Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 3 (march 2020) : 282-285

Novel SNP identified in HSBP1 gene and its association with thermal tolerance traits in Murrah buffalo

J. Saikia, A. Verma, I.D. Gupta, D. Hazarika, B. Deshmukh, R. Das
1Dairy Cattle Breeding Division, ICAR-National Dairy Research Institute, Karnal-132 001, Haryana, India.
Cite article:- Saikia J., Verma A., Gupta I.D., Hazarika D., Deshmukh B., Das R. (2019). Novel SNP identified in HSBP1 gene and its association with thermal tolerance traits in Murrah buffalo. Indian Journal of Animal Research. 54(3): 282-285. doi: 10.18805/ijar.B-3767.

ABSTRACT

Heat shock response (HSR) is a universal mechanism in all organisms upon heat shock (HS) to prevent stress damages. It is under tight regulation by heat shock factors (HSFs) and heat shock proteins (HSPs). On the attenuation of HSR, Heat Shock Factor Binding Protein1 (HSBP1) interacts with HSF1 and thus converting the active trimer form of HSF1 to inert monomer state. Thus, play an important role in regulating heat shock genes. The present study aimed to explore single nucleotide polymorphism (SNP) in HSBP1 and their association with the thermo tolerance traits (respiration rate, rectal temperature and heat tolerance coefficient) for identification of most thermal adapted Murrah buffaloes. Two hundred female Murrah buffaloes were genotyped using custom sequencing to identify SNP. Only one novel SNPs in exon 3 at position g.1892C>T and two nucleotide variation g.1327C>G and g.1202C>T were identified through direct DNA sequence scanning. Homozygous wild type genotypes (CC) were found in higher frequency (0.84, n=168) than homozygous recessive (0.10, n=20) and heterozygous (0.06, n=12) mutant genotypes at SNP locus g.1892C>T. The association among the different genotype of this SNP locus with thermo tolerance was analyzed using Generalized Linear Model procedure in Statistical Analysis System. However, no significant difference was detected among the genotypes in terms of thermo tolerance traits in the study.

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