Heparin Binding Proteins of Black Bengal Buck Semen and Their Correlation with Sperm Characters and Freezability

DOI: 10.18805/ijar.B-3849    | Article Id: B-3849 | Page : 829-834
Citation :- Heparin Binding Proteins of Black Bengal Buck Semen and Their Correlation with Sperm Characters and Freezability.Indian Journal Of Animal Research.2020.(54):829-834
M. Karunakaran, Vivek C. Gajare, Ajoy Mandal, Mohan Mondal, S.K. Das, S. Rai and M.K. Ghosh  drmkarunakaran@gmail.com
Address : ICAR- National Dairy Research Institute, Eastern Regional Station, Kalyani-741 235, West Bengal, India.
Submitted Date : 25-05-2019
Accepted Date : 15-10-2019


This experiment was conducted to study the electrophoretic characters of heparin binding proteins (HBP) of Black Bengal buck semen and their correlation with sperm characters and cryo-survivability. Semen ejaculates (n=20/buck) were collected from nine bucks and in vitro sperm characters were evaluated at collection, after equilibration and after freeze - thawing. HBP were isolated through heparin column and discontinuous Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was performed to assess molecular weight. Significant difference (P<0.01) were observed among the bucks in sperm characters and freezability. Eight protein bands of 17 to 180 kDa in seminal plasma and 7 bands in sperm were found. 180 -136 kDa HBP of seminal plasma and 134-101 kDa HBP of sperm had showed high correlation with in vitro sperm characters. Further studies on identification of these proteins and their correlation with in vivo pregnancy are needed to find their role as marker for buck selection. 


