Molecular characterization and histopathological studies on Fasciola gigantica in Mithun (Bos frontalis)

DOI: 10.18805/ijar.B-3856    | Article Id: B-3856 | Page : 1012-1017
Citation :- Molecular characterization and histopathological studies on Fasciola gigantica in Mithun (Bos frontalis).Indian Journal Of Animal Research.2020.(54):1012-1017
J.K. Chamuah, D. Borkotoky, Amenti, K. Khate, S.S Jacob, Lalchamliani, O.K. Raina, M.H Khan and A. Mitra  drjayantavet@gmail.com
Address : ICAR-National Research Centre on Mithun, Medziphema-797 106, Nagaland, India.
Submitted Date : 10-06-2019
Accepted Date : 10-07-2019

Abstract

Mitochondrial DNA sequence of the sub-unit 1 of cytochrome c oxidase (Cox1) and ribosomal DNA sequence of the first internal transcribed spacer (ITS-1) of Fasciola collected from mithun and cattle from Arunachal Pradesh, India were characterized at molecular level in order to identify the species of parasite. Based on sequence and phylogenetic analysis, the identity of the parasite was confirmed as Fasciola gigantica in mithun as well as from cattle. In order to know histopathological alteration in Fasciola infection, a histopathological study was performed on eight liver specimens, out of 110 animals studied during the period from 2010-2016. Histopathological examination of F.gigantica infected liver showed extensive fibrous connective tissue proliferation with necrosis of hepatocytes and infiltration of  polymorphonuclear cell. There was evidence of migratory  tracts of parasites with losing of normal lobular hepatic architecture. Bile duct proliferation was followed by congestion of portal vein with perivascular cuffing with surrounding degeneration of hepatocytes. The hepatocytes showed pyknosis with hyperplasia of bile duct. 

Keywords

Fasciola gigantica Mithun Molecular identification Pathological alteration

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