Molecular analysis of oncogenicity associated gene “vIL8” of serotype 1 Marek’s disease virus isolates from India

DOI: 10.18805/ijar.B-3693    | Article Id: B-3693 | Page : 83-89
Citation :- Molecular analysis of oncogenicity associated gene “vIL8” of serotype 1 Marek’s disease virus isolates from India.Indian Journal Of Animal Research.2020.(54):83-89
P. Suresh, J. Johnson Rajeswar, K. Sukumar, T.J. Harikrishnan and P. Srinivasan
Address : Department of Veterinary Microbiology,Veterinary College and Research Institute, Namakkal-637 002, Tamilnadu Veterinary and Animal Sciences University, Tamil Nadu, India.
Submitted Date : 19-07-2018
Accepted Date : 10-06-2019


This study was undertaken to characterize the oncogenicity associated vIL8 (viral interleukin-8) gene at molecular level for three serotype 1 Marek’s Disease Virus (MDV) field isolates recovered from vaccinated poultry flocks. The prime aim was to generate nucleotide sequence data for important oncogenicity associated vIL8 gene of MDV1 which is very much lacking in India. Eighty six blood samples were collected from 15 commercial layer and broiler breeder farms. Isolation was done in duck embryo fibroblasts (DEF) and vIL8 gene was amplified by Polymerization Chain Reaction (PCR) and sequenced. The isolates were shown to have a homology of 99.8-100 per cent within themselves and 94.6-95.1 per cent with various isolates of China and 96.6 – 99.5 per cent with other isolates of USA for vIL8 gene. Alignment analysis of the nucleotide sequences had shown nucleotide mutations at three different positions and displayed perfect regularity. Phylogenetic analysis of vIL8 sequence of three isolates and other six reference strains showed that the analyzed nine MDVs could be distinctly separated into two clusters. The study revealed that the field isolates of serotype 1 MDVs circulating in vaccinated flocks had been shown to have consistent mutations in different positions in vIL8 gene.


Alignment analysis Chemokine Isolation Marek’s disease Nucleotide sequence


  1. Cui, Z.Z., Lee L.F., Liu J.L., and Kung H.J. (1991). Structural analysis and transcriptional mapping of the Marek’s disease virus gene encoding pp38, an antigen associated with transformed cells. J Virol. 65: 6509-515.
  2. Cui, X., Lee L.F., Reed W.M., Kung H.J., and Reddy S.M. (2004). Marek’s disease virus- encoded vIL8 gene is involved in early cytolytic infection but dispensable for establishment of latency. J Virol.78: 4753-4760.
  3. Davison, A.J. (2002). Evolution of the herpesviruses. Vet Microbiol. 86: 69-88.
  4. Gong, Z., Zhang L., Wang J., Chen L., Shan H., Wang Z., and Ma H. (2013). Isolation and analysis of a very virulent Marek’s disease virus strain in China. Virol J. 10: 155.
  5. Gopal, S., Manoharan P., Kathaperumal K., Chidambaram B., and Divya K.C. (2012). Differential detection of avian oncogenic viruses in poultry layer farms and turkeys by use of multiplex PCR. J Clin Microbiol. 50(8): 2668 -2673.
  6. Jones, D., Lee L., Liu J.L., Kung H.J., and Tillotson J.K. (1992). Marek’s disease virus encodes a basic-leucine zipper gene resembling the fos/jun oncogenes that is highly expressed in lymphoblastoid tumors. Proceedings of National Academy of Sciences.USA. 89: 4042-4046.
  7. Liu, J.L., Lin S.F., Brunovskis P., Davidson I., Lee L.F. and Kung H.J. (1999). Meq and vIL8: Cellular genes in disguise? Acta Virol. 43: 94-101.
  8. Parcells, M.S., Lin S.F., Dienglewicz R.L., Majerciak V., Robinson D.R., Chen H.C., Wu Z., Dubyak G.R., Brunovskis P., Hunt H.D., Lee L.F. and Kung H.J. (2001). Marek’s disease virus (MDV) encodes an interleukin-8 homolog (vIL8): characterization of the vIL8 protein and a vIL8 deletion mutant MDV. J Virol. 75: 5159-5173.
  9. Peng, Q., and Shirazi Y. (1996). Characterization of the protein product encoded by a splicing variant of the Marek’s disease virus Eco-    Q gene (Meq). Virol. 226: 77-82.
  10. Raja, A., Dhinakar Raj G., Bhuvaneswari P., Balachandran C., and Kumanan K. (2009). Detection of virulent Marek’s disease virus in poultry in India. Acta Virol. 53: 255-260.
  11. Suresh, P., Johnson Rajeswar J., Sukumar K., Harikrishnan T.J., and Srinivasan P. (2013). Incidence of Marek’s disease in vaccinated flocks. Intl J Food Agri Vet Sci. 3: 200-202.
  12. Suresh, P., Johnson Rajeswar J., Sukumar K., Harikrishnan T.J., and Srinivasan P. (2015). Pathotyping of recent Indian field isolates of Marek’s disease virus serotype 1. Acta Virol. 59:156-165.
  13. Suresh, P., Johnson Rajeswar J., Sukumar K., Harikrishnan T.J., and Srinivasan P. (2017). Complete nucleotide sequence analysis of the oncogene “Meq” from serotype 1. British Poultry Science. 58(2): 111-115
  14. Supplemental assay method for titration of cell associated Marek’s disease vaccines of serotypes 1, 2, OR 3. (2005). United States Department of Agriculture, Animal and Plant Health Inspection Service. pp. 1–15.
  15. Tian, M., Zhao Y., Lin Y., Zou N., Liu C., Liu P., and Huang Y. (2011). Comparative analysis of oncogenic genes revealed unique evolutionary features of field Marek’s disease virus prevalent in recent years in China. Virol J. 8:1-11.
  16. Witter, R.L. and Schat K.A. (2003). Marek’s disease. Diseases of Poultry (11th edition). [In: Calnek, B.W.,] Iowa State University Press: Ames., Iowa. pp. 407-465.
  17. Zhang, Y., and Qin A. (2003). Expression of viral interleukin 8 gene of Marek’s disease virus and its biological characterization. Master’s Thesis, Veterinary College, Yanzhou University. 

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