant with a significant decrease in glutathione content and antioxidant enzyme activities); increase liver function enzymes and histopathological disorders. Treatment of irradiated rats with 10% DM significantly improved radiation-induced injury as indicated by the reduction of the indices of liver damage, lipid peroxidation product, the elevation of antioxidants and the attenuation of the tissues histological architecture. These results suggest that oyster mushroom can improve the antioxidant status and minimize the occurrence of oxidative stress- associated disorders.

REFERENCES

  1. A.O.A.C. (2000). Official Methods of Analysis of Association of Official Analytical Chemists. 17th ed. Washington D.C., USA 2000.
  2. Abdelazim, A.M. and Afifi. M. (2013). Oyster mushroom (Pleurotus ostreatus) strain 238 ameliorates the oxidative stress in STZ-    induced diabetic mice. Life Sci. J. , 10(3): 1259-1264
  3. Abdelhalim, M.A.K. and Moussa. S.A.A.(2013). The biochemical changes in rats’ blood serum levels exposed to different g- radiation doses. Afr. J. Pharm. Pharmacol., 7(15):785-792.
  4. Acharya, K., Giri. S. and Biswas. G.(2011). Comparative study of antioxidant activity and nitric oxide synthase activation property of different extracts from Rhododendron arboreum flower. Int.J. PharmTech Res., 3(2): 757-762.
  5. Alam, N., Yoon, K.N., Cha, Y.J., Kim, J.H., Lee, K.R. , Lee,T.S. (2011). Appraisal of the antioxidant, phenolic compounds concentration, xanthine oxidase and tyrosinase inhibitory activities of Pleurotus salmoneostramineus. Afr. J. Agric. Res., 6(6):1555-1563.
  6. Ali, S.A., Fadda, L.M., Elebiary. H. and Soliman.M. (2012). Evaluation of the radioprotective action of anserine along with zinc in albino rats exposed to g- radiation. J. of Applied Pharmace. Sci., 02 (04): 115-122.
  7. Azab, K. Sh., Mostafa, A.H., Ali, E.M. and Abdel-Aziz, M.A.(2011). Cinnamon extract ameliorates ionizing radiation-induced cellular injury in rats. Ecotoxicol Environ. Saf., 74: 2324- 2329.
  8. Beutler, E., Duron,O. and Kelly, B.M. (1963). Improved method for the determination of blood glutathione. J. Lab. Clin. Med., 61: 882-888.
  9. Choudhury, M.B.K., Hassan, M.M., Mowsumi, F.R., Sarker, N.C., Hossain, M.S. and Choudhury, M.S.K.(2011). Oyster mushroom ameliorates liver function during ramadan fast reflected by reducing the serum level of alanine aminotransferase and aspartate aminotransferase of male volunteers. Int. J. Nutr. Metab., 3(10): 123-127.
  10. Costantino, L., Albasini, A., Rastelli, G. and Benvenuti, S. (1992). Activity of polyphenolic crude extracts as scavengers of superoxide radicals and inhibitors of xanthine oxidase. Planta Med., 58: 342-344.
  11. Devi, L.S., Dasgupta, A., Chakraborty, M., Borthakur, S.K. and Singh, N.I. (2014). Chemical Composition and Antioxidant Activity of Schizophyllum Commune. Int. J. Pharm. Sci. Rev. Res., 27(2): 173-177.
  12. El shahat, A.N. (2013). Efficiency of Broccoli in Attenuating of Some Biochemical Disorders in Rats exposed to g-irradiation. Arab J. Nuc. Sci. Applic., 46(4): 260-267.
  13. Gaafar, A.M., Yossef, H.E. and Ibrahim, H.H.(2010). Protective effects of mushroom and their ethyl extract on aging compared with L-    carnitine. Int. J. Nutr. Metab., 2(4):63-69.
  14. Hu, M.L. (2011). Dietary polyphenols as antioxidants and anticancer agents: more questions than answers. Chang Gung Med. J.; 34: 449–460. 
  15. Jayakumar, T., Ramesh, E. and Geraldine,P.(2006). Antioxidant activity of the oyster mushroom, Pleurotus ostreatus, on CCl4-induced liver injury in rats. Food Chem. Toxicol., 44: 1989–1996.
  16. Johansson, L.H. and Borg, L.A.H. (1988). A spectrophotometric method for determination of catalase activity in small tissue samples. Anal. Biochem., 174: 331.
  17. Kaminski, Z.W. and Jewezska, M.M. (1979). Intermediate dehydrogenase oxidase form of xanthine oxidoreductase in rat liver. Biochem J ., 181: 177–182.
  18. Kind, P. and King, E. (1954). Estimation of plasma phosphatase by determination of hydrolysed phenol with aminoantipyrine. J. Clin. Pathol., 7: 322-326. 
