Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 54 issue 6 (june 2020) : 739-743

Detection of Norfloxacin in Muscle, Liver and Kidney of Broiler Chicken

N.S. Meena, Y.P. Sahni, K. Shrman, Ankit Kumar Singh, Akshay Kumar
1Director Research Services, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 004, Madhya Pradesh, India.
Cite article:- Meena N.S., Sahni Y.P., Shrman K., Singh Kumar Ankit, Kumar Akshay (2019). Detection of Norfloxacin in Muscle, Liver and Kidney of Broiler Chicken. Indian Journal of Animal Research. 54(6): 739-743. doi: 10.18805/ijar.B-3839.
The present study was undertaken to determine the residues of norfloxacin in muscle, liver and kidney of broiler chicken. On the basis of surveillance study, commonly used antibiotic norfloxacin was selected for quantifying the concentration in broiler chicken meat samples. The HPLC system was equipped with photodiode array and C18 hypersil column. The mobile phase consisting of 1 ml Ortho phosphoric acid (85 per cent v/v), 100 ml water HPLC grade with acitonitrile HPLC grade, 87:12 (v/v) then pH was adjusted to 2.0 with triethylamine HPLC Grade. The results indicated that out of 180 samples analysed, 3 liver samples of target stations, viz. T3, T6 and T10 were found positive for norfloxacin residues with concentrations of 2.00, 2.60 and 2.31 µg/kg, respectively. Samples collected from other target stations did not show residual concentration of norfloxacin in muscle, liver and kidney samples.
  1. Ammar, A.M., Abd El-Aziz, N.K., Hanafy, M.S., Ibrahim, O. A. (2016). Serotypes profile of avian salmonellae and estimation of antibiotic residues in chicken muscles using high-performance liquid chromatography. Advances in Environmental Biology. 10(7): 173-179. 
  2. Anderson, S.A., Woo, R.W.Y., Abd. O. and Crawford, L.M. (2001). Risk assessment of the impact on human health of resistant Campylobacter jejuni from fluoroquinolone use in beef cattle. Food Control. 12:13-25.
  3. Hassouan, M.K., Ballesteros, O., Taoufiki, J., Vilchez, J.L., Cabrera – Aguilera, M., Navalon, A., (2007). Multiresidue determination of quinolone antibacterials in eggs of laying hens by liquid chromatography with fluorescence detection. Journal of Chromatography B. 852: 625-630, 
  4. Hee-Jung, C., Abd, A. M., Goudah, A., Sung, G. M., Hee, Y., Seo, D. C., Kim, J. S., et al (2011). Monitoring of fluoroquinolone residual levels in chicken eggs by microbiological assay and confirmation by liquid chromatography. Biomedical Chromatography.    22: 92-99.
  5. Heshmati, A., Kamkar, A., Salaramoli, J., Hassan, J., Jahed, G.H. (2015). Effect of deep-frying processing on tylosin residue in meat. Iranian Journal of Nutrition Sciences and Food Technology. 12: 61-71.
  6. Kowalski, C., Rolinski, Z., Slawik, T. and Glod, B. K. (2007). Determination of norfloxacin in chicken tissues by HPLC with fluorescence detection. Journal of Liquid Chromatography and Related Technologies. 28: 121-135. 
  7. Kozarova, L., Mate, D. and Cabadaj, R. (2001) Methods for the determination of sulfonamides residues in milk and animal n products In: Proceeding of Lectures and Posters of the International Conference HygienaAlimentorium XXIII. StrbskePleso, High Tatras, SR. p159-163.
  8. Mustafa, A.Z. and Ghamdi, A.M. (2001). Use of norfloxacin in poultry production in the eastern province of Soudi Arabia and its possible impact on public health. International Journal of Environmental Health Research. 10: 291-299.
  9. Oliphant, C.M. and Green, G.M. (2002). Quinolones: a comprehensive review. Clinical Pharmacology. 65: 13-18.
  10. Pena, A., Silva, L.J.G., Pereira, A., Meisel, L. and Lino, C.M. (2010). Determination of fluoroquinolone residues in poultry muscle in Portugal.Analytical and Bioanalytical Chemistry. 397: 2615-2621 
  11. Prescott, J.F. and Baggot, J.D. (1993). Fluoroquinolones. In Antimicorbial Therapy in Veterinary Medicine 2nd edn (J.F. Prescott ad J.D. Baggot, eds), pp.252-262.Iowa State University Press. Iowa: 

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