Detection of Mycobacterium avium subsp. paratuberculosis (MAP) from Subclinical Caprine Paratuberculosis Cases of Odisha

DOI: 10.18805/ijar.B-3691    | Article Id: B-3691 | Page : 709-715
Citation :- Detection of Mycobacterium avium subsp. paratuberculosis (MAP) from Subclinical Caprine Paratuberculosis Cases of Odisha.Indian Journal Of Animal Research.2020.(54):709-715
Sangram Biswal, Adya Prakash Rath, Shoor Vir Singh, Niranjana Sahoo, Saurabh Gupta, Manju Singh, Kundan Kumar Chaubey rathadya10@gmail.com
Address : Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana–141 004, Punjab, India.
Submitted Date : 12-07-2018
Accepted Date : 3-12-2019

Abstract

Paratuberculosis is caused by Mycobacterium avium subsp. Paratuberculosis (MAP) and is a chronic, intestinal tract infection in the ruminant sector globally. A total of 122 EDTA mixed blood samples, 121 serum and 16 pooled faecal samples were collected from farms of 4 different districts i.e. Nayagarh, Cuttack, Khordha and Angul and a blind review was conducted at the Animal Health Division, CIRG, Mathura. Microscopic examination of 16 pooled faecal samples revealed +2 reactivity to Acid-Fast Bacilli. All the serum samples were subjected to indirect ELISA. Out of them, 23 (19.01%), 85 (70.25%), shows strongly positive, positive, antibody titre respectively. EDTA blood samples of 23 ELISA-strongly positive were subjected to 413 bp IS900 PCR and 11 (9%) of them were found positive for Mycobacterium avium subsp. Paratuberculosis (MAP). MAP isolates were further subjected to genotyping using 608 bpIS1311 PCR and restriction endonuclease analysis (IS1311 PCR-REA) and 2 (1.64%) of them matched with “Indian Bison Type”. Genotyping of the isolates using IS1311 PCR-REA revealed that goat population of Odisha are primarily infected with “Indian Bison Type” strains.

Keywords

Acid-fast Bacilli Bison Type IS1311 MAP Paratuberculosis

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