  19. Kregel, K.C. and Zhang, H.J. (2007). An integrated view of oxidative stress in aging: basic mechanisms, functional effects,and pathological considerations. Am. J. Physiol. Regul. Integr. Comp. Physiol., 292: 18–36.
  20. Lakshmi, B., Tilak, J.C., Adhikari, S., Devasagayam, T.P.A. and Janardhanan, K.K. (2004). Evaluation of antioxidant activity of selected Indian mushrooms. Pharm. Biol., 42:179–185.
  21. Lane–Peter, W. and Pearson, A.E. (1971). Dietary require. In: “The laboratory animal principles and practice”. Academic Press London and New York. 
  22. Makhlouf, R. and Makhlouf, I. (2012). Evaluation of the effect of Spirulina against Gamma irradiation induced oxidative stress and tissue injury in rats. Int. J. Appl. Sci. Eng. Res., 1(2):152-164.
  23. Mansour, H.H. and Hafez, H.F. (2012). Protective effect of Withania somnifera against radiation induced hepato-toxicity in rats .Ecotoxicol. Environ. Saf., 80: 14–19.
  24. Minami, M. and Yoshikawa. H. (1979). A simplified assay method of superoxide dismutase. Clin. Chem. Acta., 92: 337.
  25. Mishra, S. and Singh. R.B. (2010). Effect of Mushroom on the Lipid Profile, Lipid Peroxidation and Liver Functions of Aging Swiss Albino Rats. The Open Nutraceuticals J., 3:248-253.
  26. Orhan, D.D., Orhan, N., Ergun, E. and Ergun, F. (2007). Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride induced acute liver damage in rats. J Ethnopharmacol., 112(1):145.
  27. Pal, J., Ganguly, S., Tahsin, K.S. and Acharya, K. (2010). In vitro free radical scavenging activity of wild edible mushroom,Pleurotus squarrosulus (Mont.) Singer. Indian J. Exp. Biol., 48:1210-121.
  28. Philip, G.R., Forrest, H.N. and George, C.F. (1993). AIN – 76A rodent diet. J. of Nutr., 123:1935-1951.
  29. Pulpanova, J., Kovarova, H. and M. (1982). Ledvina Changes in the reduced glutathione concentration and the activity of glutathione reductase in some tissues of the rats gamma- irradiated after the administration of cystamine. Radiobiol. Radiother. 23(1):50-6.
  30. Punitha, S.C. and Karan, M.R. (2014). Free radical scavenging activity of fruiting body extracts of an edible mushroom, Volvariella volvacea (Bull. ex Fr.) Singer: an in vitro study. Asian J. Phar. Biol. Res., 4(30): 6-11.
  31. Reitman, S. and Frankel, S. (1957). A calorimetric method for determination of serum transaminases. Am. J. Clin. Pathol., 28: 56-63.
  32. Schneeman, B.O., Rico, R. and Richter, B.D.(1989). Reduction of plasma and hepatic triglyceride with whole milk containing diet in rats. J. of Nutr., 119: 956-970.
  33. Singh, N.I., Devi, L.S. , Ghosh, S., Das, A. and Borthakur, S.K. (2014). Antioxidative and free radical scavenging activity of ethanolic extract of Lentinula edodes. Asian J. Pharm. Clin. Res., 7(3):195-199.
  34. SPSS. (1998). Statistical Package for Social Science. Computer Software, Ver. 10. SPSS Company, London, UK.
  35. Srivastava, M.,Chandra, D. and Kale, R.K. (2002). Modulation of radiation-induced changes in the xanthine oxidoreductase system in the livers of mice by its inhibitors. Radiat Res., 157(3):290-7.
  36. Synytsya, A., Mickova, K., Synytsya, A ., Jablonsky, I. , Spevacek, J., Erban, V., Kovarikova, E. and J. Copikova. (2009). Glucans from fruit bodies of cultivated mushrooms Pleurotus ostreatus and Pleurotus eryngii: structure and potential prebiotic activity. Carbohyd. Polym., 76: 548-556.
  37. El-kholy, T.A., Naglaa, H.M. Hassanen and Abbas, H.Y. (2013). Protection of the Mushroom (shiitake”Lentinus-edodes) against Carbon-Tetrachloride-Induced Renal Injury in Rats. Life Sci J, 10(1):1701-1708.
  38. Williams, R., Spencer, J. and Rice Evans, C. (2004). Flavonoids: antioxidants or signalling molecules? free radic. Biol. Med. 36: 838–849.
  39. Yoshioka, T., Kawada, K., Shimada, T. and Mori, M. (1979). Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood, Am. J. Obstet. Gynecol. 135: 372.
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Indian Journal of Animal Research
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    Research Article

