Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 55 issue 4 (april 2021) : 457-462

Evaluation of Exogenous Laccase Enzyme Treated Finger Millet Straw on Body Weight Gain, DM Intake and Nutrient Digestibility in Heifers

Vidya Pradeep Kumar, Ramya G. Rao, A. Dhali, Vandana Thammiaha, Manpal Sridhar
1ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru-560 030, Karnataka, India.
Cite article:- Kumar Pradeep Vidya, Rao G. Ramya, Dhali A., Thammiaha Vandana, Sridhar Manpal (2021). Evaluation of Exogenous Laccase Enzyme Treated Finger Millet Straw on Body Weight Gain, DM Intake and Nutrient Digestibility in Heifers. Indian Journal of Animal Research. 55(4): 457-462. doi: 10.18805/IJAR.B-3807.
Background: Fungal laccases have widespread application in a number of biotechnological processes including the biodegradation of lignin. Their low yield in the native state limits their practical use in the deconstruction of lignocellulosic biomass for feeding ruminants. Enzymes in bulk quantities are required to treat biomass which has got greater product consistency and less lot to lot variations. The present study was an attempt to evaluate the effect of feeding finger millet straw treated with exogenous laccases in heifers.
Methods: The effect of feeding exogenous laccases obtained from immobilized Schizophyllum commune (MTCC 11893) on body weight gain, dry matter intake and nutrient digestibility in heifers was evaluated in three groups of heifers (4-each) with body weights ranging between 275 ± 47.12 to 276.75 ± 64.48kg. The control group received ad lib. finger millet straw treated with only production media (GI). Test group 1 (GII) received ad lib. straw treated with laccase rich media in a 3:5 (w/v) ratio, while test group 2 (GIII) received ad lib. straw treated in a 4:5 (w/v) ratio. 
Result: After 14- days of feeding, gain in body weights for GI, GII and GIII were 277.9 ± 68.47; 277.50 ± 46.43 and 278.85 ± 37.22 respectively with an overall increase of 1.15, 4.25 and 3.60 kgs. No significant variation (P>0.05) was observed with regard to DMD (%) between the groups which was 46 ± 5.8 for the control animals and 41±13.0 and 41± 3.4 in G2 and G3 groups. Though significant variations were observed digestibility studies proved inconclusive. Though preliminary results indicate that applying lignin degrading enzymes as feed supplements could enhance digestibility of crop residues in ruminants.
  1. AOAC. (2016). Official Methods of Analysis, 20th edition, Association of Official Analytical Chemist, edited by Airlington VA, 2016.
  2. Arora, D.S., Chander, M. and Gill, P.K. (2002). Involvement of lignin peroxidase, manganese peroxidase and laccase in degradation and selective ligninolysis of wheat straw. International Journal of Biodeterioration and Biodegradation. 50: 115-20.
  3. Beauchemin, K.A., Colombatto, D., Morgavi, D.P. and Yang, W.Z. (2003). Use of exogenous fibrolytic enzymes to improve feed utilization by ruminants. Journal of Animal Science. 81(E.Suppll. 2): E37-E47.
  4. Bowman, G.R., Beauchemin, K.A. and Shelford, J.A. (2002). The proportion of the diet to which fibrolytic enzymes are added affects nutrient digestion by lactating dairy cows. Journal of Dairy Science. 85(12): 3420-3429.
  5. Bourbonnais, R., Leech, D. and Paice, M.G. (1998).Electrochemical analysis of the interactions of laccase mediators with lignin model compounds. Biochemistry Biophysics Acta. 1379 (3): 381-390.
