DNA Barcoding and Biometric Investigation on the Invasive Oreochromis niloticus (Linnaeus, 1758) from the River Yamuna of Uttar Pradesh

DOI: 10.18805/ijar.B-3833    | Article Id: B-3833 | Page : 856-863
Citation :- DNA Barcoding and Biometric Investigation on the Invasive Oreochromis niloticus (Linnaeus, 1758) from the River Yamuna of Uttar Pradesh.Indian Journal Of Animal Research.2020.(54):856-863
Absar Alam, Narinder Kumar Chadha, Annam-Pavan Kumar, Sushant Kumar Chakraborty, KripaL Datt Joshi, Paromita Banerjee Sawant, Shyamal Chndra Sukla Das, Jeetendra Kumar, Tarkeshwar Kumar Absar.Alam@icar.gov.in
Address : Regional Centre, ICAR-Central Inland Fisheries Research Institute, Allahabad-211 002, Uttar Pradesh, India.
Submitted Date : 23-04-2019
Accepted Date : 27-12-2019

Abstract

Oreochromis niloticus (Linnaeus, 1758), an invasive fish species is rapidly spreading in the Gangetic river system. The aim of the study was to identify and confirm its identity at species level by establishing a barcoding reference datasets in the river Yamuna and then to investigate its morphometric, meristic traits and length-weight relationship of O. niloticus from the Allahabad water of the river Yamuna by the examination of 341 fish specimens collected during October 2011 to September 2012. The taxonomic status of O. niloticus (Linnaeus, 1758), in the river Yamuna was assessed using DNA barcode marker COI gene sequences, have clearly identified the O. niloticus species with 100% similarity value with public database. The length-weight relationships were established as W = 0.029486L2.881638 and W = 0.058499L2.661735 for male and female. Differential growth in length-weight between male and female of O. niloticus were significant (p < 0.05), with a greater slope (b) value for male (2.88) than female (2.66). The t statistics estimated for the regression coefficients (b) were significant at 5% level of significance (p < 0.05), indicating an allometric growth pattern. The correlation was highest for the standard length and the total length (0.9878) and minimum for caudal peduncle length and total length (0.8022). Fin formula based on the meristic studies can be written as B3, D 15-19/11-15, P14, V 1/5, A 3/8-11 and C16-22. 

