Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 53 issue 9 (september 2019) : 1234-1238

Prevalence and molecular characterisation of Cryptosporidium spp. in goat kids 
 

Pooja Dixit, M.L.V. Rao, A.K. Dixit, Rohita Gupta, P.C. Shukla
1Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur - 482 001, India.
Cite article:- Dixit Pooja, Rao M.L.V., Dixit A.K., Gupta Rohita, Shukla P.C. (2018). Prevalence and molecular characterisation of Cryptosporidium spp. in goat kids. Indian Journal of Animal Research. 53(9): 1234-1238. doi: 10.18805/ijar.B-3642.

ABSTRACT

Prevalence and molecular characterisation of Cryptosporidium species was done in kids belonging to organised and non-organised goat farms at Jabalpur. The overall prevalence of Cryptosporidium was 14.63%. The prevalence was non-significantly higher in male kids (16.16%) as compared to that of female kids (13.21%). Age wise prevalence was higher in kids up to one month age (16.13%) than that of kids upto 3 months age (13.99%). No significant difference was found in prevalence among different breeds and in kids kept in farm or field conditions. The prevalence was non-significantly higher in non-diarrhoeic kids than diarrhoeic kids. Most of the infections were of one score (76.6%). Molecular characterisation by PCR-RFLP of 18S SSU rRNA gene revealed presence of Cryptosporidium parvum species in positive faecal samples.
 

