Effect of probiotics mix supplementation on haemato-biochemical parameters and bacterial faecal shedding in Corriedale lambs fed paddy straw based complete feed

DOI: 10.18805/ijar.B-3622    | Article Id: B-3622 | Page : 1049-1053
Citation :- Effect of probiotics mix supplementation on haemato-biochemical parameters and bacterial faecal shedding in Corriedale lambs fed paddy straw based complete feed.Indian Journal Of Animal Research.2019.(53):1049-1053
G.G. Sheikh, Danish Masood, A.M. Ganai, S. Muzamil, Y. Afzal and H.A. Ahmad gull2217@gmail.com
Address : Division of Animal Nutrition, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shuhama, Alusteng, Srinagar-190 006, Jammu and Kashmir, India.
Submitted Date : 25-04-2018
Accepted Date : 12-09-2018

Abstract

Growth trial of 90 days was conducted on 12 male Corriedale lambs, divided in two groups of six lambs each, to study the effect of feeding of paddy straw based complete feed supplemented of probiotic mix (Saccharomyces cerevisiae 2×1010cfu/g + Lactobacillus acidophilus 6×109cfu/g) in equal ratio @ 3 % of DM, as per the in vitro studies, while complete feed without probiotics served as control. There was significantly (P<0.01)  higher haemoglobin concentration, RBC, WBC and monocyte count in animals fed probiotic mix than control, with no significant effect on PCV, lymphocyte, eosinophill and neutrophill count. Blood glucose, total serum protein, albumin, globulin, BUN and serum creatinine values were statistically higher in probiotic mix supplemented animals. Lambs fed with probiotics show significant (P<0.01) decrease in serum cholesterol, LDL-cholesterol and triglycerides with increment of HDL-cholesterol than animals fed unsupplemented diet, with no effect on enzyme activities of liver enzymes. The lambs fed on probiotic mix shows significant reduction in faecal shedding of E. coli and total coliform with firm pellets faecal consistency. Probiotic mix has great potential to beneficially affect the haemato-biochemical parameters and gut micro-flora and hence improve physiological health status of animals.

Keywords

Bacterial faecal shedding Complete feed Haemato-biochemical Probiotics mix.

