Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 54 issue 4 (april 2020) : 399-404

Polymorphism in inhibin alpha gene and its association with semen quality traits in Murrah bulls

Satish Chandra, D.N. Das, Uday Kannegundla, Jagish Kour Reen, K.P. Ramesha, Sapna Nath, Mukund A. Kataktalware
1Southern Regional Station ICAR-Indian Dairy Research Institute, Bengaluru-560 030, Karnataka, India.
Cite article:- Chandra Satish, Das D.N., Kannegundla Uday, Reen Kour Jagish, Ramesha K.P., Nath Sapna, Kataktalware A. Mukund (2019). Polymorphism in inhibin alpha gene and its association with semen quality traits in Murrah bulls. Indian Journal of Animal Research. 54(4): 399-404. doi: 10.18805/ijar.B-3798.
An investigation was conducted to detect single nucleotide polymorphism in Inhibin alpha gene (INHA) and to analyse the association between the observed polymorphisms with semen quality traits in Murrah buffalo bulls. Genomic DNA was isolated from blood samples of 103 bulls, the amplified PCR products were further subjected to polyacrylamide gel electrophoresis. Band patterns were characterised by the number of bands, mobility shifts and scored manually. Representative PCR products giving unique SSCP patterns were custom sequenced using automated ABI DNA Sequencer to confirm the mobility shift in each pattern. Sequence data were analysed using DNA Baser and Bio edit software Clustal omega multiple alignments for detecting single nucleotide polymorphisms in comparison to observed sequence with the Bubalus sbubalis INHA gene reference sequence. INHA gene, exon1 was monomorphic, while exon 2 was found to be polymorphic. For simple interpretation of SSCP patterns, INHA exon 2 was divided into two fragments. The first fragment of INHA exon 2 (INHA exon 2 FR1) revealed a transition at the C267119T position and a transversion at the G267033T position in the coding region in comparison to Bubalus bubalis reference sequence. The second fragment of INHA exon 2 (INHA exon 2 FR2) exhibited one transition at T266510C position in comparison to the Bubalus bubalis reference sequence. The SSCP variants of the first fragment of INHA exon2 had a significant effect on semen volume per ejaculate (P<0.05) and sperm concentration (P<0.01). The SSCP variants of the second fragment of INHA exon 2 had a significant effect on sperm concentration (P<0.01) and functional membrane integrity (P<0.05). The observed association between SSCP variants in INHA gene with semen volume per ejaculate, sperm concentration and functional membrane integrity suggests the possibility of using these genetic variants in INHA gene as prognostic markers for selecting Murrah bulls with high semen quality traits.
  1. Barani, A., Ramesha, K. P., Basavaraju, M., Akhila, R. and Rao, T. V. L. N. (2017). Genetic characterization and identification of single nucleotide polymorphisms of ATP1A1 gene in Indian humped cattle. Indian Journal of Animal Research, 51(1): 58-63.
  2. Fortes, M.R.S., Reverter, A., Kelly, M., McCulloch, R. and Lehnert, S.A. (2013). Genome wide association study for inhibin, luteinizing hormone, insulin like growth factor 1, testicular size and semen traits in bovine species. Andrology, 1: 644-650.
  3. Giesecke, K., Hamann, H., Sieme, H., Distl, O. (2010). INHBA-associated markers as candidates for stallion fertility. Reprod. Domest. Anim., 45: 342–347.
  4. Lin, C.L., Ponsuksili, S., Tholen, E., Jennen, D.G.J., Schellander, K., Wimmers, K. (2006). Candidate gene markers for sperm quality and fertility of boar. Anim. Reprod. Sci., 92: 349–363.
  5. Mason, A.J., Hayflick, J.S., Ling, F., Esch, N., Ueno, S.Y., Ying, R., Guillemin, H.N., Seeburg, P.H. (1985). Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor-â. Nat., 318: 659-663. 
  6. Mathevon, M., Buhr, M.M., Dekkers, J.C.M. (1998). Environmental, management, and genetic factors affecting semen production in Holstein bulls. J. Dairy Sci., 81: 3321-3330.
  7. Miller, S.A., Dykes, D.D., Polesky, H.F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res., 16: 1215.
  8. Rivier, J., Spiess, J., McClintock, R., Vaughan, J., Vale, W. (1985). Purification and partial characterization of inhibin from porcine follicular fluid. Biochem.Biophys. Res. Commun., 133: 120-127.
  9. Sambrook, J. and Russell, D.W. (2001). Molecular cloning A laboratory manual 1st volume, New York: Cold Spring Harbor Laboratory Press, 1.32-1.34 and 8.14p.
  10. Sang, L., Du, Q.Z., Yang, W.C., Tang, K.Q., Yu, J.N., Hua, G.H., Zhang, X.X., Yang, L.G. (2011). Polymorphisms in follicle stimulation hormone receptor, inhibin alpha, inhibin bata A, and prolactin genes, and their association with sperm quality in Chinese Holstein bulls. Anim. Reprod. Sci., 126: 151-156. 
  11. Seok, O.S., Ahn, J.M., Mayo, K.E., Cho, B.N. (2004). Developmental changes in inhibin-alpha gene expression in the mouse testis. Mol. Cells., 17: 67–72.
  12. Toulis, K.A., Iliadou, P.K., Venetis, C.A., Tsametis, C., Tarlatzis, B.C., Papadimas, I., Goulis, D.G. (2010). Inhibin B and anti-    Müllerian hormone as markers of persistent spermatogenesis in men with non-obstructive azoospermia: a meta-analysis of diagnostic accuracy studies. Hum. Reprod. Update., 16: 713-724.

Editorial Board

View all (0)