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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 10 (october 2019) : 1329-1334,   Doi: 10.18805/ijar.B-3659

Seasonal variations in enzyme histochemistry of accessory sex glands in buffalo bull  

S
Samikshya Sarangi
A
Anuradha Gupta
N
Neelam Bansal
V
Varinder Uppal
1<div style="text-align: justify;">Department of Veterinary, Anatomy College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.</div>
Cite article:- Sarangi Samikshya, Gupta Anuradha, Bansal Neelam, Uppal Varinder (2018). Seasonal variations in enzyme histochemistry of accessory sex glands in buffalo bull . Indian Journal of Animal Research. 53(10): 1329-1334. doi: 10.18805/ijar.B-3659.

ABSTRACT

The present study was conducted on vesicular, prostate and bulbourethral glands of buffalo bullcollected from the abattoir immediately after sacrifice during winter and summer season. The cryostat sections of 10-12 µm thickness were obtained at -23°C and were incubated in different substrates for demonstration of phosphatases, oxidoreductases and estrases. During winter season, the moderate to strong activity of AKPase in acinar cells and ductular epithelium was observed in vesicular, weak in prostate, but the activity was not demonstrated in bulbourethral gland, however the blood vessels showed moderate AKPase reaction in all the glands. A moderate activity of SDH, LDH and G-6-PD was observed in glandular and ductular epithelium of vesicular gland and weak to moderate activity in prostate and bulbourethral glands. The glandular and the ductular epithelium of all the glands studied were strongly positive for NADH and NADPH, whereas a weak activity for NSE was observed. There was decrease in the activity of all the enzymes during summer season which may be related to decrease physiological activity in this season. 

REFERENCES

  1. Anurag (2000).Histomorphological and histochemical study of postnatal changes in the vesicular, bulbourethral and ampullary glands of buffalo.MVSc Thesis, Department of Veterinary Anatomy, College of Veterinary Sciences, Punjab Agricultural University, Ludhiana.
  2. Barka, T. and Anderson, P. J. (1963).Histochemistry, Theory, Practice, and Bibliography. Harper and Row, New York.
  3. Chopra, R. (1998).Post-natal development of the prostate gland in buffalo (Bubalus bubalis): A histomorphochemical study. MVSc Thesis, Punjab Agricultural University, Ludhiana.
  4. Elhamd, A. A. S., Salem, A. O., and Selim, A. A. (2013). Histomorphological studies on the prostate gland of donkey Equus asinus during different seasons. Journal of Histology. Article ID 643287.
  5. Moussa, M. H., Badawy, A. B., Kandil, M. H., and Shahin, Y. M. (1983).Histological and histochemical studies of the accessory genital glands of buffalo bull (Bubalis bubalis). AnatomischerAnzeiger, 153(5) : 429-39.
  6. Nagasailaja, S., Purusotham, G., Pramodkumar, D., and Raghavendar, K. B. P. (2016).Histology and Histochemistry of Seminal Vesicles in Pig (Sus scrofadomestica).Indian Journal of Veterinary Anatomy,28 (1): 55-7.
  7. Pathak, A., Katiyar, R. S., Sharma, D. N., Farooqui, M. M., and Prakash, A.(2012). Gross anatomical, histological and histochemical studies on the postnatal development of the prostate gland of Gaddi goat.International Journal of Morphology,30(2): 731-39.
  8. Pawar, H. S., Saigal, R. P., Roy, K. S., and Singh, J. (1988). Histoenzymic studies on prostate gland of goat. Indian journal of Animal sciences, 58 : 350-52. 
  9. Pawar, H. S., Roy, K. S.,Saigal, R. P. (1986). Histochemical localization of carbohydrates, lipids and enzymes in bulbourethral gland of ram. Indian Journal of Animal Sciences,56:187- 9.
  10. Pearse, A. G. E. (1972).Histochemistry : Theoretical and Applied. 3rd Edn.pp 607.Churchill Livingstone, London.
  11. Pyne, S. K., Sinha, R. D., Chauhan, H. V. S. (1991).Histochemical study on the bulbo-urethral gland of normal and vasectomised goats (Capra hircus).Indian Veterinary Journal,68 : 145-47.
  12. Rahman, M. S., Islam, M. S., Rahman, M. T., Parvez, N. H., and Rahman, M.M. (2010). Morphometric analysis of vesicular glands of indigenous bull.International Journal of Sustainable Crop Production,5(1):11-4.
  13. Roy, K. S., Pawar, H. S., and Saigal, R. P. (1985). Histomorphological, histochemical and histoenzymological studies on prostate gland of ram (Ovisaries). Indian Journal of Animal Sciences, 55(12): 983-86.
  14. Roy, K. S., Pawar, H. S., and Saigal, R. P. (1987). Observations on seminal vesicle of ram (Ovisaries) a histochemical study. Indian Veterinary Journal, 64: 207-9.
  15. Scala, G. and Maruccio, L. (2012). Nitric oxide (NO) expression during annual reproductive activity in buffalo epididymis: A histochemical and immunocytochemical study. Theriogenology, 78 : 49–56
  16. Selvinjose, S., Basha, S. H., Balasubramanian, and Ramesh, G.(2009). Histochemical studies on the seminal vesicle in Madras red sheep (Ovisaries). Indian Journal of Veterinary Anatomy, 21(2): 5-8.
  17. Sudhakar, L. S. (1982). Histological and histochemical changes in the male accessory genital glands of Murrah buffalo during postnatal development. Ph.D. Thesis. Haryana Agricultural University, Hisar, India.
  18. Suri, S., Sudhakar, L. S., and Bhardwaj, R.L .(2012). Seasonal variation in the histomorphology and histochemistry of the bulbourethral gland of Gaddi goat and gaddi Sheep.Indian Journal of Veterinary Anatomy, 24 (1): 1-4.
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    Research Article