Buck Freezability Heparin binding proteins In vitro characters Semen


  1. Apu, A.S., Khandoker, M.A.M.Y., Husain, S.S., Rahman, S.M.H.A. and Notter, R.D. (2008). Investigation of seminal attributes and fertility of Black Bengal goat. Bangladesh Journal of Animal Science. 37: 17-24. 
  2. Arangasamy, A., Singh, L.P., Ahmed, N., Ansari, M.R. and Ram, G.C. (2005). Isolation and characterization of heparin and gelatin binding buffalo seminal plasma proteins and their effect on cauda epididymal spermatozoa. Animal Reproduction. Science. 90: 243–254. 
  3. Asadpour, R., Alavi-Shoushtari, S.M., Rezaii, S.A. and Ansari, M.K. (2007). SDS-polyacrylamide gel electrophoresis of buffalo bulls seminal plasma proteins and their relation with semen freezability. Animal Reproduction Science. 102(3-4): 308-313. 
  4. Ax, R.L. (2004). A new test screens bulls for a protein that enables them to settle more cows earlier. Western Cowman. 7: 28-32. 
  5. Bellin, M. E., Oyarzo, J. N., Hawkins, H.E., Zhang, H., Smith, R.G., Forrest, D.W., Sprott, L.R. and Ax, R.L. (1998). Fertility- associated antigen on bull sperm indicates fertility potential. Journal of Animal Science. 76: 2032-2039. 
  6. Deori, S., Deka, B., Biswas, R., Nahardeka, N., Arangasamy, A., Bhuyan, D., Kalita, D., Borah, R. and Phookan, A. (2018). Characteristics and freezability of Assam Hill goat semen. Indian Journal of Animal Research. 52 (1): 25-28. 
  7. Khandoker, M.A.M.Y., Apu, A.S., Husain, S.S. and Notter, D.R. (2011). A baseline survey on the availability of Black Bengal breeding bucks in different districts of Bangladesh. Journal of the Bangladesh Agricultural University. 9(1): 91-96. 
  8. Karunakaran, M. (2011). Evaluation of bull semen quality in-relation to fertility associated proteins, in vitro sperm characters and lipid peroxidation. Thesis submitted to TANUVAS, Chennai. 
  9. Karunakaran, M., Devanathan, T.G., Jawahar, T.P., Manimaran, K., Chitra, A., Dhali, A. and Selvaraju, S. (2012). Electrophoretic profile of bull sperm membrane proteins as a tool for selection of breeding bulls. Indian Journal of Animal Science. 82: 1303–13 05. 
  10. Karunakaran, M. and Devanathan T.G. (2016). Evaluation of bull semen for fertility-associated protein, in vitro characters and fertility. Journal of Applied Animal Research. 45(1): 136- 144. 
  11. Karunakaran, M., Konyak, P., Mandal, A., Mondal, M., Bhakat, C., Mandal, D.K., Das, S.K. and Ghosh, M.K. (2017). Chemically well-defined extender for preservation of Black Bengal buck semen. Research and. Reviews: Journal of Dairy Science and Technology. 6(2): 7-10. 
  12. Karunakaran, M., Konyak, P., Mandal, A., Mondal, M., Bhakat, C., Saroj, R., Ghosh, M.K. and Behera, R. (2019). Effect of trehalose- an impermeant cryoprotectant on cryopreservation of Black Bengal buck semen. Indian Journal of Animal Research. 53(1): 37-40. 
  13. Konyak, P., Mandal, A., Mondal, M., Bhakat, C., Das, S.K., Saroj, R., Ghosh, M.K. and Karunakaran, M. (2018). Preservation of Black Bengal buck semen in soybean lecithin based chemically defined extender. Indian Journal of Animal Research. 52(8): 1151-1154. 
  14. Krishnan, G., Thangvel, A., Loganathasamy, K., Veerapandian, C., Kumarasamy, P. and Karunakaran, M. (2016). The presence of heparin    binding proteins and their impact on semen quality of Holstein Friesian bulls. Indian Journal of Animal Science. 86(4):392-396. 
  15. Laemmli, V.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 660- 685. 
  16. Manaskova, P., Liberda, J.V., Ticha, M. and Jonakova, V. (2002). Isolation of non-heparin-binding and heparin-binding proteins of boar prostate. Journal of Chromatography. 770: 137- 143. 
  17. McCauley, T.C., Zhang, H.M., Bellin, M.E. and Ax, R.X. (2001).Identification of a heparin- binding protein in bovine seminal fluid as tissue inhibitor of metalloproteinases-2. Molecular Reproduction and Development. 58: 336-341. 
  18. Miller, D.J., Winer, M.A. and Ax, R.L. (1990). Heparin binding proteins from seminal plasma bind to bovine spermatozoa and modulate capacitaion by heparin. Biology of Reproduction. 42: 899-915. 
  19. Nandi, D., Roy, S., Bera, S., Kesh, S.S. and Samanta, A.K. (2011). The rearing system of Black Bengal goat and their farmers in West Bengal, India. Veterinary World. 4(6): 254-257. 
  20. Nass, S.J., Miller, D.J., Winner, M.A. and Ax, R.L. (1990). Male accessory sex glands produce heparin-binding proteins that bind to cauda epididymal spermatozoa and are testosterone dependant. Molecular Reproduction and Development. 25: 237- 246. 
  21. Singh, A., Brar, P. and Cheema, R. (2016). Heparin binding proteins and their relationship with vital sperm function tests vis-à-vis fertility of buffalo bull semen. Asian Pacific Journal of Reproduction. 5(5): 419-427. 
  22. Sprott, L.R., Harris, M.D., Forrest, D.W., Young, J., Zhang, H.M., Oyarzo, J.N., Bellin, M.E. and Ax, R.L. (2000). Artificial insemination outcomes in beef females using bovine sperm with a detectable fertility-associated antigen. Journal of Animal Science. 78:795-798. 
  23. Teixeira, D., Cavada, B., Sampaio, A., Havt, A., Bloch Jr.C., Prates, M., Moreno, F., Santos, E., et al (2002). Isolation and partial characterisation of a protein from buck seminal plasma (Capra hircus), homologous to spermadhesins. Protein and Peptide Letters. 9(4): 331-335. 
  24. Therien, I., Moreau, R. and Manjunath, P. (1998). Major proteins of bovine seminal plasma and high-density lipoproteins induce cholesterol efflux from epididymal sperm. Biology of Reproduction. 59: 769-776. 
  25. Yue, W., Shi, L., Bai, Z., Ren, Y. and Zhoa, Y. (2009). Sodium dodecyl sulfate (SDS) – polyacrylamide gel electrophoresis of ram seminal plasma proteins and their correlation with semen characteristics. Animal Reproduction Science. 116: 386– 91. 

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