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Research Article

volume 53 issue 10 (october 2019) : 1340-1343,   Doi: 10.18805/ijar.B-3655

Light and Electron microscopic studies on the sperm host glands in Punjab white quail 

S
S.V. Sukhadeve
N
Neelam Bansal
D
Devendra Pathak
1<div style="text-align: justify;">Department of Veterinary Anatomy, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.</div>
Cite article:- Sukhadeve S.V., Bansal Neelam, Pathak Devendra (2018). Light and Electron microscopic studies on the sperm host glands in Punjab white quail. Indian Journal of Animal Research. 53(10): 1340-1343. doi: 10.18805/ijar.B-3655.

ABSTRACT

The present work was conducted on oviduct of 42 Punjab white quails to observe the histomorphochemical and ultrastructural details of sperm host glands. The tissue samples were collected from the uterovaginal and infundiomagnal junctions of the oviduct in 10% NBF and 2.5% glutaralehyde solutions and processed for histological and electronmicroscopic studies. Fresh tissue samples were collected for cryostat sectioning to demonstrate lipids. It was observed that the UVJ was lined by pseudostratified columnar epithelium with ciliated, non ciliated cells, goblet cells and basal cells. The sperm host glands extended into lamina propria of UVJ as oval, rounded and straight tubules. The proximal part of glandular neck was lined by pseudostratified columnar ciliated epithelium and distal part with non ciliated columnar cells. The ciliated cells showed cilia and microvilli on the apical surface in the neck region, whereas the non ciliated cells present in the distal part of SHG had oval or elongated nuclei in their basal part and were studded with some of the microvilli in their luminal surface. These glands were surrounded by some of the smooth muscle cells and nerve fibers. Histochemical studies revealed a moderate to strong reaction of acid and neutral mucopolysaccharides and lipids whereas reaction for basic proteins was weak in the supranuclear part of SHG. 

REFERENCES

  1. Bakst, M. R. (1993). Oviductal sperm storage in poultry: A review. Reproduction, Fertility and development 5: 595-99.
  2. Bakst, M. R. and Akuffo, V. (2007). Alkaline phosphatase reactivity in the vagina and uterovaginal junction sperm-storage tubules of turkeys in egg production: implication for sperm storage. British Poultry Science 48: 515-18.
  3. Bakst, M. R. and Bird, D. M. (1987). Localization of oviductal sperm storage tubules in the American Kestrel (Falco sparverius). The Auk 104:321-24.
  4. Bansal, N., Uppal, V., Pathak, D. and Brah, G. S. (2010). Histomorphometrical and histochemical studies on the oviduct of Punjab White quails. Indian Journal of Poultry Science 45: 88-92.
  5. Bansal, N., Uppal, V., Sharma, A. and Brah, G. S. (2013). Histomorphometrical studies on the sperm host gland in quail. Indian Journal of Poultry Science 48: 406-09.
  6. Bezuidenhout, A. J., Soley, J. T., Groenewald, H. B. and Burger, W. P. (1995). Sperm-storage tubules in the vagina of the ostrich (Struthio camelus). Onderstepoort Journal of Veterinary Research. 62(3):193-9.
  7. Burke, W. H., Ogasawara, F. X. and Fuqua, C. L. (1972). A study of the ultrastructure of the uterovaginal sperm storage glands of the hen, Gallus domesticus in relation to a mechanism for the release of spermatozoa. Journal of Reproduction and fertility 29: 29-36.
  8. Das, S. K. ( 2003). Evidence for the innervation of sperm host glands of native chicken ‘s (Gallus domesticus) oviduct. International Journal of Poultry Science 2(4): 259-60.
  9. Deka, A., Sarma, K., Sarma, S., Goswami, J., Mahanta, J.D., Talukdar, M., Rajkhowa, J. and Perumal, P. (2018). Anatomical studies on the sperm storage organ of Pati and Chara-Chemballi Ducks. Indian Journal of Animal Research 52(4): 543-546.
  10. Frieâ, A. E., Sinowatz, F. and Wrobel, K. H. (1978). The uterovaginal sperm host glands of the quail (Coturnix coturnix japonica). Cell and Tissue Research 191: 101-14.
  11. Fuji, S. (1963). Histological and histochemical studies on the oviduct of the domestic fowl with special reference to the region of uterovaginal juncture. Archive of Histology 23: 447-559.
  12. Gilbert, A. B., Reynolds, M. E. and Lorenz, F. W. (1968). Distribution of spermatozoa in the oviduct and fertility in domestic birds. Journal of Reproduction and Fertility 16: 433-44.
  13. Ibrahim, I.A., Ahmed, A. K., Doaa, M. and Sara, M. M. (2015). Gross and microanatomical studies on the uterus of Japanese Quail (Coturnix japonica) during the post-hatching period with Special Emphasis on Sperm host gland. Journal of Veterinary Science and Technology 6: 272. doi:10.4172/2157-7579.1000272.
  14. King, L. M., Brillard, J. P., Bakst, M. R. and Donoghue, A. M. (1999). Isolation of sperm storage tubules from the uterovaginal junction mucosa of the turkey. Poultry Science 78: 1044-47.
  15. Koyanagi, F. and Nishiyama, H. (1981). Disintegration of spermatozoa in the infundibular sperm host glands of the fowl. Cell and Tissue Research 214: 81-87. 
  16. Luna, L. G. (1968). Manual of Histological Staining Methods of Armed Forces Institute of Pathology. 3rd edn. pp 38-196. Mc Graw Hill Book Company, New York, USA.
  17. Mansori, F. S. (2004). Histological study on the utero-vaginal junction on the laying hens in ross breed. In International Society for Animal Hygiene, Animal production in Europe, the way forward in a changing world pp: 465-66.
  18. Mehta, S. and Guha, K. (2012). Comparative histological study on the oviduct of developing and laying hens (Gallus domesticus). Indian Journal of Veterinary Anatomy 24: 92-94. 
  19. Moraes, C., Baraldi-Artoni, S. M., Pacheco, M. R., Oliveira, D., Amoroso, L. and Sagula, A. L. (2009). Histology and morphometry of the glands of the infundibulum-magnum and uterus-vagina junctions of domestic quail. Rural Science 39: 412-19.
  20. Rao, T. S. C. and Vijayragavan, C. (2000). Microanatomical studies on the transitional and host glands of the domestic duck (Anas boschas domesticus). Indian Journal of Veterinary Anatomy 1(12): 81-85. 
  21. Schuppin, G. T., Van Krey, H. P., Denbow, D. M., Bakst, M. R. and Meyer, G. B. (1984). Ultrastructural analyses of uterovaginal sperm storage glands in fertile and infertile turkey breeder hens. Poultry Science 63: 1872-82.
  22. Van Drimmelen, G. C. (1946). Spermnests in the oviduct of the domestic hen. Journal of South African Veterinary and Medical Association 17: 42-52.
  23. Van Krey, H. P., Ogasawara, F. X. and Pangborn, J. (1967). Light and electron microscopic studies of possible sperm gland emptying mechanism. Poultry Science 46: 69-78.
  24. Vijayakumar, K., Paramasivan, S. and Madhu, N. (2016). Microanatomy on oviduct of Laying and Non-Laying Emu Birds. (Dromaius Novaehollandiae). International Journal of Agricultural Science and Research 6: 89-96. 
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    Research Article