    volume 53 issue 10 (october 2019) : 1329-1334,   Doi: 10.18805/ijar.B-3659

    Seasonal variations in enzyme histochemistry of accessory sex glands in buffalo bull  

    S
    Samikshya Sarangi
    A
    Anuradha Gupta
    N
    Neelam Bansal
    V
    Varinder Uppal
    1<div style="text-align: justify;">Department of Veterinary, Anatomy College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.</div>
    Cite article:- Sarangi Samikshya, Gupta Anuradha, Bansal Neelam, Uppal Varinder (2018). Seasonal variations in enzyme histochemistry of accessory sex glands in buffalo bull . Indian Journal of Animal Research. 53(10): 1329-1334. doi: 10.18805/ijar.B-3659.

    ABSTRACT

    The present study was conducted on vesicular, prostate and bulbourethral glands of buffalo bullcollected from the abattoir immediately after sacrifice during winter and summer season. The cryostat sections of 10-12 µm thickness were obtained at -23°C and were incubated in different substrates for demonstration of phosphatases, oxidoreductases and estrases. During winter season, the moderate to strong activity of AKPase in acinar cells and ductular epithelium was observed in vesicular, weak in prostate, but the activity was not demonstrated in bulbourethral gland, however the blood vessels showed moderate AKPase reaction in all the glands. A moderate activity of SDH, LDH and G-6-PD was observed in glandular and ductular epithelium of vesicular gland and weak to moderate activity in prostate and bulbourethral glands. The glandular and the ductular epithelium of all the glands studied were strongly positive for NADH and NADPH, whereas a weak activity for NSE was observed. There was decrease in the activity of all the enzymes during summer season which may be related to decrease physiological activity in this season. 

