Characterization of indigenous silkworm (Bombyx mori L.) breeds on the basis of morphological traits

DOI: 10.18805/ijar.B-3838    | Article Id: B-3838 | Page : 685-695
Citation :- Characterization of indigenous silkworm (Bombyx mori L.) breeds on the basis of morphological traits.Indian Journal Of Animal Research.2020.(54):685-695
Rubia Bukhari, R.K. Bali and Rashad Hussain Shah rubiabukhari@gmail.com
Address : Division of Sericulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Main Campus, Chatha, Jammu-180 009, Jammu and Kahmir, India.
Submitted Date : 4-05-2019
Accepted Date : 11-07-2019

Abstract

The morphological characterization among domesticated insect like silkworm (Bombyx mori L.) is an important aspect for selection of suitable parents for breeding programme. The present study was conducted on twenty bivoltine silkworm breeds to estimate the extent relationship by morphological characterization. Eighteen morphological traits both qualitative and quantitative viz., egg shape, chorion colour, colour of the newly hatched larva, larval marking, cocoon colour, cocoon shape, pupa colour and shape, colour of male and female moth, fecundity, hatching, larva weight 5th day, –th instar duration, total larval period, percentage of malformed cocoons, pupation rate, single cocoon weight, single shell weight and shell percent were studied and data revealed significant variability among the breeds. Maximum Euclidean distance was observed between NB4D2 and CSR19 (163.25) followed by CSR19 and UDHEY-5 (145.13) and minimum between UDHEY-1 and UDHEY-3 (4.20). The dendrogram of phenotypic data grouped all breeds in two clusters; Cluster A and B. Cluster A was sub divided into two sub-clusters cluster AI and cluster A II. Cluster A I contained eight breeds and Cluster AII eleven breeds. Cluster B comprised of one breed CSR19. In PCA the genetic divergence was maximum between NB4D2 and CSR19, whereas it was minimum in UDHEY-7 and ND2. PCoA results depicted CSR19 and SPO having greater divergence, whereas it was minimum between UDHEY-7 and ND2. On the basis of Euclidean distance metric the performance of the breeds; CSR19, NB4D2, PO3, UDHEY-8, SPO, UDHEY-4, ND2, CC1, UDHEY-2, UDHEY-5, UDHEY-7, UDHEY-6 have been identified as promising breeds and are recommended for further breeding to boost bivoltine silk production. 

Keywords

Bivoltine breeds Bombyx mori L. Characterization Coefficient of correlation Dendrogram Morphology PCA PCoA Yield

