Phenotypic and genotypic characterization of mecA and blaZ gene mediated antibiotic resistance in bovine mastitis due to Staphylococcus spp.

DOI: 10.18805/ijar.B-3726    | Article Id: B-3726 | Page : 125-127
Citation :- Phenotypic and genotypic characterization of mecA and blaZ gene mediated antibiotic resistance in bovine mastitis due to Staphylococcus spp..Indian Journal Of Animal Research.2020.(54):125-127
Jibin Jose, Surya Sankar, Binu K. Mani, M.P. Unnikrishnan, Anu Bosewell and M. Mini surya@kvasu.ac.in
Address : Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy Kerala Veterinary and Animal Sciences University, Pookode, Wayanad-673 576, Kerala, India.
Submitted Date : 7-09-2018
Accepted Date : 30-01-2019

Abstract

A total of thirty milk samples from bovine clinical mastitis are subjected to cultural isolation followed by antibiogram of the isolates. All the isolates obtained were resistant to methicillin and penicillin and most of them showed resistance to other beta lactam group of antibiotics. Out of the thirteen Staphylococcus spp. isolated, ten were coagulase negative and all showed positive amplicons for the presence of blaZ gene in polymerase chain reaction. Ten isolates showed positive results for the presence of mecA gene.

Keywords

Bovine mastitis blaZ mecA PCR

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