Effect of additives in medium on in-vitro maturation of goat oocytes

DOI: 10.18805/ijar.B-3722    | Article Id: B-3722 | Page : 11-15
Citation :- Effect of additives in medium on in-vitro maturation of goat oocytes.Indian Journal Of Animal Research.2020.(54):11-15
D. Borah and R.K. Biswas drdharitriborah@gmail.com
Address : Department of Animal Reproduction, Gynaecology and Obstetrics College of Veterinary Science, Assam Agricultural University, Khanapara-781 022, Assam, India.
Submitted Date : 1-09-2018
Accepted Date : 15-12-2018

Abstract

Present study was carried out to find the effect of combining EGF with IGF, cysteine and sodium pyruvate singly as additive in a medium consisting of TCM-199 + 100 µl/ml foetal bovine serum + 100 µM/ml cysteamine + 1 µg/ml 17â- Oestradiol + 5 µg/ml pFSH + 5µg/ml oLH + 10 per cent follicular fluid and 10 per cent oestrous goat serum on in-vitro maturation (IVM) of caprine oocytes on incubation at 38.50C for 24 hours in a CO2 incubator maintaining 5 per cent CO2 under humidified condition. The additives comprised 10 ng/ml EGF + 50 ng/ml IGF-1, 10 ng/ml EGF + 600 µM/ml cysteine and 10 ng/ ml EGF + 0.2 mM/ml sodium pyruvate. The IVM rate of oocytes on the basis of cumulus cells expansion and nuclear maturation was found to be significantly higher (P<0.05) with EGF + IGF-1 (88.74 ± 1.85% and 61.71 ± 1.61%) than with EGF + sodium pyruvate (82.86 ± 0.97% and 54.62 ± 1.88%), EGF + cysteine ( 78.58 ± 1.45% and 49.02 ± 1.52%) and without additive (control) (75.27 ± 1.58% and 43.03 ± 1.48%).

Keywords

Caprine oocytes Cumulus cells expansion In-vitro maturation Nuclear maturation.

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