Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

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Indian Journal of Animal Research, volume 53 issue 10 (october 2019) : 1335-1339

Isolation and characterization of neural stem cells from ovine foetal cerebral cortex

S. Lija, S. Eswari, M. Monisha, K. Vijayarani
1<div style="text-align: justify;">Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, Tamil Nadu, India.</div>
Cite article:- Lija S., Eswari S., Monisha M., Vijayarani K. (2018). Isolation and characterization of neural stem cells from ovine foetal cerebral cortex. Indian Journal of Animal Research. 53(10): 1335-1339. doi: 10.18805/ijar.B-3647.
Neural stem cells are a potential source in cell based therapy for neurodegenerative diseases and traumatic brain injuries. The aim of this study is to isolate the ovine (Ovis aries) fetal neural stem cells and to analyze their neurogenic characteristics in vitro. Cerebral cortex pieces harvested from ovine fetal brain tissue were triturated for dispersal into single cells, cultured in neuronal stem cells culture medium and expanded in vitro in adherent culture. The morphological changes and the expression of neuron-specific gene markers and proteins were evaluated. These isolated NSCs were plastic adherent, self-renewing cells maintained the capacity to differentiate into brain specific cell types and they were found positive for neuronal stem cell markers, Nestin and â III tubulin by Reverse Transcription-PCR. Immunocytochemistry of the isolated NSCs also showed positive for Nestin, an established marker for neural and glial precursors as well as MSC marker CD90 and negative for  hematopoietic CD34 markers. 
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