Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 54 issue 6 (june 2020) : 716-722

Adaptation of wild strain of duck plague virus in cell culture systems

Nayanmoni Konwar, Hiramoni Sarmah, Sophia M. Gogoi, Kevisenuo Evalyn Vizo, Arpita Bharali, Nagendra N. Barman, Durlav P. Bora, Sailendra K. Das
1<div>Department of Microbiology, College of Veterinary Science,&nbsp;Assam Agricultural University, Khanapara, Guwahati-781 022, Assam, India.</div>
Cite article:- Konwar Nayanmoni, Sarmah Hiramoni, Gogoi M. Sophia, Vizo Evalyn Kevisenuo, Bharali Arpita, Barman N. Nagendra, Bora P. Durlav, Das K. Sailendra (2019). Adaptation of wild strain of duck plague virus in cell culture systems. Indian Journal of Animal Research. 54(6): 716-722. doi: 10.18805/ijar.B-3832.
Duck virus enteritis (DVE) is an acute, contagious herpes viral disease of ducks, geese and swans and members of the family Anatidae under the order Anseriformes. As per the recent taxonomic classification by ICTV, DEV has been classified into the genus Mardivirus, subfamily Alpha-herpesvirinae of the family Herpesviridae. Vaccination is the only option for the prevention and control of Duck plague. In India vaccination is done with chicken embryo adapted live virus which has many shortcomings. So, in this present situation a safe and potent vaccine development is the need of the hour. The use of chicken embryo fibroblast and a certified cell line may, probably, be the best option to achieve this. Therefore, the present study was undertaken for adaptation and propagation of local strain of duck plague virus in various cell culture systems.  During the study a wild strain of DPV (DP/As-Km/0019) which was isolated in Duck Embryo Fibroblast, available in the Department of Microbiology was selected. The selected wild strain was used for adaptation in various cell culture systems viz. Chicken embryo fibroblast cell culture (CEF), Vero cell line and QT-35 cell line. The virus was passaged up to 12th passage levels and the presence of viral antigen was demonstrated by appearance of cytopathic effect (CPE), by Polymerase Chain Reaction (PCR) and by Sandwich ELISA. PCR was able to detect virus from all the cell cultures from 5th passage onwards and virus titre was detected at 10th and 12th passage level by S-ELISA. It was observed that various cell culture systems can be a good candidate for propagation of DPV and further study is required to study its immunogenicity and its feasibility as a vaccine candidate.
  1. Ahamed,T; Hossain, K.M; Billah, M.M; Islam, K.M, Ahasan, M.M and Islam, M.E. (2004). Adaptation of Newcastle Disease Virus (NDV) on Vero Cell Line. International Journal of Poultry Science. 3(2): 153-156.
  2. Aravind S, Patil BR, Dey S, Mohan, C.M. (2012). Recombinant UL30 antigen-based single serum dilution ELISA for detection of duck viral enteritis. J Virol Methods., 185: 234-238.
  3. Aravind, S.; Kamble, N.M.; Gaikwad, S.S.; Shukla, S.K.; Dey, S. and Mohan, C.M. (2015). Adaptation and growth kinetics study of an Indian isolate of virulent duck enteritis virus in Vero cells. Microb. Pathog., 78: 14-9. 
  4. Baudet, A.E.R.F. (1923). Mortality in ducks in the Netherlands caused by a filterable virus Fowl plague. Tijdschr Diergeneeskd., 50: 455-459.
  5. Bhowmik, M.K. and Chakravarty, A.K. (1985). Occurrence of Duck plague in ducklings of West Bengal. Indian J. Poult. Sci., 20: 114-118. 
  6. Chakravarty, A.K.; Dutta, B.M.; Mukit, A.; Boro, B.R. and Bhattacharya, M.L. (1980). An outbreak of duck plague virus in Assam. J. Res. Assam Agric. Univ. 1: 72-78.
  7. Chandrika, P.; Kumanan, K.; Jayakumar, R. and Nachimuthu, K. (1999). Latex agglutination test for the detection of duck plague viral antigen. Indian Vet. J., 76: 372-374.
