Morphometric and molecular characterization of Thelohanellus catlae infecting the gills of Indian major carp catla, Catla catla (Hamilton, 1822) from West Bengal, India

DOI: 10.18805/ijar.B-3850    | Article Id: B-3850 | Page : 883-888
Citation :- Morphometric and molecular characterization of Thelohanellus catlae infecting the gills of Indian major carp catla, Catla catla (Hamilton, 1822) from West Bengal, India.Indian Journal Of Animal Research.2020.(54):883-888
Vijay Sundar Deva G, Sayani Banerjee, Debapriyo Mukherjee, B.R. Sona, K. Karuppasamy, T.J. Abraham and Gadadhar Dash vijaydeva9194@gmail.com
Address : Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Chakgaria, Kolkata-700 094, West Bengal, India.
Submitted Date : 27-05-2019
Accepted Date : 10-07-2019

Abstract

The morphometric and molecular characterization of Thelohanellus catlae a new myxozoan belonging to genus Thelohanellus Kudo, 1933 (Myxosporea:Bivalvulida) infecting the gills of Catla catla were described in this study. The infection was observed as irregular, milky whitish, cyst-like plasmodia (up to 2.0-3.0 mm in diameter) attached to the gill filaments of the host fish. Each spore has a single pyriform polar capsule. Spores measured about 20.4±0.69 (19.3-21.4) × ìm long and 11.5± 0.63 (10.7-12.4) ìm wide. The polar capsule was 12.3±0.70 (10.7- 13.9) ìm long and 10.95± 0.28 (9.7-12.9) ìm wide. Sequencing of 18S rRNA gene of the new myxozoan revealed 98% sequence homology between the 18S rRNA gene of T.catlae. Phylogenetic analysis showed T. catlae within the clade of gill infecting myxosporeans and closed together with T. catlae. The importance of myxosporeans introduction to new locations along with infected cultured host is emphasized.