References

  1. Awasthi, A. K., Kar., P. K., Srivastava, P. P., Rawat, Vijayan K. and Pradeep, A. R. (2008). Molecular evaluation of bivoltine, polyvoltine and mutant silkworm (Bombyx mori L.) with RAPD, ISSR and RFLPSTS markers. Indian Journal of Biotechnology. 7: 188-94. 
  2. Basavaraja, H. K., Ashwath, S. K., Suresh Kumar, N., Mal Reddy, N. and Kalpana, G. V. (2005). Silkworm breeding and genetics. Central Silk Board Publication Bangalore. 409-439.
  3. Basavaraja, H. K., Suresh, K. Reddy, N. and Datta, R. K. (1998). New Approaches to Bivoltine Silkworm Breeding. Oxford: IBH Publishing, 131-140. 
  4. Bojan, C., Marghitas, L. A., Dezmirean, D. and Orsolya T. (2007). Qualitative characters study for silkworm hybrids. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-napoca Agriculture. 64: 219-223.
  5. Chatterjee, S. N., Rao, P. P. M., Jayaswal, K. P., Singh, R. and Datta, R. K. (1993) a. Genetic variability in mulberry silkworm, Bombyx mori L. breeds with low silk yield. Indian Journal of Sericulture. 32(1): 69-86.
  6. Chatterjee, S. N. and Pradeep, A. R. (2003). Molecular markers (RAPD) associated with growth, yield and origin of the silkworm, Bombyx mori L. in India. Genetika. 39: 1612-1624. 
  7. Chanda, S., Saha, L. M., Das, N. K., Kar, N. B. and Bindroo, B. B. (2013). Correlation among shell percent, cocoon yield and reeling parameters of multi x bi cocoons under different agro-climatic conditions of West Bengal, India. International Journal of Industrial Entomology. 26(2): 74-80.
  8. Chandrakanth, N., Moorthy, S. M., Rekha, M. and Sivaprasad, V. (2016). Stability and path analysis for yield and related traits in silkworm, (Bombyx mori L.) reared under stress conditions. Genetika. 48(1): 271-284.
  9. Dandin, S. B., Jayant, J. and Giridhar, K. (2003). Handbook of Sericulture Technologies. Central Silk Board, Bangalore.
  10. Daniel, M., Maly, M., Danielova, V., Kriz, B. and Nuttall, P. (2015). Abiotic predictors and annual seasonal dynamics of Ixodesricinus, the major disease vector of Central Europe. Parasites and Vectors. 8: 1-12.
  11. Furdui, E.M., Marghitas, L.A., Dezmirean, D., Mihai, C.M., Bobis, O and Pasca, I. (2010). Comparative study of biological characters of larvae, crude and dried cocoon in 7 races of Silkworm Bombyx mori L. raised in Transylvania area. Scientific Papers: Animal Science And Biotechnologies. 43(1): 490-493.
  12. Gurjar, T. S., Siddhapara, M. R. and Patel, A. H. (2018). Rearing performance of various races of mulberry silkworm, Bombyx mori L. International Journal of Agriculture Sciences. 10(13): 6625-6627.
  13. Jolly, M. S. (1983) Silkworm breeding in Indian. In National seminar on Silk Resources and Development, Bangalore, March, 10th–13th:1-9.
  14. Kamili, A. S., Masoodi, M. A., Sofi, A. M., Malik, G. N. and Malik, F. A. (1996). Nature and degree of association between various economic attributes of bivoltine silkworm, Bombyx mori L. Oriental Science. 1(2): 105-108.
  15. Kumaresan, P., Sinha, R. K., Sahni, N. K. and Sekar, S. (2000). Genetic variability and selection indices for economic quantitative traits of multivoltine mulberry silkworm, Bombyx mori L. genotypes. Sericologia. 40(4): 595-605.
  16. Mano, Y., Nirmal Kumar, S., Basavaraja, H. K., Mai Reddy, N. and Datta, R. K. (1993). A new method to select promising silkworm breeds/combinations. Indian Silk. 31(10): 53-58.
  17. Masarat, B., Afifa. S. K and Sharma, R. K. (2014). Assessment of variability between growth and yield attributes of bivoltine silkworm Bombyx mori L. under temperate climatic conditions. International Journal of Advanced Biological Research. 4(3): 374-    379.
  18. Moorthy, S. M. and Chandrakanth, N. (2015). Analysis of phenotypic diversity and Protein polymorphism in some Silkworm breeds of Bombyx mori L. Munis Entomology and. Zoology. 10(2): 478-485.
  19. Nezhad, M. S., S. Z. Mirhosseini, S. Gharahveysi, M. Mavvajpour, and A. R. Seidavi. (2010). Investigation on intera-speciûc biodiversity of 51 peanut cocoon strains of Iran silkworm (Bombyx mori L.) germplasm based on reproductive traits. Biotechnology. 9: 149-156.
  20. Pal, N. B. and Moorthy, S. M. (2011). Assessment of variability in larval and cocoon traits in some genotypes of bivoltine silkworm, Bombyx mori L, International Journal of Research in Biological Sciences. 1(4): 59-65.
  21. Rathore, M. S., Srinivasulu, Y., Kour, R., Anil Dhar and Sahaf, K. A. (2012). Soil carbon sequestration through mulberry based agro-    forestry system in temperate region. Current Science. 103(6): 614-615. 
  22. Rathore, M. S., Srinivasulu,Y., Kour, R., Darzi, G. M., Anil Dhar and Khan, M. A. (2011). Integrated nutrient management in mulberry under temperate conditions. European Journal of Biological Sciences. 3(4): 105-111.
  23. Ram, K., Bali, R. K. and Koul, A. (2003). Seasonal evaluation of various cross combinations in bivoltine silkworm Bombyx mori L. Journal of Research Sher- e- Kashmir University of Agricultural Sciences and Technology- Jammu. 2(2): 169-177.
  24. Ram, K., Bali, R. K. and Koul, A. (2006). Identification of bivoltine breeds and their hybrids suitable for subtropical climate of Jammu. Geobios. 33(4): 265-270.
  25. Sajgotra, M. and Bali, R. (2016) a. Evaluation and identification of potential bivoltine silkworm hybrids of Bombyx mori L. Indian Journal of Ecology. 43(1):396-399.
  26. Sajgotra, M. and Bali, R. (2016) b. Expression of heterosis in bivoltine hybrids of Bombyx mori L. for silk yield contributing traits under sub tropical conditions. International Journal of Bio- resource and Stress Management. 7(2): 258-262.
  27. Sajgotra, M., Bali, R. K. and Sharma, R. (2017). Heterosis studies on thermotolerant hybrids of bivoltine silkworm (Bombyx mori L). Journal of Pharmacognosy and Phytochemistry. 6(5): 921-928.
  28. Singh, A., Sharma, K. and Sharma, B. (2010). Low temperature induced alterations in certain biochemical constituents of 5th instar larvae of Philosamia ricini (Lepidoptera: Saturniidae). Insect Physiology. 2: 12-16.
  29. Sonwalkar, T N. (2001). Hand Book of Silk Technology. New Age International Private Limited, New Delhi. 14-21.
  30. Tazima, Y. (1978). The Silkworm: An Important Laboratory Tool. Kodansha Scientific Books, Tamil Nadu.
  31. Ullah, S. R and Narasimahanna M N. (1978). Handbook of practical sericulture. Central silk Board, Bombycidae. 208. 

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