  8. Chellapandian, M; Piramamayagam, S. and Balachandram, S. (2005). Incidence of duck virus enteritis in Tirunelveli district of Tamil Nadu. Indian Vet., J. 82: 913.
  9. Dhama K., Kumar N., Saminathan M., Tiwari R., Karthik K., Kumar M.A., Palanivelu M., et al., (2017). Duck virus enteritis (duck plague)-a comprehensive update. Vet Q. 37(1): 57-80.
  10. Dinh, N.T.M.; Dung, N.V.; Hoang, H.V.; Hong, N.T.T.; Dung, D.V.; Trung, N.T.; Tu, T.D.; et al., (2004). Adaptation of duck plague virus to chicken embryo fibroblast cell culture for vaccine production. ACIAR Proceeding No. 117. In: Control of Newcastle Disease and Duck Plague in Village Poultry. [Meers, J.; Spradbrow, P.B. and Tu, T.D., editors.] Canberra, Australia, Australian Center for International Agricultural Research, 35-39. 
  11. Doley, M.; Das, S.K.; Barman, N.N. and Rajbongshi, G. (2013). Adaptation of vaccine strain of duck plague virus in in chicken embryo fibroblast cell culture. Indian J. Anim. Sci. 83(9): 880-882.
  12. Ferguson, N.M.; Fraser C; Riley S; Meeyai, A; Cummings, DA; Cauchemez, S. (2005). Strategies for containing an emerging influenza pandemic in South East Asia. Nature. 437: 209-214.
  13. GOI (2012). 19th Livestock Census 2012, All India Report, Ministry of Agriculture, Department of Animal Husbandry, Dairying and Fisheries, Government of India.
  14. Hansen, R.W.; Brown, E.S.; Nashold, W.S. and Knudson, L.D. (1999). Identification of duck plague virus by polymerase chain reaction. Avian Dis., 43: 106-115.
  15. Hung, D.; Phuc, K.V.; Trung, N.T.; Tu, T.D.; Huong, N.T.L. and Morrissy, C.J. (2004). Application of the antigen capture ELISA method for diagnosis of duck plague in Vietnam ACIAR Proceeding No.117. In: Control of Newcastle Disease and Duck Plague in Village Poultry. [Meers, J.; Spradbrow, P.B. and Tu, T.D. (eds.)], Canberra, Australia, Australian Center for International Agricultural Research, pp. 35-39. 
  16. Jaikumar,D; Read,K.M and Tannock, G.A. (2001) Adaptation of Marek’s disease virus to the Vero continuous cell line. Veterinary Microbiol., 79: 75-82
  17. Kamal,T. ; Muneer,A ; Ali ,Md.; Fatima, Z. and Ullah, A.(2015). Replication and adaptation of hydro pericardium virus (adenovirus-    4) on QT-35 cell line. Applied Sciences and Technology. 12th International Bhurban Conference.
  18. King, A.M.Q.; Adams, M.J.; Carstens, E.B. and Lefkowitz, E. J. (2012). Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, San Diego: 1344.
  19. Kulkarni, D.D.; James, P.C. and Sulochana, S. (1995). Isolation of duck plague virus from ducksin Kerala state. Ind. Vet. J., 72: 446-450.
  20. Kulkarni, D.D.; James, P. C. and Sulochana, S. (1998). Assessment of the immune response to duck plague vaccinations. Res. Vet. Sci., 64: 199-204.
  21. Kumar, Senthil ; Ponnoose, K .T. (1997) . Influence of Adaptation of the Vaccine Strain of Duck Plague Virus In: Chicken Embryo Fibroblast on Its Immunogenicity. Publisher: Mannuthy Dept.of Microbiology, College of Veterinary Science and Animal Husbandry.
  22. Leibovitz, L. (1991). Duck virus enteritis (Duck Plague). In: Disease of Poultry, 9th edition, [Calnek, B.W.; Barnes, H.J.; Beard, C.W.; Reid, W.M. and Yoder, H.W. (eds)], Iowa State University Press, Ames, 609-618.
