Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 54 issue 5 (may 2020) : 601-607

Investigating immunomodulating activities of recombinant horse IL-2, IL-18 and IFN-ã in peripheral blood mononuclear cells (PBMCs)

Sheetal Saini, Harisankar Singha, Priyanka Siwach, Bhupendra Nath Tripathi
1ICAR-National Research Centre on Equines, Hisar-125 001, Haryana, India.
Cite article:- Saini Sheetal, Singha Harisankar, Siwach Priyanka, Tripathi Nath Bhupendra (2019). Investigating immunomodulating activities of recombinant horse IL-2, IL-18 and IFN-ã in peripheral blood mononuclear cells (PBMCs). Indian Journal of Animal Research. 54(5): 601-607. doi: 10.18805/ijar.B-3818.

ABSTRACT

Recombinant horse cytokines may be used as biological adjuvant for enhancing vaccine efficacy and therapeutic agent. This study describes low cost production of recombinant horse IL-2, IL-18 and IFN-ã in prokaryotic system. Immunomodulatory activities of recombinant horse IL-2, IL-18 and IFN-ã have been demonstrated in horse peripheral blood mononuclear cells (PBMCs). Recombinant horse IL-2 and IL-18 enhanced cellular proliferation and significantly up regulated transcription and secretion of IFN-ã, TNF-á, IL-4, IL-10 and IL-12p35. In contrast, recombinant horse IFN-ã was comparatively less active and did not show significant immune-stimulatory effect.