Keywords

Allometric Fin Formula Growth Nile Tilapia Regression coefficient

References

  1. Alam, A., Chadha, N.K., Joshi, K.D., Chakraborty, S.K., Sawant, P.B., Kumar, T., Sharma, A.P. (2015a). Maturation profile and fecundity of the exotic Oreochromis niloticus in the River Yamuna, India. Journal of Environmental Biology. 36(4): 927-931.
  2. Alam, A., Chadha, N.K., Joshi, K.D., Chakraborty, S.K., Sawant, P.B., Kumar, T., Srivastava, K., Das, S.C.S. Sharma, A.P. (2015b) Food and feeding ecology of the non-native Nile Tilapia Oreochromis niloticus (Linnaeus, 1758) in the River Yamuna, India. Proceedings of National Academy of Sciences, India Section. B Biological Sciences, 85(1): 167-174. 
  3. Arruda, B.C.C., Beasley, R.C., Vallinoto, M., Marques-Silva, S.N., Tagliaro, H.C. (2009). Significant genetic differentiation among populations of Anomalocardia brasiliana (Gmelin, 1791): a bivalve with planktonic larval dispersion. Genetics and Molecular Biology. 32: 423-430.
  4. Bernard, E., Bankole, N.O., Akande, G.R., Adeyemi, S., Ayo-Olalusi, C.I. (2010). Organoleptic characteristics, length-weight relationshiphs and condition factor of Oreochromis niloticus at Idah L.G.A in River Egah of Kogi State, Nigeria. Internet Journal of Food Safety. 12: 62-70.
  5. Beverton, R.J.H. and Holt, S.J. (1957). On the dynamics of exploited fish populations. Fish. Invest. London, Series 2, 19. 533 pp.
  6. Bhattacharya, D. and Acharya, P. (1984). A note on the length-weight relationship of Polynemus heptadctylus. Geobios. 3: 62-64.
  7. Borah, S., Bhattacharjya, B.K., Saud, B.J., Yadav, A.K., Debnath, D., Yengkokpam, S., et al. (2017). Length-weight relationship of six indigenous fish species from Deepor beel, a Ramsar site in Assam. India. Journal of Applied Ichthyology. 33(3): 655–657.
  8. Costa, J.L., De Almedia, P.R., Costa, M.J. (2003). A morphometric and meristic investigation of Lusitanian toadfish Halobat rachus didactylus (Blochand Schneider, 1801): Evidence of population fragmentation on Portuguese coast. Scienta. Marina. 67: 219-231.
  9. Doherty, D. and McCarthy, T.K. (2004). Morphometric and meristic characteristics analyses of two Western Irish populations of Arctic Char, Salvelinu salpinus (L.). Proceedings of the Royal Irish Academy. 104B (1): 75-85.
  10. Froese, R. (2006). Cube law, condition factor and weight length relationships: history, meta analysis and recommendations. Journal of Applied Ichthyology. 22: 241–253. 
  11. Grammer, G.L., Slack, W.T., Peterson, M.S., Dugo, M.A. (2012). Nile tilapia Oreochromis niloticus (Linnaeus, 1758) establishment in temperate Mississippi, USA: multi-year survival confirmed by otolith ages. Aquatic Invasion. 7(3): 367–376.
  12. Hanan, B.A. (2018) Molecular identification studies on Oestrus ovis L. (Diptera: Oestridae) larvae infested sheep in Jazan region, Saudi Arabia. Indian Journal of Animal Research. 52: 105-110.
  13. Hebert, P.D., Cywinska, A., Ball, S.L., deWaard, J.R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society, London: Biological Sciences. 270: 313-322.
  14. Jhingran, V.G. (1991). Fish and Fisheries of India. Hindustan Publishing Corporation, New Delhi, 727 pp.
  15. Joshi, K.D., Alam, A., Jha, D.N., Srivastava, K., Srivastava, S.K., Kumar, V and Sharma, A.P. (2017). Studies on ecology, fish diversity and fisheries of Ken-Betwa Rivers (India): Impact assessment of proposed inter-linking. Aquatic Ecosystem Health and Management Society. 20(1-2): 71-85.
  16. Joshi, K.D., Alam, A., Jha, D.N., Srivastava, S.K. Kumar, V. (2016). Fish diversity, composition and invasion of exotic fishes in river Yamuna under altered water quality conditions. Indian Journal of Animal Sciences. 86(8): 957–963.
  17. Joshi, K.D., Jha, D. N., Alam, A., Das, S.C.S., Srivastava, S.K., Kumar, V. (2014). Massive Invasion of resilient exotic fishes in the river Ganga: A case study at Allahabad Stretch. Journal of Inland Fisheries Society of India. 46(1): 92-95.
  18. Khallaf, E.A., Galal, M., Authman, M. (2003). The biology of Oreochromis niloticus in a polluted canal. Ecotoxicology. 12: 405-416.
  19. Kim, Y-K., Lee, C-H., Lee, Y-D., Han, S-H. (2017) Development of species-specific PCR for the identification of three grouper fish species (Epinephelus septemfasciatus, E. bruneus and E. akaara). Indian Journal of Animal Research. DOI: 10.18805/ijar.v0iOF.8488.
  20. Lagler, K.F., Bardach, J.E., Miller, R.R. (1962). Ichthyology (The study of fishes). John Wiley, New York, 545 pp.
