Banner
Current IssueEditorial BoardOnline First Articles
Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Research Article

Indian Journal of Animal Research, volume 54 issue 8 (august 2020) : 1012-1017

Molecular characterization and histopathological studies on Fasciola gigantica in Mithun (Bos frontalis)

J.K. Chamuah, D. Borkotoky, Amenti, K. Khate, S.S Jacob, Lalchamliani, O.K. Raina, M.H Khan, A. Mitra
1ICAR-National Research Centre on Mithun, Medziphema-797 106, Nagaland, India.
Cite article:- Chamuah J.K., Borkotoky D., Amenti, Khate K., Jacob S.S, Lalchamliani, Raina O.K., Khan M.H, Mitra A. (2019). Molecular characterization and histopathological studies on Fasciola gigantica in Mithun (Bos frontalis). Indian Journal of Animal Research. 54(8): 1012-1017. doi: 10.18805/ijar.B-3856.

ABSTRACT

Mitochondrial DNA sequence of the sub-unit 1 of cytochrome c oxidase (Cox1) and ribosomal DNA sequence of the first internal transcribed spacer (ITS-1) of Fasciola collected from mithun and cattle from Arunachal Pradesh, India were characterized at molecular level in order to identify the species of parasite. Based on sequence and phylogenetic analysis, the identity of the parasite was confirmed as Fasciola gigantica in mithun as well as from cattle. In order to know histopathological alteration in Fasciola infection, a histopathological study was performed on eight liver specimens, out of 110 animals studied during the period from 2010-2016. Histopathological examination of F.gigantica infected liver showed extensive fibrous connective tissue proliferation with necrosis of hepatocytes and infiltration of  polymorphonuclear cell. There was evidence of migratory  tracts of parasites with losing of normal lobular hepatic architecture. Bile duct proliferation was followed by congestion of portal vein with perivascular cuffing with surrounding degeneration of hepatocytes. The hepatocytes showed pyknosis with hyperplasia of bile duct. 

