Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 54 issue 5 (may 2020) : 563-569

Effect of Biofilm of Aeromonas Hydrophila Oral Vaccine on Growth Performance and Histopathological Changes in Various Tissues of Striped Catfish, Pangasianodon Hypophthalmus (Sauvage 1878)

M.A.A. Mamun, S. Nasren, P.B. Abhiman, S.S. Rathore, N.S. Sowndarya, K.S. Ramesh, K.M. Shankar
1Laboratory of Aquatic Animal Health Management, Depart of Aquaculture, College of Fisheries, Karnataka Veterinary Animal and Fisheries Science University, Mangalore-575 002, Karnataka, India.
Cite article:- Mamun M.A.A., Nasren S., Abhiman P.B., Rathore S.S., Sowndarya N.S., Ramesh K.S., Shankar K.M. (2019). Effect of Biofilm of Aeromonas Hydrophila Oral Vaccine on Growth Performance and Histopathological Changes in Various Tissues of Striped Catfish, Pangasianodon Hypophthalmus (Sauvage 1878). Indian Journal of Animal Research. 54(5): 563-569. doi: 10.18805/ijar.B-3814.
Biofilm of Aeromonas hydrophila oral vaccine was evaluated on growth and histopathological features of striped catfish, Pangasianodon hypophthalmus.  Fish were fed with feed incorporating biofilm (BF) or free cell (FC) of A. hydrophila vaccine at 1010 cells/fish/day for 20 days and basal feed for 60 days. From 30th day onwards, till end of the trial, the mean weight gain (g/fish) was significantly higher (P<0.05) in BF treated group than both FC and control group. At the end of the study, histopathological changes in gills, liver and kidney were evaluated using haematoxylin eosin technique. The histopathological findings, in BF fed P. hypophthalmus, showed normal architechture of the gills, liver and kidney. However, fishes fed on FC and control group, resulted several histopathological abnormalities in all the organs. 
  1. Abdullah-Al-Mamun, M., Al-Rashid, A. H., Mazumder, S. K., Shamsuzzaman, M. M., Hossain, M. M., Nasren, S. (2012). Improvement in the production phase of freshwater prawn, Macrobrachium rosenbergii in grow-out ponds through the use of substrates. J. Aquacult. Trop. 27(1-4): 53-64. 
  2. Azad, I. S., Shankar, K. M., Mohan, C. V., Kalita, B. (1999). Biofilm vaccine of Aeromonas hydrophila–standardization of dose and duration for oral vaccination of carps. Fish Shellfish Immunol. 9(7): 519-528.
  3. Azad, I. S., Shankar, K. M., Mohan, C. V., Kalita, B. (2000). Uptake and processing of biofilm and free-cell vaccines of Aeromonas hydrophila in Indian major carps and common carp following oral vaccination antigen localization by a monoclonal antibody. Dis. Aquat. Org. 43(2): 103-108.
  4. Azad, I. S., Shankar, K. M., Mohan, C. V. (1997). Evaluation of an Aeromonas hydrophila biofilm oral vaccination of carps. IM; Diseases in Asian aquaculture III (T.W. Flegal and I.H.Macrae, eds.), Fish health section, AFS, Manila, pp.181-186. 
  5. Babiñska, I., Rotkiewicz, T., Otrocka-Domaga³a, I. (2005). The effect of Lactobacillus acidophilus and Bifidobacterium spp. administration on the morphology of the gastrointestinal tract, liver and pancreas in piglets. Pol J Vet Sci. 8(1): 29-35.
  6. Balcázar, J. L., De Blas, I., Ruiz-Zarzuela, I., Cunningham, D., Vendrell, D., Muzquiz, J. L. (2006). The role of probiotics in aquaculture. Vet. Microbiol. 114(3-4): 173-186.
  7. Bernet, D., Schmidt, H., Meier, W., Burkhardt Holm, P., Wahli, T. (1999). Histopathology in fish: proposal for a protocol to assess aquatic pollution. J. Fish Dis., 22(1): 25-34.
  8. Bullock, A.M. (1989). Laboratory methods. In: Fish Pathology. [Edt. Roberts, R.J.] Bailliere Tindall, London, pp. 374-406.
  9. Dhar, A. K., Manna, S. K., Allnutt, F. T. (2014). Viral vaccines for farmed finfish. Virusdisease. 25(1): 1-17.
  10. Ellis, A.E. (1988). Optimizing factors for fish vaccination. In: Fish Vaccination (Ellis AE, editors): Academic Press, New York. pp. 32–46.
  11. Evensen, O. (2003). The vaccine formulation and its role in inflammatory processes in fish-effects and adverse effects. In: 3rd International Symposium on Fish Vaccinology, April 9–11, 2003, Bergen Norway.
  12. FAO. (2018). The State of World Fisheries and Aquaculture 2018- Meeting the sustainable development goals. Rome. Licence: CC BY-NC-SA 3.0 IGO.
  13. Ferguson, H. W., Turnbull, J. F., Shinn, A., Thompson, K., Dung, T. T., Crumlish, M. (2001). Bacillary necrosis in farmed Pangasius hypophthalmus (Sauvage) from the Mekong Delta, Vietnam. J. Fish Dis. 24(9): 509-513.
  14. Gao, Q., Xiao, C., Min, M., Zhang, C., Peng, S., Shi, Z. (2017). Effects of probiotics dietary supplementation on growth performance, innate immunity and digestive enzymes of silver pomfret, Pampus argenteus. Indian J Anim Res. 50(6): 936-941.