REFERENCES

  1. Agrawal, R. (2013). Epidemiological and therapeutic studies in calf cryptosporidiosis. Ph.D. thesis (Veterinary Medicine), Nanaji Deshmukh Veterinary Science University, Jabalpur.
  2. Ahamed, I., Yadav, A., Katoch, R., Godara, R. (2014). Longitudinal study of cryptosporidiosis in lambs from birth to one year of age. Indian Veterinary Journal, 91(10): 63 – 65.
  3. Arrowood, M. J. and Sterling, C.R. (1987). Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic percoll gradients. The Journal of Parasitology, 73(2): 314-319.
  4. Bejan, A., Mircean, V., Radu, C., Smaro, S., Cozma, V. (2009). Epidemiology of Cryptosporidium spp. infection in goat kids in the central and the northwest part of Romania. Revista Scientia Parasitologica, 10: 32-36.
  5. Bhat, S. A., Juyal, P. D., Singh, N.K., and Singla, L.D. (2013). Coprological investigation on neonatal bovine cryptosporidiosis in Ludhiana, Punjab. Journal of Parasitic Diseases, 37(1):114-117.
  6. Bhat, S.A., Juyal, P.D. and Singla, L.D. (2012). Prevalence of cryptosporidiosis in neonatal buffalo calves in Ludhiana district of Punjab, India. Asian Journal of Animal and Veterinary Advances, 7(6): 512-520.
  7. Brar, A.P.S., Sood, N.K., Kaur, P., Singla, L.D., Sandhu, B.S., Gupta, K., Narang, D., Singh, C.K. and Chandra, M. (2017). Periurban outbreaks of bovine calf scours in Northern India caused by Cryptosporidium in association with other enteropathogens. Epidemiology and Infection, 145 (13): 2717-2726
  8. Brar, A.P.S., Sood, N.K., Singla, L.D., Kaur, P., Gupta, K. and Sandhu, B.S. (2016). Validation of Romanowsky staining as a novel screening for the detection of faecal cryptosporidial oocysts. Journal of Parasitic Diseases, 41 (1): 260-262.
  9. Castro-Hermida, J.A., González-Losada, Y.A. and Ares-Mazás, E. (2002). Prevalence of and risk factors involved in the spread of neonatal bovine cryptosporidiosis in Galicia (NW Spain). Veterinary Parasitology, 106(1):1-10.
  10. Causape, A.C., Quýìlez, J., Sánchez-Acedo, C., del Cacho, E. and López-Bernad, F. (2002). Prevalence and analysis of potential risk factors for Cryptosporidium parvum infection in lambs in Zaragoza (Northeastern Spain). Veterinary Parasitology, 104(4):287-    298.
  11. Current, W.L., Reese, N.C., Ernst, J.V., Bailey, W.S., Heyman, M.B. and Weinstein, W.M. (1983). Human cryptosporidiosis in immunocompetent and immunodeficient persons: Studies on outbreak and experimental transmission. New England Journal of Medicine, 308: 1252–1258.
  12. Feng, Y., Ortega, Y., He, G., Das, P., Xu, M., Zhang, X., Fayer, R., Gatei, W., Cama, V., Xiao, L. (2007). Wide geographic distribution of Cryptosporidium bovis and the deer-like genotype in bovines. Veterinary Parasitology, 144: 1–9.
  13. Geurden, T., Thomas, P., Casaert, S., Vercruysse, J., Claerebout, E. (2008). Prevalence and molecular characterisation of Cryptosporidium and Giardia in lambs and goat kids in Belgium. Veterinary Parasitology, 155(1-2): 142-145.
  14. Henriksen, S.A., Pohlenz, J.F.L. (1981). Staining of cryptosporidia by a modified Ziehl-Neelsen technique. Acta Veterinaria Scandinavica, 22: 594-596.
  15. Inpankaew, T., Jiyipong, T., Wongpanit, K., Pinyopanuwat, N., Chimnoi, W., Kengradomkij, C., Xuan, X., Igarashi, I., Xiao, L., Jittapalapong, S. (2014). Molecular detection of Cryptosporidium spp. infections in water buffaloes from Northeast Thailand. Tropical Animal Health and Production, 46(2): 487-490.
  16. Jafari, R., Maghsood, A.H. and Fallah, M. (2012). Prevalence of Cryptosporidium infection among livestock and humans in contact with livestock in hamadan district, Iran, 2012. Journal of Research in Health Sciences, 13(1): 86-89.
  17. Jiang, J., Alderisio, K.A., Singh, A., Xiao, L. (2005). Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors. Applied and Environmental Microbiology, 71(3): 1135-1141.
  18. Khezri, M. and Khezri, O. (2013). The prevalence of Cryptosporidium spp. in lambs and goat kids in Kurdistan, Iran. Veterinary World, 6(12): 974-977.
  19. Koinari, M., Lymbery, A.J., Ryan, U.M. (2014). Cryptosporidium species in sheep and goats from Papua New Guinea. Experimental Parasitology, 14: 134-137.
  20. Lorenz, I., Fagan, J., More, S.J. (2011). Calf health from birth to weaning. II. Management of diarrhoea in pre-weaned calves. Irish Veterinary Journal, 64(1): 1.