    volume 52 issue 2 (february 2018) : 226-231,   Doi: 10.18805/ijar.B-387

    Studying the antioxidant role of mushroom against hazards of gamma radiation exposure in male rats

    R
    R.G. Hamza
    A
    A.N.El Shahat
    M
    M.N. Al-seeni
    1Food Irradiation Research Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
    Cite article:- Hamza R.G., Shahat A.N.El, Al-seeni M.N. (2018). Studying the antioxidant role of mushroom against hazards of gamma radiation exposure in male rats. Indian Journal of Animal Research. 52(2): 226-231. doi: 10.18805/ijar.B-387.

    ABSTRACT

    This study aimed to investigate the antioxidant role of dried mushroom (DM) against hazards of gamma-irradiation in male rats. In this study, exposure of rats to whole body g-radiation (6 Gy) resulted in hepatic oxidative stress (a significant increase in lipid peroxidation concomitant with a significant decrease in glutathione content and antioxidant enzyme activities); increase liver function enzymes and histopathological disorders. Treatment of irradiated rats with 10% DM significantly improved radiation-induced injury as indicated by the reduction of the indices of liver damage, lipid peroxidation product, the elevation of antioxidants and the attenuation of the tissues histological architecture. These results suggest that oyster mushroom can improve the antioxidant status and minimize the occurrence of oxidative stress- associated disorders.

