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Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Research Article

Indian Journal of Animal Research, volume 53 issue 12 (december 2019) : 1645-1650

Molecular typing of Indian Mycoplasma gallisepticum isolates

Susitha Rajkumar, Maddula Ramkoti Reddy, Ramesh Somvanshi
1Avian Health Lab, ICAR-Directorate of Poultry Research, Hyderabad-500 030, Telangana, India.
Cite article:- Rajkumar Susitha, Reddy Ramkoti Maddula, Somvanshi Ramesh (2019). Molecular typing of Indian Mycoplasma gallisepticum isolates. Indian Journal of Animal Research. 53(12): 1645-1650. doi: 10.18805/ijar.B-3715.

ABSTRACT

M. gallisepticum infection is a serious economic threat to the poultry industry in India, and the genetic diversity of Indian isolates is unknown. Gene-targeted sequence analysis of M. gallisepticum was conducted for the first time to map the diversity among different Indian isolates from different regions and from different clinical conditions. Sequence analysis of the mgc2 gene grouped the 13 field isolates into four groups. The most prevalent sequence type, the group1 shared 100% identity with the foreign strains THA 3CK08 (Thailand) and UHP1CK99 (Israel). Groups two and three were novel, and were genetically distinct from the strains outside the country. Group four shared 100% similarity with the vaccine strain MG6/85. The nucleotide similarity between the 4 groups ranged from 94.3-99.2%. A PCR-RFLP method using HaeII was tested and validated for its ability to differentiate the Indian field isolates from vaccine strains.

