Indian Journal Of Animal Research

Genetic polymorphism in TLR2 genes of HF cross bred cattle through PCR SSCP

DOI: 10.18805/ijar.B-3796    | Article Id: B-3796 | Page : 430-433
Citation :- Genetic polymorphism in TLR2 genes of HF cross bred cattle through PCR SSCP.Indian Journal Of Animal Research.2020.(54):430-433
P.V. Jadhav, D.N. Das and S.B. Tarate drprajaktavet@gmail.com
Address : Department of Animal Genetics, South Regional Station of National Dairy Research Institute,Adugodi, Bangalore-560 030, Karnataka, India.
Submitted Date : 7-02-2019
Accepted Date : 30-04-2019
First Online:

Abstract

TLRs are cell-surface receptors which recognize a broad class of pathogen-associated molecular patterns (PAMPs) that activates innate and adaptive immune responses. Thorough perusal of literature suggests a possible role of TLR2 gene in resistance to infection due to gram positive bacteria. Bovine TLR2 assigns to Bos taurus (BTA) chromosome 17 it spans over 13.2 kb of genomic DNA which includes 2 exons. The first exon is spread over 179bp while the second one is 3333bp. Molecular characterization of TLR2 gene, carried out by PCR- SSCP analysis, revealed presence of six SNPs viz., A827G (exon 2.2), C1088G (exon 2.3), C2155A (exon 2.5), G2281C (exon 2.5), G2410A (exon 2.6) and C2600T (exon 2.6). Gene and genotypic frequencies were determined by values for allele frequencies and hetrozygosity. The degree of heterozygosities, c2 (Chi-square) test for Hardy Weinberg (HW) equilibrium and F statistics were performed for the HF crossbred population. Population genetic analysis demonstrated high degree of genetic differentiation in the population. The studied population displayed a deviation from the HW equilibrium. 

Keywords

Allelic frequencies HF cattle HW equilibrium Shannon index TLR2.

References

  1. Akira, S., Takeda, K., Kaisho, T. (2001). Toll-like receptors: critical proteins linking innate and acquired immunity. Nature Immunology. 2: 675–680.
  2. Bai, J., Jia-peng, L., Fang, Y., Min, H., Wen-rong, L. and Min-Jun, L. (2011). Association of Toll like Receptor 2 polymorphism with somatic cell score in bovine. Acta Veterinaria et Zootechnica Sinica. 42 (3): 350-362.
  3. Iwasaki, A. and Medzhitov, R. (2004). Toll-like receptor control of the adaptive immune responses. Nature Immunology. 5:987 – 995.
  4. Kimura, M. and Crow, J.F. (1964). The number of alleles that can be maintained in a finite population. Genetics. 49: 725- 738.
  5. Lewontin, R.C. (1972). The apportionment of human diversity. Evolutionary Biology. 6: 381-398.
  6. Mariotti, M., Williams, J., Dunner, S., Valentini, A. and Pariset, L. (2009). Polymorphism within the Toll-Like Receptor (TLR)-2, -4, and -6 Genes in Cattle. Diversity. 1: 7-18.
  7. McGuire, K., Jones, M., Werling, D., Williams, J.L., Glass, E.J. and Jann, O. (2006). Radiation hybrid mapping of all 10 characterized bovine Toll-like receptors. Animal Genetics. 37 (1): 47–50.
  8. Miller, S.A., Dykes, D.D. and Polesky, H.F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acid Research. 16: 1215
  9. Shizuo, A., Kiyoshi, T. and Tsuneyasu, K.( 2001).Toll-like receptors: critical proteins linking innate and acquired immunity. Nature Immunology. 2: 675-680
  10. Yeh, F., Yang, R. and Boyle, T. (2006). POPGENE version 1.31. Microsoft Windows-based freeware for population genetic analysis. University of Alberta and Centre for International Forestry Research, Canada. Available at (http: // www.ualberta.ca /~fyeh / fyeh).
  11. Zhang, C.X., Wang, H.M., Li, J.B., Wang, C.F., Lai, S.J., Li,Q.L. and Zhong, J.F. (2009). Polymorphism of TLR-2 gene and the relationship between the gene and mastitis resistance in Chinese Holstein. Chinese Journal of Veterinary Science. 29 (8): 1065-1068.

Global Footprints