Histochemical changes during development of Harderian gland in chicken

DOI: 10.18805/ijar.B-3723    | Article Id: B-3723 | Page : 41-46
Citation :- Histochemical changes during development of Harderian gland in chicken.Indian Journal Of Animal Research.2020.(54):41-46
Jigyasa Rana, S.B. Banubakode, N.C. Nandeshwar, N.V. Kurkure, J.P. Korde and S.K. Patel rana.jigyasa@gmail.com
Address : Department of Veterinary Anatomy and Histology, Nagpur Veterinary College, Seminary Hills, Nagpur-440 006, Maharashtra, India.
Submitted Date : 4-09-2018
Accepted Date : 31-10-2018

Abstract

The present study was aimed to examine histochemical changes in the Harderian gland of pre and post hatched chicks from 11 day of incubation to 24 days of age. In pre hatched groups, the gland was in the developing stage. There was no well defined parenchyma, septa and capsule in early embryonic stage. Histochemical observation revealed that weak or negligible activity of PAS, AB/PAS and Sudan black B was seen in epithelial lining of Harderian gland on 18th and 20th day of incubation. The gland was compound tubule-acinar type at 20th day of incubation, consisted of capsule, parenchyma and stroma. PAS activity was seen in the capsule, septa, epithelial lining of acini and tubule and interstitial connective tissue cells. PAS activity was aggregated towards the apical part of the tubular epithelium as well as at the free luminal surface epithelium of the acini. PAS activity increases with the development of connective tissue component of Harderian gland with the advancement of age. The Harderian gland was predominantly mucus and lipid secreting gland. All epithelial cells of the glandular unit of Harderian gland contain both neutral and acidic mucin. Intense AB-positive activity was seen at the apex of tubule with the advancement of age. Sudan black B activity was recorded in the cytoplasm of epithelial lining of tubule and acini of the Harderian gland and their presence increases with age. 

Keywords

Chicken Harderian gland Histochemical Lipid Mucopolysaccharides Plasma cells.

References

  1. Baba, T., Kawata, T., Masumoto, K. and Kajikawa, T. (1990). Role of the Harderian gland in immunoglobulin A production in chicken lacrimal fluid. Res. Vet. Sci., 49: 20-24.
  2. Bancroft, J.D., Stevens, A. and Turner, D.R. (1996). Theory and Practice of Histological Techniques, 2nd ed. Churchill Living Stone, Edinburgh, London, Mlourna and New York.
  3. Bejdic, Pamela, Avdic, Rizah, Amidzic, Ljiljana, Cutahija, Velida, Tandir, Faruk and Hadziomerovic, Nedzad (2014). Developmental changes of lymphoid tissue in the Harderian gland of laying hens. Mac. Vet. Rev., 37(1): 83-88.
  4. Boydak, M. and Aydin, M.F. (2009). Histology of the Harderian gland of domestic geese (Anser domesticus). Acta. Vet. Brno., 78: 199-204.
  5. Burns, R.B. and Maxwell, M.H. (1979). The structure of the Harderian and lacrimal gland ducts of the turkey, fowl and duck. A light microscope study. Journal of Anatomy, 128(2): 285-292.
  6. El-leithy, Ebtihal M.M.H. (2015). Studies on the Harderian gland of the rabbit. Ph.D. thesis submitted to Cairo University. 
  7. Fix, A.S. and Arp, L.H. (1991). Morphologic characterization of conjunctiva associated lymphoid tissue in chicken. Am. J. Vet. Res., 52: 1852–1859.
  8. Frahmand, S. and Mohammadpour, A.A. (2015). Harderian gland in Canadian ostrich (Struthio camelus): a morphological and histochemical study. Anatomia Histologia Embryologia, 44(3): 178-185.
  9. Hussein, Ola A., Elgamal, Dalia A. and Elgayar, Sanaa A.M. (2015). Structure of the secretory cells of male and female adult guinea pigs Harderian gland. Tissue and Cell, 47: 323-335. 
  10. Khan, M.Z.I., Jahan, M.R., Islam, M.N., Haque, Z., Islam, M.R. and Kon, Y. (2007). Immunoglobulin (Ig)-containing plasma cells in the Harderian gland in broiler and native chickens of Bangladesh. Tissue and Cell, 39: 141-149.
  11. Kozlu, T. and Altunay, H. (2011). Light and electron microscopic studies of the quail (Coturnix coturnix) Harderian Gland. Journal of Animal and Veterinary Advances, 10(7): 932-938.
  12. Kozlu, T., Bozkurt, Y.A., Altunay, H. and Sari, E.K. (2010). Histological and histochemical studies on the Harderian gland of the osprey (Pandion haliaetus). Journal of Animal and Veterinary Advances, 9(13): 1875-1879.
  13. Luna, L.G. (1972). Manual of Histological Staining Methods of the Armed Forces Institute of Pathology. The Blakiston Division, McGraw Hill Book Co. N.Y. pp. 162-164.
  14. Mobini, B. (2012). Histological and histochemical studies on the Harderian gland in native chickens. Veterinarni Medicina, 57(8): 404–409.
  15. Mobini, B. (2014). Histological and histochemical studies on the paraorbital gland in broilers. J. Anim. Pro. Adv., 4(11): 527-533.
  16. Pawar, A., Venkatkrishnan, A., Vijayaragavan, C. and Ramkrishna, V. (1998). Histological study of the Harderian gland of White Leghorn with reference to plasma cells. Indian Journal of Animal Sciences, 68: 141-142.
  17. Payne, A.P. (1994). The Harderian gland: a tercentennial review. Journal of Anatomy, 185: 1-49.
  18. Reshag, Ali F., Hadi, Ilaf Hassan and Mohammed, Hadaf H. (2016). Morphological and histochemical study of Harderian gland of domestic pigeon (Columba livia domestica). Bull. Iraq nat. Hist. Mus., 14(2): 161-170.
  19. Shirama, K., Satoh, T., Kitamura, T. and Yamada, J. (1996). The avian Harderian gland: morphology and immunology. Microsc. Res. Techniq., 34: 16-27. 
  20. Wight, P.A.L., Burns, R.B., Rothwell, B. and Macken, G.M. (1971). The Harderian glands of the domestic fowl II.Histochemistry. Journal of Anatomy, 110(3): 323-333.

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