    REFERENCES

    1. A.O.A.C. (2000). Official Methods of Analysis of Association of Official Analytical Chemists. 17th ed. Washington D.C., USA 2000.
    2. Abdelazim, A.M. and Afifi. M. (2013). Oyster mushroom (Pleurotus ostreatus) strain 238 ameliorates the oxidative stress in STZ-    induced diabetic mice. Life Sci. J. , 10(3): 1259-1264
    3. Abdelhalim, M.A.K. and Moussa. S.A.A.(2013). The biochemical changes in rats’ blood serum levels exposed to different g- radiation doses. Afr. J. Pharm. Pharmacol., 7(15):785-792.
    4. Acharya, K., Giri. S. and Biswas. G.(2011). Comparative study of antioxidant activity and nitric oxide synthase activation property of different extracts from Rhododendron arboreum flower. Int.J. PharmTech Res., 3(2): 757-762.
    5. Alam, N., Yoon, K.N., Cha, Y.J., Kim, J.H., Lee, K.R. , Lee,T.S. (2011). Appraisal of the antioxidant, phenolic compounds concentration, xanthine oxidase and tyrosinase inhibitory activities of Pleurotus salmoneostramineus. Afr. J. Agric. Res., 6(6):1555-1563.
    6. Ali, S.A., Fadda, L.M., Elebiary. H. and Soliman.M. (2012). Evaluation of the radioprotective action of anserine along with zinc in albino rats exposed to g- radiation. J. of Applied Pharmace. Sci., 02 (04): 115-122.
    7. Azab, K. Sh., Mostafa, A.H., Ali, E.M. and Abdel-Aziz, M.A.(2011). Cinnamon extract ameliorates ionizing radiation-induced cellular injury in rats. Ecotoxicol Environ. Saf., 74: 2324- 2329.
    8. Beutler, E., Duron,O. and Kelly, B.M. (1963). Improved method for the determination of blood glutathione. J. Lab. Clin. Med., 61: 882-888.
    9. Choudhury, M.B.K., Hassan, M.M., Mowsumi, F.R., Sarker, N.C., Hossain, M.S. and Choudhury, M.S.K.(2011). Oyster mushroom ameliorates liver function during ramadan fast reflected by reducing the serum level of alanine aminotransferase and aspartate aminotransferase of male volunteers. Int. J. Nutr. Metab., 3(10): 123-127.
    10. Costantino, L., Albasini, A., Rastelli, G. and Benvenuti, S. (1992). Activity of polyphenolic crude extracts as scavengers of superoxide radicals and inhibitors of xanthine oxidase. Planta Med., 58: 342-344.
    11. Devi, L.S., Dasgupta, A., Chakraborty, M., Borthakur, S.K. and Singh, N.I. (2014). Chemical Composition and Antioxidant Activity of Schizophyllum Commune. Int. J. Pharm. Sci. Rev. Res., 27(2): 173-177.
    12. El shahat, A.N. (2013). Efficiency of Broccoli in Attenuating of Some Biochemical Disorders in Rats exposed to g-irradiation. Arab J. Nuc. Sci. Applic., 46(4): 260-267.
    13. Gaafar, A.M., Yossef, H.E. and Ibrahim, H.H.(2010). Protective effects of mushroom and their ethyl extract on aging compared with L-    carnitine. Int. J. Nutr. Metab., 2(4):63-69.
    14. Hu, M.L. (2011). Dietary polyphenols as antioxidants and anticancer agents: more questions than answers. Chang Gung Med. J.; 34: 449–460. 
    15. Jayakumar, T., Ramesh, E. and Geraldine,P.(2006). Antioxidant activity of the oyster mushroom, Pleurotus ostreatus, on CCl4-induced liver injury in rats. Food Chem. Toxicol., 44: 1989–1996.
    16. Johansson, L.H. and Borg, L.A.H. (1988). A spectrophotometric method for determination of catalase activity in small tissue samples. Anal. Biochem., 174: 331.
    17. Kaminski, Z.W. and Jewezska, M.M. (1979). Intermediate dehydrogenase oxidase form of xanthine oxidoreductase in rat liver. Biochem J ., 181: 177–182.
    18. Kind, P. and King, E. (1954). Estimation of plasma phosphatase by determination of hydrolysed phenol with aminoantipyrine. J. Clin. Pathol., 7: 322-326. 
    19. Kregel, K.C. and Zhang, H.J. (2007). An integrated view of oxidative stress in aging: basic mechanisms, functional effects,and pathological considerations. Am. J. Physiol. Regul. Integr. Comp. Physiol., 292: 18–36.
