Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 51 issue 1 (february 2017) : 135-140

Studying the protective effect of gamma-irradiated basil (Ocimum basilicum L.) against methotrexate-induced liver and renal toxicity in rats

A.N. El Shahat1*, H.M. El-Shennawy1, Mohamed H.M. Abd el-Megid2
1<p>Food Irradiation Research Department, National Centre for Radiation Research and Technology,&nbsp;<br /> Atomic Energy Authority, Cairo, Egypt.</p>
Cite article:- Shahat1* El A.N., El-Shennawy1 H.M., el-Megid2 Abd H.M. Mohamed (2016). Studying the protective effect of gamma-irradiated basil (Ocimum basilicum L.) against methotrexate-induced liver and renal toxicity in rats . Indian Journal of Animal Research. 51(1): 135-140. doi: 10.18805/ijar.9631.

The efficacy of methotrexate (MTX), a widely used cytotoxic chemotherapeutic agent, is often limited by severe hepatic and renal toxicity. Basil has shown antioxidant and antimicrobial activities due to its phenolic and aromatic compounds. Here, the aim of this work was to investigate the effect of g-radiation processing on the total phenolic and total flavonoids content of dried basil as well as to study the protective role of g-irradiated dried basil leaves against MTX-induced hepatic and renal toxicity in rats. Results obtained that g-radiation treatment increased the amount of total phenolic and total flavonoids content of dried basil. Additionally, the results demonstrated that the MTX (in saline, 20 mg/kg) given to rats on the day 21 of the experiment resulted in significant elevation in the activities of serum alanine and aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase  and the levels of serum urea, creatinine and uric acid as well as the concentration of hepatic and renal malondialdehyde and xanthine oxidase accompanied with a depression in hepatic glutathione content and xanthine dehydrogenase, superoxide dismutase and catalase activities in comparison with control and treated groups. Addition of either raw or g-irradiated basil (1%) to the diet of MTX-rats has significantly reduced the activities of serum enzymes and the levels of serum urea, creatinine and uric acid accompanied by ameliorating the alteration in the antioxidant/oxidant status of the liver and kidney. It was concluded that basil can protect against MTX-induced hepatic and renal injury and may be of therapeutic potential in alleviating the systemic side effects of this chemotherapeutic agent.


  1. Aebi, H. (1984). Catalase in vitro. Methods Enzymol., 105: 121–126.

  2. Ahmed, H.A., Sadek ,K.M. and Taha A. E. (2015b). Impact of Two Herbal Seeds Supplementation on Growth Performance and Some Biochemical Blood and Tissue Parameters of Broiler Chickens International. Journal of Biological, Food, Veterinary and Agricultural Engineering 9: 2015.

  3. Ahmed, W, Zaki A. and Nabil T. (2015a). Prevention of methotrexate-induced nephrotoxicity by concomitant administration of garlic aqueous extract in rat. Turkish Journal of Medical Sciences 45:1-10.

  4. Akaby T., Sehirli, O., Ercan , F., and Sener, G., (2010). Resveratrol Protects Against Methotrexate-Induced Hepatic Injury in Rats. Tugba J Pharm Pharmaceut Sci 13: 303 – 310.

  5. AL-Motabagani , M.A. (2006). Histological and histochemical studies on the effects of mehotrexate on the liver of adult male albino rat. Int. J. Morphol. 24: 417-422.

  6. Božin, B., Mimica-Dukiæ, N., Samojlik, I., Anaèkov, G., Igiæ, R. (2008). Phenolic in garlic (Allium sativum L., Alliacea). Food Chem. 111:925-929.

  7. ÇakIr ,T., BaStürk, A., Polat, C., Aslaneriv, A., Durgutv, H., Sehirliv, A. Ö., Gülv, M., Ögünçv A. V., Gülix ,S., , Sabuncuoglux, M.Z. and Oruçx, M. T. (2015). Does alfa lipoic acid prevent liver from methotrexate induced oxidative injury in rats? Acta Cirúrgica Brasileira . 30: 247-254

  8. Coleshowers, C.L, Oguntibeju, O.O, Ukpong, M. and Truter, E.J. (2010). Effects of methotrexate on antioxidant enzyme status in a rodent model. Medical Technology SA. 24:1-9.

  9. Coulomb, J.J. and Farreau, L. (1963). A new simple semi-micro method for colourimetric determination of urea. Clinical Chemistry. 9: 102.

  10. Dasgupta T., Rao A. and Yadava P. (2007). Chemomodulatory efficacy of basil leaf (Ocimum Basilicum) on drug metabolizing and antioxidant enzymes, and on carcinogen induced skin and forestomach papillomagenesis. Phytomedicine.11:139-151.

  11. Fukumoto, L. and Mazza, G. (2000). Asessing antioxidant and prooxidant activities of phenolic compounds. J. Agric. Food Chem. 48: 3597-3604.

  12. Gross, R.T., Bracci, R., Rudolph, N., Schroeder, E. and Kochen, J.A. (1967). Hydrogen peroxide toxicity and detoxification in the erythrocytes of new born infants. Blood. 29: 481-493.

  13. Husdan, H. and Rapoport, A. (1968). Estimation of the creatinine by the Jaffe reaction. A comparison of three methods. Clinical Chemistry. 14: 222.

  14. Jia, Z., Tang, M. and Wu, J. (1999). The determination of flavonoids content in mullbery and their scavenging effects on superoxide radicals. Food Chem. 64:555-559.