REFERENCES

  1. Ashrafi, K., Valero, M.A., Panova, M., Periago, M.V., Massoud, J. and, Mas-Coma, S., (2006). Phenotypic analysis of adults of Fasciola hepatica, Fasciola gigantica and intermediate forms from the endemic region of Gilan, Iran. Parasitol. Int. 55(4): 249–260.
  2. Bal, M.S., Sharma, A., Ashuma, Bath, B.K., Kaur, P. and Singla, L.D. (2014) Detection and management of latent infection of Trypanosoma evansi in a cattle herd. Indian J. Anim. Res. 48(1): 31-37.
  3. Chamuah, J. K., Das M., Islam, S., Rajkhowa, S., Rajkhowa, C., Chakraborty, A. (2009). Studies on naturally acquired gastrointestinal helminth of mithun (Bos frontalis). J.Vet. Parasitol. 23:37-40.
  4. Chamuah, J. K., Jacob, S.S., Sakhrie, A., Borkotoky, D. (2014). Serological Prevalence of Fasciola gigantica in Mithun (Bos frontalis). Int. J. Livest. Res. 4(5):15-20. 
  5. Chamuah, J. K., Perumal, P., Singh, V., Mech, A., Borkotoky, D. (2013a). Helminth parasites of mithun (Bos frontalis) -An overview. Indian J. Anim. Sci. 83(3):235–237.
  6. Chamuah, J. K., Sakhrie, A., Lama, S., Chandra, S., Chigure, G. M., Bauri, R. K., Jacob, S. S. (2015). Molecular characterization of Setaria digitata from Mithun (Bos frontalis). Acta Parasitol. 60(3): 391–394.
  7. Chamuah, J. K., Pegu, S.R., Raina, O.K., Siju, S.J., Sakhrie, A., Deka, A., Rajkhowa C.(2016b). Pimply gut condition in mithun (Bos frontalis) calves. J Parasit. Dis. 40(2):252-254.
  8. Chamuah, J.K. (2005). Studies on some aspects of parasites of mithun (Bos frontalis). M.V.Sc. Thesis submitted to Assam Agricultural University, Khanapara, and Guwahati-22
  9. Chamuah, J.K., Dutta, B., Borkotoky, D. (2017). Pathological studies on helminth parasitic infection in mithun (Bos frontalis). J. Parasit. Dis. 41(4): 929-932.
  10. Chamuah, J.K., Jacob, S. S., Lalkrima, H., Sankar, M., Banerjee, P.S., Raina O. K. (2016a). Molecular characterization of veterinary important trematode and cestode species in the mithun (Bos frontalis) from north-east India. J. Helminthol. 90(5): 577-582. 
  11. Chamuah, J.K., Singh, V., Dutta, P.R., Khate, K., Mech, A., Rajkhowa, C. and Borkotoky, D. (2013b). Incidence of gastrointestinal helminths parasites in free ranging mithun (Bos frontalis) from Phek district of Nagaland. Indian Vet J. 90(3): 127-128. 
  12. Chhabra, M.B. and Singla, L.D. (2009). Food-borne parasitic zoonoses in India: Review of recent reports of human infections. J. Vet. Parasitol. 23(2): 103-110.
  13. Gajewska, A., Smaga-Koz³owska K, Wiœniewski, M. (2005). Pathological changes of liver in infection of Fasciola hepatica. Wiad Parazytol. 51(2):115-23.
  14. Haroun, E.M. and Hussein, M.F. (1975). Clinico-pathological studies on naturally-occurring bovine fascioliasis in the Sudan. J. Helminthol. 49(3): 143-152.
  15. Huang, W.Y., He, B., Wang, C.R., Zhu, X.Q. (2004). Characterisation of Fasciola species from Mainland China by ITS-2 ribosomal DNA sequence. Vet. Parasitol. 120(1-2):75–83.
  16. Itagaki, T., Kikawa, M., Sakaguchi, K., Shimo, J., Terasaki, K., Shibahara, T., Fukuda, K. (2005). Genetic characterization of parthenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA. Parasitol. 131(Pt5): 679–685.
  17. Itagaki, T., Sakaguchi, K., Terasaki, K., Sasaki O., Yoshihara, S., Van Dung, T. (2009). Occurrence of spermic diploid and aspermic triploid forms of Fasciola in Vietnam and their molecular characterization based on nuclear and mitochondrial DNA. Parasitol. Int. 58(1): 81–85.
  18. Kaur, P., Juyal, P.D., Sharma, A., Bal, M.S. and Singla, L.D. (2016). Seroprevalence of Babesia bigemina in dairy animals from low lying regions of Punjab. Indian J. Anim Res. 50 (3):406-410. 
  19. Kaur, S., Singla, L.D., Sandhu, B.S., Bal, M.S. and Kaur, P. (2019). Coccidiosis in goats: Pathological observations on intestinal developmental stages and anticoccidial efficacy of amprolium. Indian J. Anim Res. 53 (02): 245-249.