    20. Lakshmi, B., Tilak, J.C., Adhikari, S., Devasagayam, T.P.A. and Janardhanan, K.K. (2004). Evaluation of antioxidant activity of selected Indian mushrooms. Pharm. Biol., 42:179–185.
    21. Lane–Peter, W. and Pearson, A.E. (1971). Dietary require. In: “The laboratory animal principles and practice”. Academic Press London and New York. 
    22. Makhlouf, R. and Makhlouf, I. (2012). Evaluation of the effect of Spirulina against Gamma irradiation induced oxidative stress and tissue injury in rats. Int. J. Appl. Sci. Eng. Res., 1(2):152-164.
    23. Mansour, H.H. and Hafez, H.F. (2012). Protective effect of Withania somnifera against radiation induced hepato-toxicity in rats .Ecotoxicol. Environ. Saf., 80: 14–19.
    24. Minami, M. and Yoshikawa. H. (1979). A simplified assay method of superoxide dismutase. Clin. Chem. Acta., 92: 337.
    25. Mishra, S. and Singh. R.B. (2010). Effect of Mushroom on the Lipid Profile, Lipid Peroxidation and Liver Functions of Aging Swiss Albino Rats. The Open Nutraceuticals J., 3:248-253.
    26. Orhan, D.D., Orhan, N., Ergun, E. and Ergun, F. (2007). Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride induced acute liver damage in rats. J Ethnopharmacol., 112(1):145.
    27. Pal, J., Ganguly, S., Tahsin, K.S. and Acharya, K. (2010). In vitro free radical scavenging activity of wild edible mushroom,Pleurotus squarrosulus (Mont.) Singer. Indian J. Exp. Biol., 48:1210-121.
    28. Philip, G.R., Forrest, H.N. and George, C.F. (1993). AIN – 76A rodent diet. J. of Nutr., 123:1935-1951.
    29. Pulpanova, J., Kovarova, H. and M. (1982). Ledvina Changes in the reduced glutathione concentration and the activity of glutathione reductase in some tissues of the rats gamma- irradiated after the administration of cystamine. Radiobiol. Radiother. 23(1):50-6.
    30. Punitha, S.C. and Karan, M.R. (2014). Free radical scavenging activity of fruiting body extracts of an edible mushroom, Volvariella volvacea (Bull. ex Fr.) Singer: an in vitro study. Asian J. Phar. Biol. Res., 4(30): 6-11.
    31. Reitman, S. and Frankel, S. (1957). A calorimetric method for determination of serum transaminases. Am. J. Clin. Pathol., 28: 56-63.
    32. Schneeman, B.O., Rico, R. and Richter, B.D.(1989). Reduction of plasma and hepatic triglyceride with whole milk containing diet in rats. J. of Nutr., 119: 956-970.
    33. Singh, N.I., Devi, L.S. , Ghosh, S., Das, A. and Borthakur, S.K. (2014). Antioxidative and free radical scavenging activity of ethanolic extract of Lentinula edodes. Asian J. Pharm. Clin. Res., 7(3):195-199.
    34. SPSS. (1998). Statistical Package for Social Science. Computer Software, Ver. 10. SPSS Company, London, UK.
    35. Srivastava, M.,Chandra, D. and Kale, R.K. (2002). Modulation of radiation-induced changes in the xanthine oxidoreductase system in the livers of mice by its inhibitors. Radiat Res., 157(3):290-7.
    36. Synytsya, A., Mickova, K., Synytsya, A ., Jablonsky, I. , Spevacek, J., Erban, V., Kovarikova, E. and J. Copikova. (2009). Glucans from fruit bodies of cultivated mushrooms Pleurotus ostreatus and Pleurotus eryngii: structure and potential prebiotic activity. Carbohyd. Polym., 76: 548-556.
    37. El-kholy, T.A., Naglaa, H.M. Hassanen and Abbas, H.Y. (2013). Protection of the Mushroom (shiitake”Lentinus-edodes) against Carbon-Tetrachloride-Induced Renal Injury in Rats. Life Sci J, 10(1):1701-1708.
    38. Williams, R., Spencer, J. and Rice Evans, C. (2004). Flavonoids: antioxidants or signalling molecules? free radic. Biol. Med. 36: 838–849.
    39. Yoshioka, T., Kawada, K., Shimada, T. and Mori, M. (1979). Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood, Am. J. Obstet. Gynecol. 135: 372.
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