  6. Galhaup, C., Wagner, H., Hinterstoisser, B. and Haltrich, D. (2002). Increased production of laccase by the wood- degrading basidiomycetes Trametes pubescens. Enzyme Microbial Technology. 30: 529-536.
  7. Gencoglu, H., Shaver, R.D., Steinberg, W., Ensink, J., Ferraretto, L.F., Bertics, S.J. et al. (2010). Effect of feeding a reduced- starch diet with or without amylase addition on lactation performance in dairy cows. Journal of Dairy Science. 93(2): 723-732.
  8. Hristov, A.N., McAllister, T.A. and Cheng, K.J. (2000). Intraruminal supplementation with increasing levels of exogenous polysaccharidase-degrading enzymes: Effects on nutrient digestion in cattle fed a barley grain diet. Journal of Animal Science. 78: 477-87.
  9. Tilley, J.M.A. and Terry, R.A. (1963). A two-stage technique for the in vitro digestion of forage crops. Journal of British Grassland Society. 18(2): 104-111.
  10. Krishna Prasad, V., Venkata Mohan, S., Srinivas, R., Pati, B.R. and Sarma, P.N. (2005). Optimization of actual condition for laccase production by Taguchi orthogonal array experimental design, Biochemical Engineering Journal. 24: 17-26.
  11. Kumar, V.P., Naik, C., Sridhar, M. (2015). Production, purification and characterization of novel laccase produced by Schizophyllum commune NI07 with Potential for delignification of crop residues. Applied Biochemistry and Microbiology. 51(4): 432-441.
  12. Lowry, O.H., Rosebrough, N.J., Farr, L. and Randall, R.J. (1951). Protein measurement with the Folin Phenol Reagent. Journal of Biological Chemistry.193: 267-75.
  13. Martins, A.S., Vieira, P.D.F., Berchielli, T.T., Prado, I.N. and Moletta, J.L. (2006). Intake and apparent total tract digestibility in cattle supplemented with fibrolytic enzymes. Revista Brasileira de Zootecnia. 35(5): 2118-2124.
  14. Nsereko, V.L., Beauchemin, K.A., Morgavi, D.P., Rode, L.M., Furtado, A.F., McAllister, T.A, Iwaasa, A.D., Wang, W.Z. and Wang, Y. (2002). Effect of a fibrolytic enzyme preparation from Trichodermalongi brachiatum on the rumen microbial population of dairy cows. Canadian Journal of Microbiology. 48: 14-20.
  15. Oliveira, L.G. de., Ferreira, R.N., Padua, J.T.,,Ulho, C.J., Cysneiros, C.D.S.S., Arnhold, E. (2015). Performance of beef cattle bulls in feed lots and fed on diets containing enzymatic complex. Acta Science. Animal Science. [online]. 37(2): 181-186. 
  16. Rodrigues, M.A.M., Pinto, P., Bezerra, R.M.F., Dias, A.A., Guedes, C.V.M., Gardoso, V.M.G., Cone, J.W., Ferreira, L.M.M., Colaco, J. and Sequeira, C.A. (2008). Effect of enzyme extracts isolated from white-rot fungi on chemical composition and in vitro digestibility of wheat straw. Animal Feed Science and Technology. 141: 326-338.
  17. Saritha, M., Arora, A. and Lata (2012). Biological pretreatment of lignocellulosic substrates for enhanced delignification and enzymatic digestibility. Indian Journal of Microbiology. 52 (2):122-130.
  18. SAS (2009). SAS Institute Inc. Cary. North Carolina, U.S.A. 
  19. Sridhar, M., Bhatta, R., Dhali, A., Saravanan, M., Vidya, P., Vandana, T. (2015). Effect of exogenous lignolytic enzyme-treated finger millet straw on DM intake, digestibility, rumen fermentation and rumen enzymes in sheep. Indian Journal of Animal Sciences. 85(9): 1012-1016.
  20. Steel, R.G.D. and Torrie, J.H. (1980). Principles and Procedures of Statistics, Second Edition, New York: McGraw-Hill.
  21. Van Soest, P.J., Robertson, J.B. and Lewis, B.A. (1991). Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science.74: 3583-97.

Editorial Board

View all (0)