  21. Le Cren, E.D. (1951). The length-weight relationship and seasonal cycle in gonad weight and condition in the perch (Perca fluviatilis). Journal of Animal Ecology. 20: 201-219.
  22. Lee, D.J. and Kim, I-C. (2019). The new freshwater crab belongs to the genus Geothelphusa as inferred from mitochondrial and nuclear DNA markers. Indian Journal of Animal Research. DOI: 10.18805/ijar.B-1031. 
  23. Librado, P. and Rozas, J. (2009). DnaSP v5.0: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 25: 1451-1452. 
  24. Lowe, M.R, Wu, W., Peterson, M.S., Brown-Peterson, N.J., Slack, W.T., Schofield, P.J. (2012). Survival, growth and reproduc-
  25. -tion of non-native Nile Tilapia II: Fundamental Niche Projections and Invasion potential in the northern Gulf of Mexico. PLoS ONE. 7(7): e41580. 
  26. Mekkawy, I.A.A. and Mohammad, A.S. (2011). Morphometric and meristics of the three Epinepheline Species: Cephalopholis miniata (Forsskal,1775) and Variola louti (Forsskal,1775) from Red Sea, Egypt. Journal of Biological Sciences. 11: 10-21. 
  27. Mommsen, T.P. (1998). Growth and metabolism. In: The Physiology of the Fishes. D.H. Evans (Ed.). CRC Press, New York: 65-97.
  28. Nazrul, K.M.S., Mamun, A–A., Sarker, B.S., Tonny, U.S. (2011). Morphological variability of the 11th generation strain of nile tilapia, (Oreochromis niloticus) and traditional genetically improved farmed tilapia. Journal of Bangladesh Agricultural University. 9(2): 345–349.
  29. Olurin, K.B. and Aderibigbe, O.A. (2006). Length-weight relationship and condition factor of pond reared juvenile Oreochromis niloticus. World Journal of Zoology. 1: 82-85.
  30. Panda, D., Karna, S.K., Mukherjee, M., Manna, R. K., Suresh, V. R., Sharma, A. P. (2016). Length–weight relationships of six tropical fish species from Chilika Lagoon, India. Journal of Applied Ichthyology. 32: 1286–1289.
  31. Pauly, D. (1984). Fish population dynamics in tropical waters: A manual for use for programmable calculators. ICLARM studies and reviews 8: Manila, Philippines.
  32. Sambrook, J., Maccallum, P., Russel, D. (2001). Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Springs Harbour Press, NY, 2344 pp.
  33. Sarkar, U.K., Pathak, A.K., Sinha, R.K., Sivakumar, K., Pandian, A.K., Pandey, A., Dubey, V.K., Lakra, W.S. (2012). Freshwater fish biodiversity in the River Ganga (India): changing pattern, threats and conservation perspectives. Reviews in Fish Biology and Fisheries. 22: 251–72.
  34. Sangun, L and Guney, O.Y. (2017). Application of multiple regression analysis to morphometric characters obtained from Serranus cabrilla (linnaeus, 1758) by using stepwise method. Indian Journal of Animal Research. DOI: https://doi.org/10.18805/ijar.v0iOF.9185.
  35. Sen, S., Chakraborty, S.K., Elayaperumal, V., Zacharia, P,U., Jaiswar, A.K., et al. (2018). Reproductive strategy of milk shark, Rhizoprionodon acutus (Ruppell1837), along north eastern Arabian Sea Reproductive strategy of milk shark, Rhizoprionodon acutus (Ruppell1837), along north eastern Arabian Sea. Ichthyological Research. 65(3):324-333.
  36. Siddique, K.U., Islam, M.A., Kabir, S.M.H., Ahmed, M., Ahmed, A.T.A., et al. (2007). Encyclopedia of flora and fauna of Bangladesh. Volume 23. Freshwater fishes, Asiatic Society of Bangladesh, 300 pp.
  37. Snedecor, G.W. and Cochran, W.G. (1967). Statistical Methods. Oxford and IBH Publishing Co, New Delhi, 593 pp.
  38. Tamura, K., Peterson, D., Peterson, N., Stecher. G., Nei. M., Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance and Maximum Parsimony Methods. Molecular Biology and Evolution. 28: 2731-2739.
  39. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G. (1997). The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research. 25: 4876-4882.
  40. Trewavas, E. (1983). Tilapiine fishes of the genera Sarotherodon, Oreochromis and Danakilia. British Museum (Natural History), London, UK, 583 pp.
  41. Verma, H.O., Verma, S.K., Mishra, S.K., Alam, A., Das, S.C.S., Thakur, V., Kumar, J., et al. (2018). Length–weight relationships of Johnius coitor (Hamilton, 1822), Osteobrama cotio (Hamilton, 1822) and Gonialosa manmina (Hamilton, 1822), from the River Ganga, India. Journal of Applied Ichthyology. 34(6): 1361–1363.
  42. Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R., Hebert, P.D.N. (2005). DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society B: Biological Sciences. 360: 1847–1857.

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