    volume 53 issue 10 (october 2019) : 1340-1343,   Doi: 10.18805/ijar.B-3655

    Light and Electron microscopic studies on the sperm host glands in Punjab white quail 

    S
    S.V. Sukhadeve
    N
    Neelam Bansal
    D
    Devendra Pathak
    1<div style="text-align: justify;">Department of Veterinary Anatomy, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.</div>
    Cite article:- Sukhadeve S.V., Bansal Neelam, Pathak Devendra (2018). Light and Electron microscopic studies on the sperm host glands in Punjab white quail. Indian Journal of Animal Research. 53(10): 1340-1343. doi: 10.18805/ijar.B-3655.

    ABSTRACT

    The present work was conducted on oviduct of 42 Punjab white quails to observe the histomorphochemical and ultrastructural details of sperm host glands. The tissue samples were collected from the uterovaginal and infundiomagnal junctions of the oviduct in 10% NBF and 2.5% glutaralehyde solutions and processed for histological and electronmicroscopic studies. Fresh tissue samples were collected for cryostat sectioning to demonstrate lipids. It was observed that the UVJ was lined by pseudostratified columnar epithelium with ciliated, non ciliated cells, goblet cells and basal cells. The sperm host glands extended into lamina propria of UVJ as oval, rounded and straight tubules. The proximal part of glandular neck was lined by pseudostratified columnar ciliated epithelium and distal part with non ciliated columnar cells. The ciliated cells showed cilia and microvilli on the apical surface in the neck region, whereas the non ciliated cells present in the distal part of SHG had oval or elongated nuclei in their basal part and were studded with some of the microvilli in their luminal surface. These glands were surrounded by some of the smooth muscle cells and nerve fibers. Histochemical studies revealed a moderate to strong reaction of acid and neutral mucopolysaccharides and lipids whereas reaction for basic proteins was weak in the supranuclear part of SHG. 

