Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 8 (august 2020) : 1012-1017

Molecular characterization and histopathological studies on Fasciola gigantica in Mithun (Bos frontalis)

J.K. Chamuah, D. Borkotoky, Amenti, K. Khate, S.S Jacob, Lalchamliani, O.K. Raina, M.H Khan, A. Mitra
1ICAR-National Research Centre on Mithun, Medziphema-797 106, Nagaland, India.
Cite article:- Chamuah J.K., Borkotoky D., Amenti, Khate K., Jacob S.S, Lalchamliani, Raina O.K., Khan M.H, Mitra A. (2019). Molecular characterization and histopathological studies on Fasciola gigantica in Mithun (Bos frontalis). Indian Journal of Animal Research. 54(8): 1012-1017. doi: 10.18805/ijar.B-3856.

ABSTRACT

Mitochondrial DNA sequence of the sub-unit 1 of cytochrome c oxidase (Cox1) and ribosomal DNA sequence of the first internal transcribed spacer (ITS-1) of Fasciola collected from mithun and cattle from Arunachal Pradesh, India were characterized at molecular level in order to identify the species of parasite. Based on sequence and phylogenetic analysis, the identity of the parasite was confirmed as Fasciola gigantica in mithun as well as from cattle. In order to know histopathological alteration in Fasciola infection, a histopathological study was performed on eight liver specimens, out of 110 animals studied during the period from 2010-2016. Histopathological examination of F.gigantica infected liver showed extensive fibrous connective tissue proliferation with necrosis of hepatocytes and infiltration of  polymorphonuclear cell. There was evidence of migratory  tracts of parasites with losing of normal lobular hepatic architecture. Bile duct proliferation was followed by congestion of portal vein with perivascular cuffing with surrounding degeneration of hepatocytes. The hepatocytes showed pyknosis with hyperplasia of bile duct. 

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