Detection of Norfloxacin in Muscle, Liver and Kidney of Broiler Chicken

DOI: 10.18805/ijar.B-3839    | Article Id: B-3839 | Page : 739-743
Citation :- Detection of Norfloxacin in Muscle, Liver and Kidney of Broiler Chicken.Indian Journal Of Animal Research.2020.(54):739-743
N.S. Meena, Y.P. Sahni, K. Shrman, Ankit Kumar Singh, Akshay Kumar nmeena2126@gmail.com
Address : Director Research Services, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 004, Madhya Pradesh, India.
Submitted Date : 10-05-2019
Accepted Date : 9-08-2019

Abstract

The present study was undertaken to determine the residues of norfloxacin in muscle, liver and kidney of broiler chicken. On the basis of surveillance study, commonly used antibiotic norfloxacin was selected for quantifying the concentration in broiler chicken meat samples. The HPLC system was equipped with photodiode array and C18 hypersil column. The mobile phase consisting of 1 ml Ortho phosphoric acid (85 per cent v/v), 100 ml water HPLC grade with acitonitrile HPLC grade, 87:12 (v/v) then pH was adjusted to 2.0 with triethylamine HPLC Grade. The results indicated that out of 180 samples analysed, 3 liver samples of target stations, viz. T3, T6 and T10 were found positive for norfloxacin residues with concentrations of 2.00, 2.60 and 2.31 µg/kg, respectively. Samples collected from other target stations did not show residual concentration of norfloxacin in muscle, liver and kidney samples.

Keywords

Antibiotic Hypersil column Norfloxacin Photodiode array

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