Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 54 issue 5 (may 2020) : 601-607

Investigating immunomodulating activities of recombinant horse IL-2, IL-18 and IFN-ã in peripheral blood mononuclear cells (PBMCs)

Sheetal Saini, Harisankar Singha, Priyanka Siwach, Bhupendra Nath Tripathi
1ICAR-National Research Centre on Equines, Hisar-125 001, Haryana, India.
Cite article:- Saini Sheetal, Singha Harisankar, Siwach Priyanka, Tripathi Nath Bhupendra (2019). Investigating immunomodulating activities of recombinant horse IL-2, IL-18 and IFN-ã in peripheral blood mononuclear cells (PBMCs). Indian Journal of Animal Research. 54(5): 601-607. doi: 10.18805/ijar.B-3818.
Recombinant horse cytokines may be used as biological adjuvant for enhancing vaccine efficacy and therapeutic agent. This study describes low cost production of recombinant horse IL-2, IL-18 and IFN-ã in prokaryotic system. Immunomodulatory activities of recombinant horse IL-2, IL-18 and IFN-ã have been demonstrated in horse peripheral blood mononuclear cells (PBMCs). Recombinant horse IL-2 and IL-18 enhanced cellular proliferation and significantly up regulated transcription and secretion of IFN-ã, TNF-á, IL-4, IL-10 and IL-12p35. In contrast, recombinant horse IFN-ã was comparatively less active and did not show significant immune-stimulatory effect.
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