Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 54 issue 5 (may 2020) : 523-528

PCR- RFLP Polymorphism at Exon 3 and Promoter Region of Prolactin Gene in Gaolao Cattle

P.D. Dudule, D.S. Kale, A. Sonwane, D.V. Patil, M.R. Jawale, K.P. Kharkar
1Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Seminary Hills, Nagpur-440 006, Maharashtra, India.
Cite article:- Dudule P.D., Kale D.S., Sonwane A., Patil D.V., Jawale M.R., Kharkar K.P. (2020). PCR- RFLP Polymorphism at Exon 3 and Promoter Region of Prolactin Gene in Gaolao Cattle. Indian Journal of Animal Research. 54(5): 523-528. doi: 10.18805/ijar.B-3745.
The present study was planned to investigate significant polymorphism in exon3 and promoter regions of prolactin gene in Gaolao cattle and its association (if any) with milk components. A total of 50 randomly selected Gaolao cows were genotyped for PRLGI-RsaI and PRLGIII-Tsp45I loci using PCR-RFLP technique. The PRLGI-RsaI locus was polymorphic and PRLGIII-Tsp45I locus was monomorphic indicating genetic variation at PRLGI-RsaI locus in Gaolao cattle. The allele frequency for the PRLA alleles was found 0.933 and for allele PRLB was 0.067 at PRLGI–RsaI locus. The result of analysis variance showed significant (PÂ0.05) effect of AA genotype on fat % as compared to AB genotype; however non-significant effect of these genotypes was found on protein %, SNF % and lactose % which may be due to small sample resulting in higher error variance. The results of present study indicated that identified prolactin gene polymorphism at PRLGI-RsaI in exon 3 after validation in large breeding populations having precise data records may prove as important candidate gene marker for selection and breeding decisions in genetic improvement of indigenous cattle for milk productivity. 
  1. Alfonso, E., Rojas, R., Herrera, J.G., Ortega, M.E., Lemus, C., Cortez, E., Ruiz, J., Pinto, R., Gómez, H. (2012). Polymorphism of the Prolactin gene (PRL) and its relationship with milk production in American Swiss cattle. African Journal of Biotechnology. 11(29): 7338-7343.
  2. Barendse, W., Vaiman, D., Kemp, S.J., Sugimoto, Y., Armitage, S.M., Williams, J.L., Sun, A. (1997). Medium-density genetic linkage map of the bovine genome. Mamm. Genome. 8: 21-28.
  3. Bilal, A. and Cinar, M.U. (2014). Analysis of Prolactin and kappa-casein genes polymorphism in four cattle breeds in Turkey. Ann. Anim. Sci., 14(4): 799–806.
  4. Bukhari, S., Khan, N.N., Gupta, P., Das, A.K., Raher, G.A., Chakraborty, D., Pandey, A. (2013). Prolactin gene polymorphism and its associations with milk production traits in Frieswal cow. Intl. J. of Molecular Zoology. 3(3): 10-13.
  5. Camper, S.A., Luck, D.N., Yao, Y., Woychik, R.P., Goodwin, R.G., Lyons, R.H., Rottman, F.M. (1984). Characterization of the Bovine Prolactin Gene. DNA, 3:237-249.
  6. Chung, E.R., Rhim, T.J., Han, S.K. (1996). Association PCR-RFLP markers of growth hormone and Prolactin genes and production traits in dairy cattle. Korean J Ani Sci., 38: 321-336.
  7. Das, N.D., Hatkar, D.N., Sri Hari, V.G., Srinivas, B.V., Kaliaperumal, R., Reddy, O.A., Krishnamurthy, L. (2012). Genetic polymorphisms of exons 3 and 4 of Prolactin (PRL) gene in Deoni cattle breed and their association with milk production traits. Int. J. Livest. Res. 2(3): 120-126.
  8. Devlin, B. and Risch, N. (1995). A comparison of linkage disequilibrium measures for fine-scale mapping. Genomics. 29: 311–322.
  9. Dybus, A. (2002). Associations of growth hormone (Gh) and prolactin (Prl) genes polymorphisms with milk production traits in polish black-and-white cattle. Anim. Sci. Papers Reports. 20: 203-212.
  10. Edy, A., Reyna, R., Jose, G.H., Maria, E.O., Clemente, L., Cesar, C., Jaime, R., Rene, P., Heriberto, G. (2012). Polymorphism of the Prolactin gene (PRL) and its relationship with milk production in American Swiss cattle. African Journal of Biotechnology. 11(29): 7338-7343.
