Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 54 issue 3 (march 2020) : 300-304

F Gene and N Gene based Reverse Transcription PCR for Molecular Characterization of Peste des Petits Ruminants Virus

A.K. Pandey, D.M. Muglikar, P.P. Mhase, M.M. Pawade, S.N. Daphal, P.D. Pawar
1Department of Veterinary Microbiology, Krantisinh Nana Patil  College of Veterinary Science, Shirwal- 412 801, Maharashtra, India.
Cite article:- Pandey A.K., Muglikar D.M., Mhase P.P., Pawade M.M., Daphal S.N., Pawar P.D. (2020). F Gene and N Gene based Reverse Transcription PCR for Molecular Characterization of Peste des Petits Ruminants Virus. Indian Journal of Animal Research. 54(3): 300-304. doi: 10.18805/ijar.B-3727.
Present investigation was undertaken to detect and characterize the PPR virus from different clinical tissue samples of 14 sheep and 17 goats with respiratory disease from Maharashtra, India. All animals were tested by Sandwich ELISA, of which 70.96% were found positive carrying high PPR virus inwhich 12 were sheep and 10 were goats respectively. For confirmation of PPR, molecular detection was performed with RT-PCR using F gene and N gene specific primers. Intestine samples accounted for highest percent positivity (75%) followed by blood (66.66%) and lymph node (62.5%) for presence of virus. Unusually higher positivity was observed in heart, liver and Kidney (60%, each) than normal predilection sites such as lungs (57.84%) and spleen (50%). While nasal swabs and blood were individually processed with F gene and N gene specific PCR, the triturated organs were pooled for processing into ‘Sample A’ comprised of heart, kidney, liver and intestine combined together and ‘Sample B’ comprising of lung, spleen and lymph nodes combined for the molecular detection of PPR yielding the products each of 372bp and 463bp sizes, respectively. Out of total 40 samples tested, 09/12 each from both sample A and B, while 02/10 nasal swabs resulted positive, respectively and all 06 blood samples remained negative. (F as well as N gene PCR methods were found best suitable for detection of PPR virus from tissue samples of small ruminants). 
  1. Abu Elzein, E.M., Hassanien, M.M., Al-Afaleq, A.I., AbdElhadi, M.A. and Housawi, F. M. (1990). Isolation of peste des petits ruminants from goats in Saudi Arabia. The Veterinary Record. 127(12): 309-10. 
  2. Balamurugan, V., Krishnamoorthy, P., Raju, D.S.N., Rajak, K.K., Bhanuprakash, V., Pandey, AB.and Rahman, H. (2014). Prevalence of Peste-des- petits ruminant virus antibodies in cattle, buffaloes, sheep and goats in India. Virus Disease, 25(1): 85–90.
  3. Balamurugan, Vinayagamurthy, Sen, A., Venkatesan, G., Bhanot, V. and Yadav, V. (2012a). Science Peste des petits ruminants virus detected in tissues from an Asiatic lion (Pantherale-
  4. -opersica) belongs to Asian lineage IV. Journal of Veterinary Science, 13(2): 203-206.
  5. Bhaskar, S.R., Deshmukh, V.V., Chopade N.A., Rautmare, S.S. (2009). Seroprevalence of Peste des petits ruminants in Maharashtra. Indian Journal of Animal Research. 43(4): 285-287.
  6. Bhaskar, S.R., Deshmukh, V.V., Chopade, N.A., Rautmare, S.S. and Aziz, A. (2011). Peste Des Petits Ruminants (PPR) outbreak in sheep and goats in Maharashtra laboratory confirmation by s-ELISA (Mukteshwar) and vero cell culture. Animal Science Reporter. 5(2): 64–68.
  7. Choudhary Sharda, Ahlawat S.P.S, Gupta S.C. and Gupta Neelam (2013). Determination of prp locus snps in garole sheep breed for scrapie. 47(2): 147-151.
  8. Chavan, V.V., Digraskar, S.U., Dhonde, S.N., and Bedarkar, S.N. (2009). Seromonitoring of Peste Des Petits ruminants (PPR) in goats ( Capra hircus ) of Parbhani region of Maharashtra. Veterinary World. 2(8): 299–300.
  9. Dhar, P., Sreenivasaan, B.P., Barrett, T., Corteyn, M., Singh, R.P. and Bandyopadhya, S.K. (2002). Recent epidemiology of peste des petitsruminants virus. Veterinary Microbiology. 88(2): 153–159.
  10. Forsyth, M.A. and Barrett, T. (1995). Evaluation of polymerase chain reaction for the detection and characterisation of rinderpest and peste des petitsruminants viruses for epidemiological studies. Virus Research. 39(2-3): 151–63.
  11. Kataria A., NaliniKataria, K. and Gehlot, A.K. (2007). Large scale outbreaks of PPR in Sheep and Goats in Thar Desert. Slov Veterinary Research. 44(4):123- 32.
  12. Kerur, N., Jhala, M.K. and Joshi, C.G. (2008). Genetic characterization of Indian peste des petits ruminants virus (PPRV) by sequencing and phylogenetic analysis of fusion protein and nucleoprotein gene segments. Research in Veterinary Science. 85(2008): 176–183.
  13. Khan H.A., Sajjad-ur -Rahman, A.K. and Q. Ser M.K. (2013). Detection and sequencing of field isolates of peste des petits ruminants virus from Punjab Province, Pakistan. Pakistan Journal of Life and Social Science. 11(3): 212-217
  14. Luka, P.D., Erume, J., Mwiine, F.N., Ayebazibwe, C. and Shamaki, D. (2011). Molecular characterization and phylogenetic study of peste des petits ruminants viruses from North central States of Nigeria. BMC Veterinary Research. 7(1): 32.
  15. Mahajan, S., Agrawal, R., Kumar, M., Mohan, A. and Pande, N. (2013). Incidence of peste des petits ruminants in nomadic sheep and goat of Jammu region. Veterinary World. 6(7): 384–387.
  16. Manimaran, K., Selvaraj J., Jaisree S., Aravindh Babu R.P., Hemalatha S., Raja A. and Roy Parimal (2017). An outbreak of peste des petitis ruminants in sheep and goats at Salem district of Tamil Nadu, India. Indian Journal of Animal Research. 51 (2): 332-335.
  17. Munir, M. (2012). Peste des Petits Ruminants Virus, IstEdn, 65–    98 Springer Publications.
  18. Raj, G.D., Kumar, A.S.S., Shaila, M.S., Nachimuthu, K. and Palaniswami, K.S. (2003). Molecular epidemiology of peste des petitsruminants viruses from southern India. Veterinary Record. doi:10.1136/vr.152.9.264
  19. Shaila, M.S.; Purashothaman, V.; Bhavsar, D.; Venugopal, K. and Venkatesan, R.A. (1989). Peste des petits ruminants of sheep in India. Veterinary Research. 125: 602.
  20. Shaila, M.S.; Shamaki, D.; Forsyth, M.A.; Diallo, A.; Goatley, L.; Kitching, R.P. and Barrett, T. (1996). Geographic distribution and epidemiology of peste des petitsruminants virus. Virus Research. 43 (2):149-53.
  21. Tiwari, A. (2004). Prevalence of Peste des petits ruminants (PPR) virus in small ruminants of Gujarat and its characterization by RT- PCR/ RFLP and SSCP profiles.M.V.Sc. thesis submitted to Anand Agricultural University, Anand, India.

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