Keywords

Catla catla Morphometric and molecular identification Thelohanellus catlae

References

  1. Akhmerov, A. K. (1955). Ways of the origin of Myxosporidia species of the genus Thelohanellus Kudo from Amur wild carp. In Dokl. Akad. Nauk SSSR. 105: 1129-1132.
  2. Akhmerov, A. K. (1960). Myxosporidia of fishes of the Amur River Basin. Rybnoe Khozyaistvo Vnutrikh Vodoemov Latviiskoi SSR, 5: 239-308.
  3. Anjan, M., Sayani, B., Avijit, P., Harresh, A., Raghu, R. K., Gadadhar, D., ... and Jawahar, A. T. (2014). Molecular and morphometric characterization of Thelohanellus caudatus (Myxosporea: Myxobolidae) infecting the caudal fin of Labeo rohita (Hamilton).    Protistology, 8(2).
  4. Azevedo, C., Samuel, N., Saveia, A. P., Delgado, F., and Casal, G. (2011). Light and electron microscopical data on the spores of Thelohanellus rhabdalestus n. sp.(Myxozoa: Myxosporea), a parasite of a freshwater fish from the Kwanza River, Angola. Systematic parasitology, 78(1): 19-25.
  5. Basu, S., and Haldar, D. P. (1999). Thelohanellus bifurcata n. sp. a new species of the genus Thelohanellus from hybrid carps and checklist of the species of the genus described from Indian fishes. Proceedings of the Zoological Society Calcutta, 52(1): 115-124.
  6. Basu, S., and Haldar, D. P. (2004). Description of three new myxosporean species (Myxozoa: Myxosporea: Bivalvulida) of the genera Myxobilatus Davis, 1944 and Myxobolus Butschli, 1882. Acta Protozoologica, 43(4): 337-344.
  7. Basu, S., Modak, B. K., and Haldar, D. P. (2006). Synopsis of the Indian species of the genus Thelohanellus Kudo, 1933 along with description of Thelohanellus disporomorphus sp. n. J Parasitol Appl Anim Biol, 15(1-2): 81-94.
  8. Chakravarty, M., and Basu, M. S. (1948). Observations on some myxosporidians parasitic in fishes, with an account of nuclear cycles in one of them. In Proceedings of the Zoological Society Calcutta. 1: 23-33.
  9. Chandra, K. J., Begum, A. A., Ahmed, G. U., and Wootten, R. (1996). Infection of Myxosporean ectoparasites of juvenile carps in nurseries of Mymensingh, Bangladesh. Bangla Journal of aquaculture. 18: 39-44..
  10. Egusa, S., and Nakajima, K. (1981). A new myxozoa Thelohanellus kitauei, the cause of intestinal giant cystic disease of carp. Fish Pathology, 15(3-4): 213-218.
  11. Eszterbauer, E. (2004). Genetic relationship among gill-infecting Myxobolus species (Myxosporea) of cyprinids: molecular evidence of importance of tissue-specificity. Diseases of Aquatic Organisms, 58(1): 35-40.
  12. Eszterbauer, E., Benkó, M., Dán, Á. and Molnár, K. (2001). Identification of fish-parasitic Myxobolus (Myxosporea) species using a combined PCR-RFLP method. Diseases of Aquatic Organisms, 44(1): 35-39.
  13. Eszterbauer, E., MáriaBenkó, Dán1, A., Molnár, K., (2001). Identification of fish- parasiticMyxobolus(Myxosporea) species using a combined PCR-RFLP method, Diseases of Aquatic Organisms. 44: 35–39.
  14. Fiala, I. (2006). The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. International Journal for Parasitology, 36(14): 1521-1534.
  15. Felsenstein, J., (1985). Phylogenies and the comparative method. The American Naturalist, 125(1):1-15.
  16. Hossain, M. A., Banu, A. N. H., and Khan, M. H. (1994). Prevalence of ectoparasite in carp nursery operation of greater Mymensingh.    Progressive Agriculture, 5: 39-44.
  17. Hossain, M. A., Banu, A. N. H., Khan, M. H., and Sultana, N. (1994). Bacterial microflora isolated from carp and catfish fry and their sensitivity to some antibiotics [in Bangladesh]. Bangladesh Journal of Microbiology (Bangladesh).
  18. Kabre, G. B., Sakiti, N. G., Marques, A., and Sawadogo, L. (2002). Thelohanellus bicornei n. sp. Myxosporidie (Myxosporea, Bivalvulida) a gill parasite of Labeo coubie Rüppel, 1832 (Osteichthyen, Cyprinidae) from Burkina Faso, West Africa. Parasite (Paris, France), 9(3), 219-223.
  19. Kaur, H., Shoaib, A. D., and Singh, R. (2012). A report on three Myxozoan parasites causing gill Myxoboliosis in Aquaculture fishes in Punjab (India). Trends in Parasitology, 1(3): 1-6.
  20. Kaur, H., Singh, R., Katoch, A., Attri, R., Dar, S. A. and Gupta, A. (2017). Species diversity of the genus Thelohanellus Kudo, 1933 (Myxozoa: Bivalvulida) parasitizing fishes in Indian subcontinent. Journal of Parasitic Diseases, 41(2): 305-312.
  21. Kent, M. L., Andree, K. B., Bartholomew, J. L., EL MATBOULI, M. A. N. S. O. U. R., Desser, S. S., Devlin, R. H., ... and Hallett, S. L. (2001). Recent advances in our knowledge of the Myxozoa. Journal of Eukaryotic Microbiology, 48(4): 395-413.
  22. Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16(2): 111-120.
  23. KuDO, R. (1915). On a new Myxosporidian from a carp. Journal of Zoology, 27: 517-523.
  24. Kudo, R. (1933). A taxonomic consideration of Myxosporidia. Transactions of the American Microscopical Society, 52(3): 195-216.
  25. Kundu, T. K. and Haldar, D. P. (1981). Studies in Thelohanellus jiroveci sp. n., a new myxosporidian parasitic in a common teleost fish. Vestnik Ceskoslovenske spolecnosti zoologicke..
  26. Lewisch, E., Soliman, H., Schmidt, P. and El-Matbouli, M. (2015). Morphological and molecular characterization of Thelohanellus hoffmanni sp. nov.(Myxozoa) infecting goldfish Carassius auratus auratus. Diseases of Aquatic Organisms, 115(1): 37-46.
  27. Lightner, D. V. (1993). Diseases of cultured penaeid shrimp. CRC handbook of mariculture, 1: 393-486.
  28. Lom, J., and Arthur, J. R. (1989). A guideline for the preparation of species descriptions in Myxosporea. Journal of Fish Diseases, 12(2): 151-156.
  29. Molnár, K. (1994). Comments on the host, organ and tissue specificity of fish myxosporeans and on the types of their intrapiscine development. Parasitol Hung, 27: 5-20.
  30. Moshu, A., and Molnar, K. (1997). Thelohanellus (Myxozoa: Myxosporea) infection of the scales in the European wild carp Cyprinus carpio carpio. Diseases of Aquatic Organisms, 28(2): 115-123.
  31. Pagarkar, A. U., and Das, M. (1993). Two new species of myxozoa, Thelohanellus caudatus n. sp. and Myxobolus serrata n. sp. from cultural carps. J Inland Fish Soc India, 25(1): 30-35. 
  32. Roberts, R. J. (2012). Fish pathology. John Wiley and Sons.
  33. Shin, S., Kim, J., Choresca, C., Han, J., Jun, J., and Park, S. (2012). Molecular identification and phylogenetic characterisation of Thelohanellus kitauei. Acta Veterinaria Hungarica, 61(1): 30-35.
  34. Singh, R., and Kaur, H. (2012). Myxosporean species of the genus Thelohanellus Kudo, 1933 (Myxozoa: Myxosporea: Bivalvulida) from freshwater fishes of Punjab wetlands, India. Protistology, 7(4): 209-217.
  35. Southwell, T., and Prashad, B. (1918). Notes from the Bengal Fisheries Laboratory: Parasites of Indian Fishes with a Note on Carcinoma in the Climbing Perch.
  36. Srivastava, C.B. (1980). Estimation of helminthic infections. Procedings of the Workshop on Techniques in Parasitology, Zoological Survey of India, Kolkata, 29-31.
  37. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular biology and evolution, 28(10): 2731-2739.
  38. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., and Higgins, D. G. (1997). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic acids research, 25(24): 4876-4882.
  39. Zhang, J. Y., Gu, Z. M., Kalavati, C., Eiras, J. C., Liu, Y., Guo, Q. Y., and Molnár, K. (2013). Synopsis of the species of Thelohanellus Kudo, 1933 (Myxozoa: Myxosporea: Bivalvulida). Systematic parasitology, 86(3): 235-256.
  40. Zhu, Y. T., Lu, H. D., and Cai, S. J. (2012). Redescription of Thelohanellus wuhanensis Xiao et Chen (Myxozoa, Myxosporea) infecting allogynogenetic crucian carp (Carassius auratus gibelio) and phylogenetic analysis based on 18S rDNA sequence. Acta Zootaxonomica Sinica, 37: 681-686.

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