  21. Marreros, N., Frey, C.F., Willisch, C.S., Signer, C. and Ryser-Degiorgis, M.P. (2012). Coprological analyses on apparently healthy Alpine ibex (Capra ibex ibex) from two Swiss colonies. Veterinary Parasitology, 186(3): 382-389.
  22. Maurya, P.S., Rakesh, R.L., Pradeep, B., Kumar, S., Kundu, K., Garg, R., Ram, H., Kumar, A., Banerjee, P.S. (2013). Prevalence and risk factors associated with Cryptosporidium spp. infection in young domestic livestock in India. Tropical Animal Health and Production, 45: 941–946.
  23. Misic, Z.B., Katic-Radivojevic, S.P. and Kulisic, Z. (2006). Cryptosporidium infection in lambs and goat kids in Serbia. Acta Veterinaria,    56(1): 49-54.
  24. Noordeen, F., Rajapakse, R.P.V.J., Faizal, A.C.M., Horadagoda, N.U., Arulkanthan, A. (2000). Prevalence of Cryptosporidium infection in goats in selected locations in three agroclimatic zones of Sri Lanka. Veterinary Parasitology, 93: 95–101.
  25. Olson, M.E., Thorlakson, C.L., Deselliers, L., Morck, D.W., McAllister, T.A. (1997). Giardia and Cryptosporidium in Canadian farm animals. Veterinary Parasitology, 68(4): 375-381.
  26. Paul, S., Chandra, D., Ray, D.D., Tewari, A.K., Rao, J.R., Banerjee, P.S., Baidya, S., Raina, O.K. (2008). Prevalence and molecular characterization of bovine Cryptosporidium isolates in India. Veterinary Parasitology, 153(1-2): 143-146.
  27. Paul, S., Reddy, M.G.B., Sharma, D.K. (2013). Cryptosporidiosis in neonatal goat kids in North–Western India. Indian Veterinary Journal, 90: 142–143.
  28. Putignani, L., Menichella, D. (2010). Global distribution, public health and clinical impact of the protozoan pathogen Cryptosporidium.    Interdisciplinary Perspectives on Infectious Diseases, 2010: 39.
  29. Quilez, J., Sanchez-Acedo, C., Del Cacho, E., Clavel, A., Causape, A.C. (1996). Prevalence of Cryptosporidium and Giardia infections in cattle in Aragon (Northeastern Spain). Veterinary Parasitology, 66(3):139-146.
  30. Rajendran, P., Ajjampur, S.S.R., Chidambaram, D., Kattula, D., Rajan, D.P., Ward, H. and Kang, G. (2011). Investigation of potential zoonotic transmission of cryptosporidiosis in Southern India. The American Society of Tropical Medicine and Hygiene, 85: 657–659.
  31. Randhawa, S.S., Randhawa, S.S., Zahid, U.N., Singla, L.D. and Juyal, P.D. (2012). Drug combination therapy in control of cryptosporidiosis in Ludhiana district of Punjab. Journal of Parasitic Diseases,36: 269-272.
  32. Roy, S.S., Sarkar, S., Batabyal, S., Pramanik, A.K., Das, P. (2006). Observations on the epidemiology of bovine cryptosporidiosis in India. Veterinary Parasitology,141(3): 330-333.
  33. Sanz-Ceballos, L., Illescas Gómez, P., Sanz Sampelayo, M.R., Gil Extremera, F., Osorio, R. (2009). Prevalence of Cryptosporidium infection in goats maintained under semi-extensive feeding conditions in the southeast of Spain. Parasite, 16(4): 315-318.
  34. Singla, L.D., Gupta, M.P., Singh, H., Singh, S.T., Kaur, P. and Juyal, P.D. (2013). Antigen based diagnosis of Cryptosporidium parvum infection in faeces of cattle and buffalo calves. Indian Journal of Animal Science, 83(1): 37-39.
  35. Snedecor, G.W., Cochran, W.G. (1994). Statistical Methods (7th Edn.) Oxford and IBH Publishing Co., New Delhi, 350p.
  36. Wang, R., Li, G., Cui, B., Huang, J., Cui, Z., Zhang, S., Dong, H., Yue, D., Zhang, L., Ning, C., and Wang, M. (2014). Prevalence, molecular characterization and zoonotic potential of Cryptosporidium spp. in goats in Henan and Chongqing, China. Experimental Parasitology, 142:11-16.
  37. Xiao, L. (2010). Molecular epidemiology of cryptosporidiosis: an update. Experimental Parasitology, 124(1): 80-89.
  38. Xiao, L., Bern, C., Limor, J., Sulaiman, I., Roberts, J., Checkley, W., Cabrera, L., Gilman, R.H., Lal, A.A. (2001). Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru. Journal of Infectious Diseases, 183(3): 492-497.
  39. Xiao, L., Escalante, L., Yang, C., Sulaiman, I., Escalante, A.A., Monsali, R.J., Fayer, R. and Lal, A.A. (1999). Phylogenetic analysis of Cryptosporidium parasites based on the ssu rRNA gene locus. Applied and Environmental Microbiology, 65: 1578–1583. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)