Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 53 issue 8 (august 2019) : 1020-1024

Effect of antioxidant ascorbic acid on in vitro maturation of Caprine oocytes under normal and elevated temperatures

S.B. Khanday, J.A. Ahmed, N. Nashiruddullah, U. Sharma, D. Chakraborty
1Division of Veterinary Physiology and Biochemistry Faculty of Veterinary Science and Animal Husbandry. Sher-e-Kashmir University of Animal Sciences and Technology. R.S. Pura-181 102, Jammu & Kashmir, India
Cite article:- Khanday S.B., Ahmed J.A., Nashiruddullah N., Sharma U., Chakraborty D. (2018). Effect of antioxidant ascorbic acid on in vitro maturation of Caprine oocytes under normal and elevated temperatures. Indian Journal of Animal Research. 53(8): 1020-1024. doi: 10.18805/ijar.B-3616.

ABSTRACT

The aim of the present study was to assess the effect of ascorbic acid on in vitro maturation of caprine oocytes under normal and elevated temperatures. Goat ovaries were collected at slaughter and both A and B grade cumulus-oocyte-complexes (COCs) were aspirated out and were matured in vitro under normal (38.5°C) and elevated temperatures (41°C). On the basis of cumulus expansion and nuclear maturation, the maturation competencewere compared with and without ascorbic acid supplementation (100 µM). Heat stress significantly (P£ 0.01) reduced cumulus expansion, maturation rate and lowered metaphase stage II of nuclear maturation. Ascorbic acid improved developmental competence of oocytes during heat stress (41 °C) and ascorbic acid supplemented COCs demonstrated significantly (P£ 0.05) higher maturation rates when compared to non-supplemented groups.

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