  20. Le, T.H., De, N.V., Agatsuma, T., Nguyen, T.G.T., Nguyen, Q.D., McManus, D.P., Blair, D. (2008). Human fascioliasis and the presence of hybrid/introgressed forms of Fasciola in Vietnam. Int. J. Parasitol. 38(6): 725–730.
  21. Lin, Ai., Chen, M., Alasaad, S., Elsheikha, H.M., Li, J., Li, H., Lin, R., Zou, F., Zhu, X., Chen, J. (2011). Genetic characterization, species differentiation and detection of Fasciola spp. by molecular approaches. Parasitol. Vector. 4: 101. doi: 10.1186/1756-    3305-4-101.
  22. Lin, R.Q., Dong, S.J., Nie, K., Wang, C.R., Song, H.Q., Li, A.X., Huang, W.Y., Zhu, X.Q. (2007). Sequence analysis of the first internal transcribed spacer of rDNA supports the existence of the intermediate Fasciola between F. hepatica and F. gigantica in mainland China. Parasitol Res. 101(3):813–817.
  23. Luna, L. G. (1968). Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology 3rd ed. American Registry of Pathology.
  24. Moudgil, A.D., Singla, L.D. and Gupta, K. (2018). Morpho-pathological description of fatal concurrent intestinal and renal parasitism in Columba livia domestica in India. Indian J. Ani. Res. 52(7): 1063-1067.
  25. Peng, M., Ichinomiya, M., Ohtori M., Ichikawa, M., Shibahara, T., Itagaki, T. (2009). Molecular characterization of Fasciola hepatica, Fasciola gigantica and aspermic Fasciola sp. in China based on nuclear and mitochondrial DNA. Parasitol Res. 105(3): 809–815.
  26. Phiri, A. M., Phiri, I. K., Sikasunge, C.S., Chembensofu, M., Monrad, J. (2006). Comparative fluke burden and pathology in condemned and non condemned cattle livers from selected abattoirs in Zambia. Onderstepoort J. Vet Res. 73:275-281.
  27. Prasad, P.K., Tandon, V., Biswal, D. K., Goswami, M., Chatterjee, A. (2008). Molecular identification of the Indian liver fluke, Fasciola (Trematoda: Fasciolidae) based on the ribosomal internal transcribed spacer regions. Parasitol. Res. 103(6): 1247-    1255. 
  28. Prasad, P.K., Tandon, V., Biswal, D.K., Goswami, L.M. and Chatterjee A. (2009). Use of sequence motifs as barcodes and secondary structures of Internal Transcribed spacer 2 (ITS2, rDNA) for identification of the Indian liver fluke. Fasciola (Trematoda: Fasciolidae). Bioinformation. 3(7): 314-320.
  29. Raina, O. K., Jacob, S.J., Sankar, M., Bhattacharya, D., Bandyopadyay, S., Varghese, A., Chamuah, J. K. and Lalrinkima, H. (2015). Genetic characterization of Fasciola gigantica from different geographical regions of India by ribosomal DNA markers. J.Parasit Dis. 39(1):27-32.
  30. Rajkhowa, S., Bujarbaruah, K.M., Rajkhowa, C., Kapenlo, T. (2005). Incidence of intestinal parasitism in mithun (Bos frontalis). J. Vet Parasitol. 19:39-41.
  31. Sharma, R.L., Dhar, D.N and Raina, O.K. (1989). Studies on the prevalence and laboratory transmission of fascioliasis in animals in the Kashmir valley. Br Vet J. 145(1): 57-61.
  32. Soulsby, EJL (1986). Helminths, Arthropods and Protozoa of Domesticated Animals. 7th edn. ELBS, Baillere, 
  33. Tandon, V., Kar, P. K., Das, B., Sharma, B., Dorjee, J. (2005). Preliminary survey of gastrointestinal helminth infection in herbivore livestock of mountainous regions of Bhutan and Arunachal Pradesh. Zoos Print J. 20: 1867-1868.
  34. Terasaki, K., Moriyama-Gonda, N., Noda, Y. (1998). Abnormal spermatogenesis in the common liver fluke (Fasciola sp.) from Japan and Korea. J. Vet Med Sci. 60(12): 1305–1309.
  35. Varma, A.K. (1953). On Fasciola indica sp. with some observations on F. 563 hepatica and F. gigantica. J. Helminthol. 27:185–198.
  36. Wannasan, A., Khositharattanakool, P., Chaiwong, P., Piangjai, S., Uparanukraw, P., Morakote, N. (2014). Identification of Fasciola species based on mitochondrial and nuclear DNA reveals the co-existence of intermediate Fasciola and Fasciola gigantica in Thailand. Exp Parasitol. 146: 64-70.
  37. Wiedosari, E., Graydon, R., Copeman, D.B. (1991). Comparative pathological study of hepatic changes induced by Fasciola gigantica and Gigantocotyle explanatum in Javanese thin tailed Sheep. J. Comp Pathol. 105(2): 147-55. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)