  23. Mondal, B.; Rasool, T.J.; Ram, H. and Mallana, S. (2010). Propagation of vaccine strain of Duck virus enteritis in a cell line of duck origin as an alternative production system to Propagation in embryonated egg, Bilogicals. 38: 401-406.
  24. Mukherjee, A.; Das, M.S.; Ghosh, B.B. and Ganguly, J.L. (1963a). Duck plague in West Bengal. Part 1. Indian Vet. J., 40:457-62.
  25. Mukherjee, A.; Das, M.S.; Ghosh, B.B.; Ganguly, J.L. (1963b). Duck plague in West Bengal.Part II. Indian Vet. J., 40: 753-758. 
  26. Narhari, D. (2009). Housing and Management of ducks, IV World Waterfowl Conference, Thrissur, India, pp 45-47.
  27. Neher, S. (2016). Development of user friendly diagnostics and cell culture adapted vaccine candidate for duck plague (Ph.d Thesis), Assam Agricultural University.
  28. Neher, S.: Barman, N.N.; Bora, D.P.; Deka, D.; Tamuly, S.; and Deka, P.; Bharali, A. and Das, S.K. (2018). Detection and isolation of Duck Plague virus from field outbreaks in Assam, India. Indian J. Anim. Res. [online first article, DOI: 10.18805/ijar.B-3588].
  29. Nwajei, B.N.C; Afaleq, A. Al. and Jones, R.C. (1988) Comparison of chick embryo liver and vero cell cultures for the isolation and growth of avian reo viruses Avian Pathology. 17: 759-766.
  30. Pritchard, L.I.; Morrissy, C.; Van P.K.; Daniels, P.W. and Westerbury, H.A. (1999). Development of a polymerase chain reaction to detect Vietnamese isolates of duck virus enteritis. Vet microbiol., 68: 149-156.
  31. Rai, A. (2010). Method in Veterinary Virology, Allied Publisher. 
  32. Rajan, A.; Nair, M.K.; Maryama, K.I. and Valsala, K.V. (1980). Studies on epidemiology, symptoms and pathoanatomy of duck plague identification (Duck virus enteritis). Indian Vet. J., 57: 12-15.
  33. Rajput, D. S.; Singh, S. P.; Ghosh, S. and Nema, R.P. (2014). Duck Farming, fascinating option in India. J. Vet. Sci. Technol., 5: 3.
  34. Samah, S. Abou-Dalal 1, El-Bagoury, G.F 2, Suzan, K.Tolba1, Khodeir, M.H.(2013) Inactivation of Vero cell culture adapted Chicken Anaemia virus. BVMJ. 25(2)z: 55 62.
  35. Tu, T.D.; Phuc, K.V.; Huong, N.T.L.; Nind, L.; Hung, D.; Minh, T.D.; Thao, D.T.N. and Spradbrow, P. (2004). Isolation and identification of duck plague viruses from naturally infected ducks in southern Vietnam. Control of Newcastle Disease and Duck Plague in Village Poultry. Edited by Jonne Meere, Peter, B., Spradbrow and Tran Din Tu, ACIAR proceedings No.117. 
  36. Wang, G.; Qu, Y.; Wang, F.; Hu, D.; Liu, L.; Li, N.; Yue, R.; Li, C. and Liu, S. (2013). The comprehensive diagnosis and prevention of duck plague in northwest Shandong province of China. Poult. Sci., 92: 2892-2898.
  37. Xuefeng, Q.; Xiaoyan, Y.; Anchun, C.; Mingshu, W.; Dekang, Z. and Renyong, J. (2008a). The pathogenesis of duck virus enteritis in experimentally infected ducks: a quantitative time-course study using TaqMan polymerase chain reaction. Avi. Patho., 37 (3): 307-310.
  38. Yadav,S; Dah, B.B.; Kataria.J.M,; Dhama, K.; Gupta,S.K. and Rahul,S. (2007).Pathogenicity study of different avipoxviruses in embryonated chicken eggs and cell cultures. Indian Vet. J., 31: 17-20. 

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