    REFERENCES

    1. Bakst, M. R. (1993). Oviductal sperm storage in poultry: A review. Reproduction, Fertility and development 5: 595-99.
    2. Bakst, M. R. and Akuffo, V. (2007). Alkaline phosphatase reactivity in the vagina and uterovaginal junction sperm-storage tubules of turkeys in egg production: implication for sperm storage. British Poultry Science 48: 515-18.
    3. Bakst, M. R. and Bird, D. M. (1987). Localization of oviductal sperm storage tubules in the American Kestrel (Falco sparverius). The Auk 104:321-24.
    4. Bansal, N., Uppal, V., Pathak, D. and Brah, G. S. (2010). Histomorphometrical and histochemical studies on the oviduct of Punjab White quails. Indian Journal of Poultry Science 45: 88-92.
    5. Bansal, N., Uppal, V., Sharma, A. and Brah, G. S. (2013). Histomorphometrical studies on the sperm host gland in quail. Indian Journal of Poultry Science 48: 406-09.
    6. Bezuidenhout, A. J., Soley, J. T., Groenewald, H. B. and Burger, W. P. (1995). Sperm-storage tubules in the vagina of the ostrich (Struthio camelus). Onderstepoort Journal of Veterinary Research. 62(3):193-9.
    7. Burke, W. H., Ogasawara, F. X. and Fuqua, C. L. (1972). A study of the ultrastructure of the uterovaginal sperm storage glands of the hen, Gallus domesticus in relation to a mechanism for the release of spermatozoa. Journal of Reproduction and fertility 29: 29-36.
    8. Das, S. K. ( 2003). Evidence for the innervation of sperm host glands of native chicken ‘s (Gallus domesticus) oviduct. International Journal of Poultry Science 2(4): 259-60.
    9. Deka, A., Sarma, K., Sarma, S., Goswami, J., Mahanta, J.D., Talukdar, M., Rajkhowa, J. and Perumal, P. (2018). Anatomical studies on the sperm storage organ of Pati and Chara-Chemballi Ducks. Indian Journal of Animal Research 52(4): 543-546.
    10. Frieâ, A. E., Sinowatz, F. and Wrobel, K. H. (1978). The uterovaginal sperm host glands of the quail (Coturnix coturnix japonica). Cell and Tissue Research 191: 101-14.
    11. Fuji, S. (1963). Histological and histochemical studies on the oviduct of the domestic fowl with special reference to the region of uterovaginal juncture. Archive of Histology 23: 447-559.
    12. Gilbert, A. B., Reynolds, M. E. and Lorenz, F. W. (1968). Distribution of spermatozoa in the oviduct and fertility in domestic birds. Journal of Reproduction and Fertility 16: 433-44.
    13. Ibrahim, I.A., Ahmed, A. K., Doaa, M. and Sara, M. M. (2015). Gross and microanatomical studies on the uterus of Japanese Quail (Coturnix japonica) during the post-hatching period with Special Emphasis on Sperm host gland. Journal of Veterinary Science and Technology 6: 272. doi:10.4172/2157-7579.1000272.
    14. King, L. M., Brillard, J. P., Bakst, M. R. and Donoghue, A. M. (1999). Isolation of sperm storage tubules from the uterovaginal junction mucosa of the turkey. Poultry Science 78: 1044-47.
    15. Koyanagi, F. and Nishiyama, H. (1981). Disintegration of spermatozoa in the infundibular sperm host glands of the fowl. Cell and Tissue Research 214: 81-87. 
    16. Luna, L. G. (1968). Manual of Histological Staining Methods of Armed Forces Institute of Pathology. 3rd edn. pp 38-196. Mc Graw Hill Book Company, New York, USA.
    17. Mansori, F. S. (2004). Histological study on the utero-vaginal junction on the laying hens in ross breed. In International Society for Animal Hygiene, Animal production in Europe, the way forward in a changing world pp: 465-66.
    18. Mehta, S. and Guha, K. (2012). Comparative histological study on the oviduct of developing and laying hens (Gallus domesticus). Indian Journal of Veterinary Anatomy 24: 92-94. 
    19. Moraes, C., Baraldi-Artoni, S. M., Pacheco, M. R., Oliveira, D., Amoroso, L. and Sagula, A. L. (2009). Histology and morphometry of the glands of the infundibulum-magnum and uterus-vagina junctions of domestic quail. Rural Science 39: 412-19.
    20. Rao, T. S. C. and Vijayragavan, C. (2000). Microanatomical studies on the transitional and host glands of the domestic duck (Anas boschas domesticus). Indian Journal of Veterinary Anatomy 1(12): 81-85. 
    21. Schuppin, G. T., Van Krey, H. P., Denbow, D. M., Bakst, M. R. and Meyer, G. B. (1984). Ultrastructural analyses of uterovaginal sperm storage glands in fertile and infertile turkey breeder hens. Poultry Science 63: 1872-82.
    22. Van Drimmelen, G. C. (1946). Spermnests in the oviduct of the domestic hen. Journal of South African Veterinary and Medical Association 17: 42-52.
    23. Van Krey, H. P., Ogasawara, F. X. and Pangborn, J. (1967). Light and electron microscopic studies of possible sperm gland emptying mechanism. Poultry Science 46: 69-78.
    24. Vijayakumar, K., Paramasivan, S. and Madhu, N. (2016). Microanatomy on oviduct of Laying and Non-Laying Emu Birds. (Dromaius Novaehollandiae). International Journal of Agricultural Science and Research 6: 89-96. 
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