  15. Kaminski, Z.W. and Jewezska, M.M. (1979). Intermediate dehydrogenase oxidase form of xanthine oxidoreductase in rat liver. Biochem J. 181: 177–182.

  16. Kandasamy, C.S., Shimna, T.P., Mohammed, B.E., Arul, R.P., Gopal, V. and Venkatnarayanan, R. (2010). Anti-hepatotoxic activity of polyherbal formulation in carbon tetrachloride induced toxicity in rats. RJPBCS. 1:342-346.

  17. Karamala, S.K., Srilatha, C., Anjaneyulu, Y., ChandraSekharaRao, T.S., Sreenivasulu, D. and Pidugu, A.P. (2011). Haemato-    biochemical changes of lead poisoning and amelioration with Ocimum sanctum in Wistar albino rats. Vet. World. 4: 260-263.

  18. Khoun O.S.(2012). Pharmacological effect of aqueous leaves extract of Ocimum basilicum against liver toxicity induced by Acetaminophen in male rats. Thi-Qar J. Agric. Res. 1: 13-27. 

  19. Kind, P. and King, E. (1954). Estimation of plasma phosphatase by determination of hydrolysed phenol with aminoantipyrine. J. Clin. Pathol. 7: 322.

  20. Kolli, V.K., Abraham, P. and Rabi, S. (2008). Methotrexate-induced nitrosative stress may play acritical role in small intestinal damage in the rat. Archives of Toxicology. 82: 763-770.

  21. Kose, E., Sapmaz, H.I., Sarihan, E., Vardi, N., Turkoz, Y. and Ekinci, N. (2012). Beneficial effects of Montelukast against Methotrexate induced liver toxicity: A biochemical and histological study, The Scientific World Journal., 2012:987508.

  22. Kumari, N., Kumar, P., Mitra, D. , Prasad, B., Tiwary, B. N. and Varshney, L. (2009). Effects of ionizing radiation on microbial decontamination, phenolic contents, and anti-oxidant properties of Triphala. J. Food Sci. 74:M109-M1113. 

  23. Kuriakose, G.C. and Kurup, M. G. (2010). Hepatoprotective effect of Spirulina lonar on paracetamol induced liver damage in rats. Asian J. Biol. Sci.1:.614-623

  24. Makwana, M. and Rathore, H.S. (2011).Prevention of hepatorenal toxicity of acetaminophen with Ocimum sanctum in mice. I. J. P. T. 3: 1385-1396.

  25. Mastaneh, M., Ahmad, M., Taher, N. and Mehrdad, H. (2014). Antioxidant effect of Ocimum basilicum cv. dark opal (Lamiaceae) Phenolics .Orient. J. Chem., 30: 1965-1969 .

  26. Minami, M. and Yoshikawa, H. (1979). A simplified assay method of superoxide dismutase activity for clinical use. Clin. Chim. Acta. 92: 337-342.

  27. Oboh, F.O.J., Madsodje, H.I. and Enabulele, S.A. (2009). A nutritional and antimicrobial property of Ocimum gratissimum leaves. J. Biol. Sci.. 9:377-380.

  28. Parveen, S., Hussain, P. R., Mir, M. A., Shafi F., Darakshan, S., Mushtaqand, A.and Suradkar P. (2015). Gamma irradiation treatment of cherry : impact on storage quality, decay percentage and post- refrigeration shelf-life extension. Current Research in Nutrition and Food Science. 3: 54-73.

  29. Reitman, S. and Frankel, S. (1957). A calorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28: 56.

  30. Rosalki, S.B. (1975). Gamma-glutamyltranspeptidase. Adv. Clin. Chem. 17:53–107.

  31. Sakr, S.A., El-Abd, S.F., Osman, M., Kandil, A.M. and Helmy, M.S. (2011). Ameliorative effect of aqueous leave extract of Ocimum basilicum on CCl4 - induced hepatotoxicity and apoptosis in Albino rats. J. Am. Sci. 7:116-27.

  32. Sakr, S.A. and Al-Amoudi W. M. (2012). Effect of leave extract of Ocimum basilicum on deltamethrin induced nephrotoxicity and oxidative stress in albino rats. JAPS. 02: 22-27.

  33. SPSS. (1998). Statistical Package for Social Science. Computer Software, Ver. 10. SPSS Company, London, UK.

  34. Swayeh, N. H., Abu-Raghif, A. R., Qasim, B. J. and Sahib H. B. (2014). The protective effects of Thymus vulgaris aqueous extract against Methotrexate- induced hepatic toxicity in rabbits. Int. J. Pharm. Sci. Rev. Res. 29: 187-193.

  35. Tousson, E., Zaki, Z.T., Abu-Shaeir, W.A. and Hassan, H. (2014). Methotrexate-induced hepatic and renal toxicity: Role of L-carnitine in treatment. Biomedicine and Biotechnology. 2: 85-92

  36. Villavicencio, A.L.C.H., Mancini-Filho, J., Delincée, H. and Greiner, R. (2000). Effect of irradiation on anti-nutrients (total phenolics, tannins and phytate) in Brazilian beans. Rad. Phy. and Chem.. 57: 289–293.

  37. Yacout, G. A., Elguindy, N. M. and El Azab E.F. (2012). Hepatoprotective effect of basil (Ocimum basilicum L.) on CCl4-    induced liver fibrosis in rats. African J.Biotech. 11: 15702-15711.

  38. Yoshioka, T., Kawada, K., Shimada, T. and Mori, M. (1979). Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am. J. Obstet. Gynecol. 135: 372-376.

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