  15. Gobinath, J. and Ramanibai, R. (2014). Histopathological Studies in the gill, liver and kidney of the freshwater fish Labeo rohita fingerlings. Int. J. Innov. Res. Sci. Eng. Technol. 3(3): 10296.
  16. Hall-Stoodley, L., Costerton, J. W., Stoodley, P. (2004). Bacterial biofilms: from the natural environment to infectious diseases. Nat. Rev. Microbiol. 2(2): 95.
  17. Hoa, N. T., Baccigalupi, L., Huxham, A., Smertenko, A., Van, P. H., Ammendola, S., Cutting, S. M. (2000). Characterization of Bacillus species used for oral bacteriotherapy and bacterioprophylaxis of gastrointestinal disorders. Appl. Environ. Microbiol. 66(12): 5241-5247.
  18. Kumar, S., Raman, R. P., Prasad, K. P., Srivastava, P. P., Kumar, S., Rajendran, K. V. (2017). Modulation of innate immune responses and induction of oxidative stress biomarkers in Pangasianodon hypophthalmus following an experimental infection with dactylogyrid monogeneans. Fish Shellfish Immunol. 63: 334-343.
  19. Moneim, A. A., Elmenshawy, O., Al-Kahtani, M., Sayed, A., Alfwuaires, M. (2019). Pattern of renal pathology in fish from Al-Hassa waterways, Saudi Arabia. Indian J Anim Res. 53(6): 751-755.
  20. Nayak, D. K., Asha, A., Shankar, K. M., Mohan, C. V. (2004). Evaluation of biofilm of Aeromonas hydrophila for oral vaccination of Clarias batrachus-a carnivore model. Fish Shellfish Immunology. 16(5): 613-619.
  21. Nouh, W. G., Mohamed, M. F., Aly, S. M. (2009). Pathological evaluation to the effect of some probiotics on the health and immune status of Nile Tilapia (Oreochromis niloticus). Egyptain J Comp. Pathol. Clinical Pathol. 22(2). 233-249.
  22. Pournori, B., Paykan Heyrati, F., Dorafshan, S. (2017). Histopathological changes in various tissues of striped catfish, Pangasianodon hypophthalmus, fed on dietary nucleotides and exposed to water-borne silver nanoparticles or silver nitrate. J Aquat Anim Health. 3(2): 36-52.
  23. Ramesh, M. R., Shankar, K. M., Mohan, C. V., Varghese, T. J. (1999). Comparison of three plant substrates for enhancing carp growth through bacterial biofilm. Aquacult. Eng. 19(2): 119-131.
  24. Sharaf, M.M. and Tag, H.M. (2011). Growth performance, gill, liver and kidney histomorphology of common carp (Cyprinus carpio) fingerlings fed humic acid supplemented diets. Egypt J Exp Biol Zool. 7(2): 285-294.
  25. Sharma, S. K., Shankar, K. M., Sathyanarayana, M. L., Sahoo, A. K., Patil, R., Narayanaswamy, H. D., Rao, S. (2010). Evaluation of immune response and resistance to diseases in tiger shrimp, Penaeus monodon fed with biofilm of Vibrio alginolyticus. Fish Shellfish Immunol. 29(5): 724-732.
  26. Siriyappagouder, P., Shankar, K. M., Kumar, B. N., Patil, R., Byadgi, O. V. (2014). Evaluation of biofilm of Aeromonas hydrophila for oral vaccination of Channa striatus. Fish Shellfish Immunol. 41(2): 581-585.
  27. Sommerset, I., Krossøy, B., Biering, E., Frost, P. (2005). Vaccines for fish in aquaculture. Expert Rev Vaccines. 4(1): 89-101.
  28. Subagja, J., Slembrouck, J., Hung, L. T., Legendre, M. (1999). Larval rearing of an Asian catfish Pangasius hypophthalmus (Siluroidei, Pangasiidae): Analysis of precocious mortality and proposition of appropriate treatments. Aquat. Living Resour. 12(1): 37-44.
  29. Takashima, F. and Hibiya, T., (1995). An Atlas of Fish Histology: Normal and Pathological Features, 2nd Edition. Kodansha Ltd., Tokyo/ Fischer Verlag, Stuttgart/New York.
  30. Tatner, M.F. and Horne, M.T. (1986). Correlation of immune assays with protection in rainbow trout, Salmo gairdneri, immersed in Vibrio bacterins. J. Appl. Ichthyol. 2: 130–139.
  31. Thompson, F. L., Abreu, P. C., Wasielesky, W. (2002). Importance of biofilm for water quality and nourishment in intensive shrimp culture. Aquaculture. 203(3-4): 263-278.
  32. Toutain, C. M., Caiazza, N. C., O’Toole, G. A. (2004). Molecular basis of biofilm development by pseudomonads. In Microbial biofilms. American Society of Microbiology. pp. 43-63.
  33. Wali, A., Balkhi, M. U. H., Maqbool, R., Darzi, M. M., Bhat, F. A., Shah, F. A. (2018). Seasonal histopathological alterations caused by Adenoscolex oreini in Schizothorax niger of Kashmir valley. Indian J Anim Res. 52(6): 911-913.
  34. Zhang Q, Ma H M, Mai K S, Zhang W B, Liufu Z G, Xu W. (2010). Interaction of dietary Bacillus subtilis and fructooligosaccharide on the growth performance, non-specific immunity of sea cucumber, Apostichopus Japonicas. Fish Shellfish Immunol. 29: 204-211.

Editorial Board

View all (0)