    REFERENCES

    1. Anurag (2000).Histomorphological and histochemical study of postnatal changes in the vesicular, bulbourethral and ampullary glands of buffalo.MVSc Thesis, Department of Veterinary Anatomy, College of Veterinary Sciences, Punjab Agricultural University, Ludhiana.
    2. Barka, T. and Anderson, P. J. (1963).Histochemistry, Theory, Practice, and Bibliography. Harper and Row, New York.
    3. Chopra, R. (1998).Post-natal development of the prostate gland in buffalo (Bubalus bubalis): A histomorphochemical study. MVSc Thesis, Punjab Agricultural University, Ludhiana.
    4. Elhamd, A. A. S., Salem, A. O., and Selim, A. A. (2013). Histomorphological studies on the prostate gland of donkey Equus asinus during different seasons. Journal of Histology. Article ID 643287.
    5. Moussa, M. H., Badawy, A. B., Kandil, M. H., and Shahin, Y. M. (1983).Histological and histochemical studies of the accessory genital glands of buffalo bull (Bubalis bubalis). AnatomischerAnzeiger, 153(5) : 429-39.
    6. Nagasailaja, S., Purusotham, G., Pramodkumar, D., and Raghavendar, K. B. P. (2016).Histology and Histochemistry of Seminal Vesicles in Pig (Sus scrofadomestica).Indian Journal of Veterinary Anatomy,28 (1): 55-7.
    7. Pathak, A., Katiyar, R. S., Sharma, D. N., Farooqui, M. M., and Prakash, A.(2012). Gross anatomical, histological and histochemical studies on the postnatal development of the prostate gland of Gaddi goat.International Journal of Morphology,30(2): 731-39.
    8. Pawar, H. S., Saigal, R. P., Roy, K. S., and Singh, J. (1988). Histoenzymic studies on prostate gland of goat. Indian journal of Animal sciences, 58 : 350-52. 
    9. Pawar, H. S., Roy, K. S.,Saigal, R. P. (1986). Histochemical localization of carbohydrates, lipids and enzymes in bulbourethral gland of ram. Indian Journal of Animal Sciences,56:187- 9.
    10. Pearse, A. G. E. (1972).Histochemistry : Theoretical and Applied. 3rd Edn.pp 607.Churchill Livingstone, London.
    11. Pyne, S. K., Sinha, R. D., Chauhan, H. V. S. (1991).Histochemical study on the bulbo-urethral gland of normal and vasectomised goats (Capra hircus).Indian Veterinary Journal,68 : 145-47.
    12. Rahman, M. S., Islam, M. S., Rahman, M. T., Parvez, N. H., and Rahman, M.M. (2010). Morphometric analysis of vesicular glands of indigenous bull.International Journal of Sustainable Crop Production,5(1):11-4.
    13. Roy, K. S., Pawar, H. S., and Saigal, R. P. (1985). Histomorphological, histochemical and histoenzymological studies on prostate gland of ram (Ovisaries). Indian Journal of Animal Sciences, 55(12): 983-86.
    14. Roy, K. S., Pawar, H. S., and Saigal, R. P. (1987). Observations on seminal vesicle of ram (Ovisaries) a histochemical study. Indian Veterinary Journal, 64: 207-9.
    15. Scala, G. and Maruccio, L. (2012). Nitric oxide (NO) expression during annual reproductive activity in buffalo epididymis: A histochemical and immunocytochemical study. Theriogenology, 78 : 49–56
    16. Selvinjose, S., Basha, S. H., Balasubramanian, and Ramesh, G.(2009). Histochemical studies on the seminal vesicle in Madras red sheep (Ovisaries). Indian Journal of Veterinary Anatomy, 21(2): 5-8.
    17. Sudhakar, L. S. (1982). Histological and histochemical changes in the male accessory genital glands of Murrah buffalo during postnatal development. Ph.D. Thesis. Haryana Agricultural University, Hisar, India.
    18. Suri, S., Sudhakar, L. S., and Bhardwaj, R.L .(2012). Seasonal variation in the histomorphology and histochemistry of the bulbourethral gland of Gaddi goat and gaddi Sheep.Indian Journal of Veterinary Anatomy, 24 (1): 1-4.
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