REFERENCES

  1. Bai, Y., Tong, T., Liu, G., Chen, W., Zhang, W., Wang, Q., Yang, T., Bu, Z. and Wu, D. (2010). Expression of biologically active recombinant equine interferon-gamma in Escherichia coli. Comparative Immunology, Microbiology and Infectious Diseases. 33: 333-342.
  2. Berghaus, L. J., Giguere, S., Bordin, A. I. and Cohen, N. D. (2018). Effects of priming with cytokines on intracellular survival and replication of Rhodococcus equi in equine macrophages. Cytokine, 102: 7-11.
  3. Budhia, S., Haring, L. F., McConnell, I. and Blacklaws, B. A. (2006). Quantitation of ovine cytokine mRNA by real-time RT-PCR. Journal of Immunological Methods. 309: 160-172.
  4. Cote-Sierra, J., Foucras, G., Guo, L., Chiodetti, L., Young, H. A., Hu-Li, J., Zhu, J. and Paul, W. E. (2004). Interleukin 2 plays a central role in Th2 differentiation. Proceedings of the National Academy of Sciences of the United States of America. 101: 3880-3885.
  5. Dohmann, K., Wagner, B., Horohov, D. W. and Leibold, W. (2000). Expression and characterisation of equine interleukin 2 and interleukin 4. Veterinary Immunology and Immunopathology. 77: 243-256.
  6. Figueiredo, M. D., Salter, C. E., Andrietti, A. L., Vandenplas, M. L., Hurley, D. J. and Moore, J. N. (2009) Validation of a reliable set of primer pairs for measuring gene expression by real-time quantitative RT-PCR in equine leukocytes. Veterinary Immunology and Immunopathology. 131: 65-72.
  7. Godornes, C., Leader, B. T., Molini, B. J., Centurion-Lara, A. and Lukehart, S. A. (2007). Quantitation of rabbit cytokine mRNA by real-time RT-PCR. Cytokine. 38: 1-7.
  8. Ju, G., Collins, L., Kaffka, K. L., Tsien, W. H., Chizzonite, R., Crowl, R., Bhatt, R. and Kilian, P. L. (1987). Structure-function analysis of human interleukin-2. Identification of amino acid residues required for biological activity. The Journal of Biological Chemistry. 262: 5723-5731.
  9. Kim, H. Y., Mott, J., Zhi, N., Tajima, T. and Rikihisa, Y. (2002). Cytokine gene expression by peripheral blood leukocytes in horses experimentally infected with Anaplasma phagocytophila. Clinical and Diagnostic Laboratory Immunology. 9: 1079-1084.
  10. Lin, Y. Z., Cao, X. Z., Li, L., Li, L., Jiang, C. G., Wang, X. F., Ma, J. and Zhou, J. H. (2011). The pathogenic and vaccine strains of equine infectious anemia virus differentially induce cytokine and chemokine expression and apoptosis in macrophages. Virus Research. 160: 274-282.
  11. Nakanishi, K., Yoshimoto, T., Tsutsui, H. and Okamura, H. (2001). Interleukin-18 regulates both Th1 and Th2 responses. Annual Review of Immunology. 19: 423-474.
  12. Okamura, H., Tsutsi, H., Komatsu, T., Yutsudo, M., Hakura, A., Tanimoto, T., et al. (1995). Cloning of a new cytokine that induces IFN-gamma production by T cells. Nature. 378: 88-91.
  13. Olejniczak, K. and Kasprzak, A. (2008). Biological properties of interleukin 2 and its role in pathogenesis of selected diseases—a review. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research. 14: RA179-189.
  14. Puddu, P., Carollo, M., Pietraforte, I., Spadaro, F., Tombesi, M., Ramoni, C., Belardelli, F. and Gessani, S. (2005). IL-2 induces expression and secretion of IFN-gamma in murine peritoneal macrophages. Journal of Leukocyte Biology. 78: 686-695.
  15. Razaghi, A., Owens, L. and Heimann, K. (2016). Review of the recombinant human interferon gamma as an immunotherapeutic: Impacts of production platforms and glycosylation. Journal of Biotechnology. 240: 48-60.
  16. Saikh, K. U. and Mott, T. M. (2017). Innate immune response to Burkholderia mallei. Current Opinion in Infectious Diseases. 30: 297-302.
  17. Saini, S., Singha, H., Siwach, P and Tripathi, B.N (2019). Recombinant horse IL-4 and IL-10 induced a mixed inflammatory cytokine response in horse peripheral blood mononuclear cells (PBMCs). Veterinary World. 12: 496-503.
  18. Sanchez-Matamoros, A., Kukielka, D., De las Heras, A. I. and Sanchez-Vizcaino, J. M. (2013) Development and evaluation of a SYBR Green real-time RT-PCR assay for evaluation of cytokine gene expression in horse. Cytokine. 61: 50-53.
  19. Sengupta, P., Meena, K., Mukherjee, R., Jain, S. K. and Maithal, K. (2008). Optimized conditions for high-level expression and purification of recombinant human interleukin-2 in E. coli. Indian Journal of Biochemistry and Biophysics. 45: 91-97.
  20. Sentsui, H., Wu, D., Murakami, K., Kondo, T. and Matsumura, T. (2010). Antiviral effect of recombinant equine interferon-gamma on several equine viruses. Veterinary Immunology and Immunopathology. 135: 93-99.
  21. Silva, E. B. and Dow, S. W. (2013). Development of Burkholderia mallei and pseudomallei vaccines. Frontiers in Cellular and Infection Microbiology. 3: 10.
  22. Singha, H., Goyal, S. k. and Malik, P. (2015). Cloning and sequence analysis of Interleukin (IL)-2, IL-4,IL-10, and IL-18 of Marwari horse. Indian Journal of Animal Sciences. 85: 621-627.
  23. Smith, K. A. (1984). Interleukin 2. Annual Review of Immunology. 2: 319-333.
  24. Steinbach, F., Mauel, S. and Beier, I. (2002). Recombinant equine interferons: expression cloning and biological activity. Veterinary Immunology and Immunopathology. 84: 83-95
  25. Stordeur, P., Poulin, L. F., Craciun, L., Zhou, L., Schandene, L., de Lavareille, A., Goriely, S. and Goldman, M. (2002). Cytokine mRNA quantification by real-time PCR. Journal of Immunological Methods. 259: 55-64.
  26. Strieter, R. M., Remick, D. G., Lynch, J. P., 3rd, Spengler, R. N. and Kunkel, S. L. (1989). Interleukin-2-induced tumor necrosis factor-    alpha (TNF-alpha) gene expression in human alveolar macrophages and blood monocytes. The American Review of Respiratory Disease. 139: 335-342.
  27. Tong, T., Bai, Y., Liu, G., Wang, Q., Zhang, W., Xiao, Y., Xu, S., Liu, N., Yang, T. and Wu, D. (2010). Expression, purification and monoclonal antibodies preparation of recombinant equine mature interleukin-18. Veterinary Immunology and Immunopathology. 136: 194-200.
  28. Vandergrifft, E. V. and Horohov, D. W. (1993). Molecular cloning and expression of equine interleukin 2. Veterinary Immunology and Immunopathology, 39:395-406.
  29. Wu, D., Murakami, K., Liu, N., Konishi, M., Muneta, Y., Inumaru, S., Kokuho, T. and Sentsui, H. (2004). Expression of equine interleukin-18 by baculovirus expression system and its biologic activity. Microbiology and Immunology. 48: 471-476.
  30. Xu, D., Trajkovic, V., Hunter, D., Leung, B. P., Schulz, K., Gracie, J. A., McInnes, I. B. and Liew, F. Y. (2000). IL-18 induces the differentiation of Th1 or Th2 cells depending upon cytokine milieu and genetic background. European Journal of Immunology. 30: 3147-3156.
  31. Yang, L., Wang, Y., MA, X., Wang, S and Zhang, L. (2016).Changes in expression of Th1 and Th2 cytokines in bovine peripheral blood mononuclear cells during early pregnancy. Indian Journal of Animal Research. 50: 466-470. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)