REFERENCES

  1. Chakraborty, D., Sadhukahan, T., Guha, D.and Chatterjee, A. (2001) Seroprevalence of Mycoplasma gallisepticum in West Bengal. Indian Vet J.78: 855-856.
  2. Delaney, N.F., Balenger, S., Bonneaud, C., Marx, C.J., Hill, G.E., Ferguson-Noel, N., Tsai, P., Rodrigo, A. and Edwards, S.V. (2012) Ultrafast evolution and loss of CRISPRs following a host shift in a novel wildlife pathogen, Mycoplasma gallisepticum. PLoS Genet.8: e1002511. doi: 10.1371/journal.pgen.1002511
  3. El Gazzar, M., Laibinis, V.A. and Ferguson-Noel, N. (2011) Characterization of a ts-1-like Mycoplasma galisepticum isolate from commercial broiler chickens. Avian Dis.55: 569-574. 
  4. Fabricant, J. and Levine, P.P (1962) Experimental production of complicated chronic respiratory disease infection. Avian Dis.6: 13-23.
  5. Felsenstein, J. (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39: 783-791.
  6. Ferguson, N.M., Hepp, D., Sun, S., Ikuta, N., Levisohn, S., Kleven, S.H. and Garcia, M. (2005) Use of molecular diversity of Mycoplasma gallisepticum by gene-targeted sequencing (GTS) and random amplified polymorphic DNA (RAPD) analysis for epidemiological    studies. Microbiol.151: 1883-1893. 
  7. Gharaibeh, S., Laibinis, V., Wooten, R., Stabler, L. and Ferguson-Noel, N. (2011) Molecular characterization of Mycoplasma gallisepticum    isolates from Jordan. Avian Dis. 55: 212-216. 
  8. Ghorashi, S., Noormohammadi, A. and Markham, P. (2010) Differentiation of Mycoplasma gallisepticum strains using PCR and high-    resolution melting curve analysis. Microbiol.156: 1019-1029.
  9. Gross, W. (1961) The development of “air sac disease”. Avian Dis.5: 431-439.
  10. Heldtander, M., Wesonga, H., Bolske, G., Pettersson, B. and Johansson, K.E. (2001) Genetic diversity and evolution of Mycoplasma capricolum subsp. capripneumoniae strains from eastern Africa assessed by 16S rDNA sequence analysis. VetMicrobiol. 78: 13-28.
  11. International Office of Epizootics, B.S.C. and Committee, I.O.o.E.I. 2008. “Avian Mycoplasmosis (M. gallisepticum and M. synoviae) “ In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals: mammals, birds and bees, pp.482-496. Office international des épizooties.
  12. Khalifa, R., Eissa, S., El-Hariri, M. and Refai, M. (2014) Sequencing analysis of Mycoplasma gallisepticum wild strains in vaccinated chicken breeder flocks. Journal of Molecular Microbiol Biotechnol.24: 98-104.
  13. Kleven, S.H. (1998) Mycoplasmas in the etiology of multifactorial respiratory disease. Poultry Science, 77: 1146-1149. 
  14. Kleven, S.H., Fulton, R.M., Garcia, M., Ikuta, V.N., Leiting, V.A., Liu, T., Ley, D.H., Opengart, K.N., Rowland, G.N. and Wallner-    Pendleton, E. (2004) Molecular characterization of Mycoplasma gallisepticum isolates from turkeys. Avian Dis. 48: 562-569. 
  15. Kumar, S., Stecher, G. and Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol. 33: 1870-1874.
  16. Ley, D.H. (2008). “Mycoplasma gallisepticum infection.” In Diseases of Poultry, edited by Y M Saif, A.M Fadly, J.R Glisson, L.R McDougald, L.K. Nolan and D.E Swayne, Blackwell Publishing Professional. Ames, Iowa, USA: pp. 807-834.
  17. Lysnyansky, I., Garcia, M. and Levisohn, S. (2005) Use of mgc2-polymerase chain reaction-restriction fragment length polymorphism for rapid differentiation between field isolates and vaccine strains of Mycoplasma gallisepticum in Israel. Avian Dis. 49: 238-245. 
  18. Mohammed, H.O., Carpenter, T.E. and Yamamoto, R. (1987) Economic impact of Mycoplasma gallisepticum and M. synoviae in commercial layer flocks. Avian Dis. 31: 477-482.
  19. Rajkumar, S., Reddy, M.R. and Somvanshi, R. (2017) Molecular detection and pathology of spontaneous cases of Chronic Respiratory Disease in chicken. Indian J Vet Pathol. 41: 277-282.
  20. Rajkumar, S., Reddy, M.R., and Somvanshi, R. (2018). Molecular prevalence and seroprevalence of Mycoplasma gallisepticum and M. synoviae in Indian poultry flocks. Journal of Animal Research, 8: 15-19.
  21. Ramadass, R., Ananthi, Senthil Kumar, T., Venkatesh, G. and Ramaswamy, V. (2006) Isolation and characterization of Mycoplasma gallisepticum and Mycoplasma synoviae from poultry. Indian J AnimSci. 76: 796-798.
  22. Saha, T., Guha, C., Biswas, U., Chakraborty, D., Sadhukhan, T. and Chakraborty, G. (2011) Isolation and identification of Mycoplasmas from respiratory disease affected broiler birds in West Bengal. Indian J AnimSci.81: 1124-1125.
  23. Soeripto, Whithear, K.G., Cottew, G.S. and Harrigan, K.E. (1989) Virulence and transmissibility of Mycoplasma gallisepticum. AustVetJ. 66: 65-72.
  24. Stipkovits, L. and Kempf, I. (1996) Mycoplasmoses in poultry. Rev SciTech. 15: 1495-1525.
  25. Tamura, K. and Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 10: 512-526.
  26. Throne Steinlage, S.J., Ferguson, N., Sander, J.E., Garcia, M., Subramanian, S., Leiting, V.A. and Kleven, S.H. (2003) Isolation and characterization of a 6/85-like Mycoplasma gallisepticum from commercial laying hens. Avian Dis. 47: 499-505. 
  27. Udhayavel, S., Murthy, T.R.G.K., Gowthaman, V., Senthilvel, K. and Sureshkumar, G. (2016) Detection of sub clinical infection of Mycoplasma gallisepticum in commercial chicken by indirect ELISA. AdvAnimVetSci.4: 438-440.
  28. Whithear, K.G. (1996) Control of avian mycoplasmoses by vaccination. Rev SciTech.15: 1527-1553.
  29. Woese, C.R., Stackebrandt, E. and Ludwig, W. (1984) What are mycoplasmas: the relationship of tempo and mode in bacterial evolution. JMolEvol. 21: 305-316.
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