  11. Ghasemi, N., Mohammad, Z., Rahimi, G., Hafezian, S.H. (2009). Associations between Prolactin gene polymorphism and milk production in montebeliard cows. International Journal of Genetics and Molecular Biology. 1(3): 048-051.
  12. Karuthadurai, T.,Chakravarthy, A.K., Kumaresan, A., Kour Aneet., Pradeep Nag, B.S., Rana Ekta., Ravikumar, D. and Yousuf Saleem (2019). Identification of genetic marker for prolactin gene related to milk yield in pedigreed Sahiwal population. Indian Journal of Animal Research. 53 (5): 566-571.
  13. Khatami, S.R., Lazebny, O.E., Maksimenko, V.F., Sulimova, G.E. (2005). Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and Black-and-White Cattle. Russian Journal of Genetics. 41(2): 167–173. 
  14. Kumari, A.R., Singh, K.M., Soni, K.J., Patel, R.K., Chauhan, J.B., Rao, S.K. (2008).Genotyping of the polymorphism within exon 3 of prolactin gene in various dairy breeds by PCR RFLP.Arch. Tierz. Dummerstorf. 5(3):298-299.
  15. Leprovost, F., Leroux, C., Martin, P., Gaye, P., Dijane, J. (1994). Prolactin Gene Expression in Ovine and Caprine Mammary Gland. Neuroendocrinology. 60: 305- 313.
  16. Lewin, H.A., Schmitt, K., Hubert, R., Van Eijk, M.J., Arnheim, N. (1992). Close linkage between bovine Prolactin and BoLADRB3 genes: genetic mapping in cattle by single sperm typing. Genomics. 13: 44-48.
  17. Masoud, A., Kalashnikova, L., Rodionov, G. (2007). Association of Prolactin gene variants with milk production traits in Russian Red Pied cattle.Iranian Journal of Biotechnology. 5(3): 158-161.
  18. Mitra, A., Schlee, P., Balakrishnan, C.R., Pirchner, E. (1995). Polymorphisms at growth hormone and Prolactin loci in Indian cattle and buffalo. J Anim Breed Genet. 112: 71-74.
  19. Nielsen, D.M., Ehm, M.G., Weir, B.S. (1998). Detecting marker-disease association by testing for Hardy-Weinberg Disequilibrium at a Marker Locus. A.J.H.G. 63(5): 1531-40.
  20. Ogorevc, J., Kunej, T., Razpet, A., Dovc, P. (2009). Database of cattle candidate genes and genetic markers for milk production and mastitis. Animal Genetics. 40(6): 832–851. 
  21. Paramitasari, K.A., Sumantri, C., Jakaria (2015). The genetic variability of Prolactin and signal transducers and Activators of Transcription 5A (STAT5A) Genes in Bali Cattle. Media Peternakan. 38(1): 1-11.
  22. Patel, J.B. and Chauhan, J.B. (2017).Polymorphism of the Prolactin gene and its relationship with milk production in Gir and Kankrej Cattle. J. Nat. Sc. Biol. Med. 8: 167-70.
  23. Singh, U., Deb, R., Kumar, S., Singh, R., Sengar, G., Sharma, A. (2015). Association of Prolactin and beta-lactoglobulin genes with milk production traits and somatic cell count among Indian Frieswal (HF 3 Sahiwal) cows. Biomarkers and Genomic Medicine. 7: 38-42.
  24. Sodhi, M., Mukesh, M., Mishra, B.P., Parvesh, K., Joshi, B.K. (2011). Analysis of genetic variation at the Prolactin-RsaI (PRL-RsaI) locus in Indian native cattle breeds (Bos indicus). Biochem. Genet. 49(1-2): 39-45.
  25. Sonmez, Z. and Ozdemir, M. (2017). Prolactin-RsaI gene polymorphism in East Anatolian Red cattle in Turkey. South African Journal of Animal Science. 47(2): 124-129.
  26. Thuy, N.T.D., Thua N.T., Cuonga, N.H., Tya, L.V., Nguyenb, T.T.B., Khoa, D.V.A. (2018). Polymorphism of PIT-1 and Prolactin genes and their effects on milk yield in Holstein Frisian Dairy Cows Bred in Vietnam. Russian Journal of Genetics. 3(54):346–352.
  27. Unal, E.O., Kepenek, E.S., Dinc, H., Ozer, F., Sonmez, G., Togan, Ý.Z., Soysal M. Ý. (2015). Growth hormone (GH), Prolactin (PRL) and diacylglycerol acyltransferase (DGAT1) gene polymorphisms in Turkish native cattle breeds. Turk. J. Zool. 39: 734-748.
  28. Wallis, M. (1974). The primary structure of bovine Prolactin. FEBS Lett. 44: 205-208.
  29. Yeh, F.C. and Boyle, T.J.B. (1997). Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian Journal of Botany. 129: 157-163.

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