References

  1. Abu El-Ella A.A. and Kommonna, O.F. (2013). Reproductive performance and blood constituents of Damascus goats as affected by yeast culture supplementation. Egyptian Journal of Sheep and Goat Sciences, 8: 171-187. 
  2. Agarwal N., Kamra, D. N., Chaudhary, L. C., Sahoo, A. and Pathak, N.N. (2002). Microbial status and rumen enzyme profile of cross bred calves fed on different microbial feed additives. Letters in Applied Microbiol,34: 329-336. 
  3. Bedy, I. F. (2014). The effect of diet fermentation with locally probiotics on some productive, microbiological, immunological and histological properties of broiler chicks.Doctoral thesis, College of Agriculture at the University of Baghdad, Baghdad.
  4. Bruno, R. G. S., Rutigliano, H. M., Cerri, R. L., Robinson, P. H. and Santos, J. E. P. (2009). Effect of feeding Saccharomyces Cerevisiae on performance of dairy cows during summer heat stress. Animal Feed Science Technology. 150:175-186.
  5. Chapman, P. and Siddons, C. (1994). A comparison of immunomagnetic and direct culture for the isolation of verocytotoxin producing Escherichia coli O157:H7 from bovine feces. Journal of Medical Microbiology. 40:424-427.
  6. Deka, R.S. (2009). Effect of probiotic biobloom as a growth promotor in kids. Indian Veterinary Journal 86: 1192-1193.
  7. Doto, S.P., Liu, J. and Wang, W. (2011). Effect of yeast culture and direct fed microbes on the growth performance and rumen fermentation of weaner lambs. Journal of Animal Science and Biotechnology 2:208-216.
  8. El-Marakby, K. M. A. (2003). Biological treatment of poor quality roughage and its effect on productive performance of ruminants. M.Sc. thesis. Fac. Agric., Zagazing University, Egypt, pp. 42-47.
  9. EL-Moghazy, M., Husain, M., El-Fadaly, Tag EL-Din, Hamada Areda, A. (2015). Effect of sheep diets containing microbiological treated rice straw on blood parameters and nitrogen balance. Journal of Microbiology Research. 5 (2): 46-56
  10. FAOD WHO. (2001). Evaluation of health and nutritional properties of powder milk andlive lactic acid bacteria. Food andAgriculture Organization of the United Nations and World Health Organization Expert Consultation Report, Cordoba, Argentina, pp. 1-34
  11. Fuller, R. (1989). A review: Probiotics in man and animals. Journal of Applied Bacteriology. 66: 365-378. 
  12. Ghazanfar, S., Anjum, M. I., Azim, A. and Ahmed, I. (2015). Effects of dietary supplementation of yeast (Saccharomyces cerevisiae) culture on growth performance, blood parameters, nutrient digestibility and fecal flora of dairy heifers. Journal of Animal & Plant Science. 25(1): 53-59.
  13. Hillal, H., El-Sayaad, G. and Abdella, M. (2011). Effect of growth promoters (probiotics) supplementation on performance, rumen activity and some blood constituents in growing lambs, Archiv Tierzucht. 54:607-617.
  14. Hussein, A. F. (2014). Effect of biological additives on growth indices and physiological responses of weaned Najdi ram lambs. Journal of Experimental Biology and Agricultural Science. 2(6).
  15. ICAR. (2013). Indian council of agricultural research. Nutrient requirements of cattle. ICAR New Delhi, India.
  16. Issakowicz, J., Bueno, M. S., Sampaio, A. C. K. and Duarte, K. M. R. (2013). Effect of concentrate level and live yeast (Saccharomyces cerevisiae) supplementation on Texel lamb performance and carcass characteristics. Livestock Science.155:44-52.
  17. Kalavathy, R., Abdullah, N., Jalaludin, S., Wong, C.M.V.L and Ho. Y. W. (2006). Effects of Lactobacillus feed supplementation on cholesterol, fat content and fatty acid composition of the liver, muscle and carcass of broiler chickens. Animal Research. 55: 77-82. 
  18. Kaneko, J. J., Harvey, J. W. and Bruss, M. Z. (1997). In: Clinical Biochemistry of Domestic Animals. 5th Edition.Academic Press, USA.
  19. Kawakami, S. I., Yamada, T., Nakanishi, N. and Cai, Y. (2010). Feeding of lactic acid bacteria and yeast on growth and diarrhea of Holstein calves. Journal of Animal and Veterinary Advances. 9(7):1112-1114.
  20. Khaled, N.F. and Baraka, T.A. (2011). Influence of direct-fed microbials on productive performance, selected rumen and blood constituents in barky finishing lambs. Journal of American Science.7:(9).
  21. Lema, M., Williams, L. and Rao, D. R. (2001). Reduction of fecal shedding of enterohemorrhagic Escherichia coli O157:H7 in lambs by feeding microbial feed supplement. Small Ruminant Research. 39: 31-39.
  22. Lim, H. J., Kim, S. and Lee, W. (2004). Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use. Journal of Veterinary Science. 5: 391-395. 
  23. Mahmmod, Z. A., Abdulrazaq, H. S., Salem, A. S. and Sideq, R. M. (2014). Effects of supplementation probiotic and dried yogurt powder on growth performance, carcass characteristics, intestinal micro flora and immunity of broiler chickens. Zanco Journal of Pure and Applied Sciences. 26: 35-42.
  24. Masek, T., Mikulee, Z., Valpotic, H., Kusce, L., Mikulec, N. and Antunac, N. (2008). The influence of live yeast cells (Saccharomyces cerevisiae) on the performance of grazing dairy sheep in late lactation. Veterinarski Arhiv.78(2): 95-104.
  25. Milewski, S. and Sobiech, P. (2009). Effect of dietary supplementation with Saccharomyces cerevisiae dried yeast on milk yield, blood biochemical and hematological indices in ewes. Bulletin of the Veterinary Institute in Pulawy.53: 753758. 
  26. Miyazaki,, Y., Kamiya, S., Hanawa, T. and Yokota, H. (2010). Effect of probiotic bacterial strains of Lactobacillus, Bifidobacterium and Enterococcus on enteroaggregative Escherichia coli. Journal of Infection and Chemotherapy. 16(1):10-8.
  27. Mousa, K. M., El-Malky, O. M., Komonna, O. F., Rashwan, S. E. (2012). Effect of some yeast and minerals on the productive and reproductive performance in ruminants. Journal of American Science. 8: 291-303. 
  28. Musa, H. H., Wu, S. L., Zhu, C. H., Seri, H. I., Zhu, G. Q. (2009). The potential benefits of probiotics in animal production and health. Journal of Animal and Veterinary Advances. 8: 313– 321. 
  29. Signorini, M. L., Sot, L. P., Zbrun, M. V., Sequeira, G. J., Rosmini, M. R. and Frizzo, L. S. (2012). Impact of probiotic administration on the health and fecal microbiota of young calves: a meta-analysis of randomized controlled trials of lactic acid bacteria. Research in Veterinary Science. 93: 250-258.
  30. Soren, N.M., Tripathi, M.K., Bhatt, R.S. and Karim, S.A. 2013. Effect of yeast supplementation on the growth performance of malpura lambs. Tropical Animal Health Production 45:547-554
  31. Stephens, T. P., Loneragan, G. H., Karunasena, E. and Brashears, M. M. (2007). Reduction of Escherichia coli O157 and Salmonella in feces and on hides of feedlot cattle using various doses of a direct-fed microbial. Journal of Food Protection. 70: 1346-    1349.
  32. Whitley, N.C., Cazac, D., Rude, B.J., Jackson-O’Brien, D. and Parveen, S. (2009). Use of a commercial probiotic supplement in meat goats. Journal of Animal Science 87:723-728. 

Global Footprints