Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 53 issue 8 (august 2019) : 1020-1024

Effect of antioxidant ascorbic acid on in vitro maturation of Caprine oocytes under normal and elevated temperatures

S.B. Khanday, J.A. Ahmed, N. Nashiruddullah, U. Sharma, D. Chakraborty
1Division of Veterinary Physiology and Biochemistry Faculty of Veterinary Science and Animal Husbandry. Sher-e-Kashmir University of Animal Sciences and Technology. R.S. Pura-181 102, Jammu & Kashmir, India
Cite article:- Khanday S.B., Ahmed J.A., Nashiruddullah N., Sharma U., Chakraborty D. (2018). Effect of antioxidant ascorbic acid on in vitro maturation of Caprine oocytes under normal and elevated temperatures. Indian Journal of Animal Research. 53(8): 1020-1024. doi: 10.18805/ijar.B-3616.
The aim of the present study was to assess the effect of ascorbic acid on in vitro maturation of caprine oocytes under normal and elevated temperatures. Goat ovaries were collected at slaughter and both A and B grade cumulus-oocyte-complexes (COCs) were aspirated out and were matured in vitro under normal (38.5°C) and elevated temperatures (41°C). On the basis of cumulus expansion and nuclear maturation, the maturation competencewere compared with and without ascorbic acid supplementation (100 µM). Heat stress significantly (P£ 0.01) reduced cumulus expansion, maturation rate and lowered metaphase stage II of nuclear maturation. Ascorbic acid improved developmental competence of oocytes during heat stress (41 °C) and ascorbic acid supplemented COCs demonstrated significantly (P£ 0.05) higher maturation rates when compared to non-supplemented groups.
  1. Ali, A.A.,;Bilodeau, J.F., Sirard, M.A. (2003). Antioxidant requirements for bovine oocytes ovaries during in vitro maturation, fertilization and development. Theriogenology, 59(3-4): 939-949.
  2. Castillo-Martin, M., Bonet, S.,Morato, R.S.B., Yeste, M.(2014b). Supplementing culture and vitrification warming media with L-    ascorbic acid enhances survival rates and redox status of IVP porcine blastocysts via induction of GPX1 and SOD1 expression. Cryobiology., 68(3): 451-458.
  3. Castillo-Martin, M., Yeste, M., Soler, A., Morato, R.S.B. (2014a). Comparative effects of adding â-mercaptoethanol or L-ascorbic acid to culture or vitrification warming media on IVF porcine embryos. Reproduction, Fertility, and Development, 26(6): 875-882.
  4. Das, P.C., Das, K.P., Bagchi, K., Dey, C.D. (1993). Evaluation of tissue ascorbic acid status in different hormonal states of female rat. Life Science, 52: 1493-1498.
  5. Dorra, T.M., Adbel-Khalez, A.E., Raya, A.H., Fawzy, S.A., El-Raate, S.M.S.I.T. (2012). Effects of ascorbic acid and glutathione on in vitro maturation of New Zealand white and Baladi Black rabbit oocytes. Egyptian Journal Basicand Applied Physiology,. pp.: 1-15.
  6. Ealy, A.D., Drost, M., Hansen, P.J. (1993). Developmental changes in embryonic resistance to adverse effects of maternal heat stress in cows. Journal of Dairy Sciences., 76: 2899-2905.
  7. Elsayed, M.A., Taha, N.A., Hammam, A.M.M., Sawiress, F.A.R. (2015). Effect of antioxidant supplementation on in vitro maturation of Camelus dromedaries oocytes. Nature and Science, 13(2): 17-24.
  8. Eppig, J.J., Hosoe, M., O’Brien, M.J., Pendola, F.M., Requena, A., Watanabe, S. (2000). Conditions that affect acquisition of developmental competence by mouse oocytes in vitro: FSH, insulin, glucose and ascorbic acid. Molecular and Cellular Endocrinology., 163: 109-116. 
  9. Hammami, S. (2014). In vitro embryo production from prepubertal goat oocytes in different culture media. Doctoral Thesis submitted to Universitat Autonoma de Barcelona, Spain.
  10. Hammami, S., Morató, R., Romaguera, R., Roura, M., Catalá, M.G., Paramio, M.T., Mogas, T., Izquierdo, D. (2013). Developmental competence and embryo quality of small oocytes from pre-pubertal goats cultured in IVM medium supplemented with low level of hormones, insulin-transferrin-selenium and ascorbic acid. Reproduction in Domestic Animals, 48(2): 339-44. 
  11. Hossein, M.S., Hashem, M.A., Jeong, Y.W., Lee, M.S., Kim, S., Kim, J.H., Koo, O.J., et al. (2007). Temporal effects of A-Tocopherol and L-Ascorbic acid on in vitro fertilized porcine embryo development. Animal Reproduction Science, 100(1-2): 107-117.
  12. Jainudeen, M.R., Wahid, H., Hafez, E.S.E. (2000). Ovulation, induction, embryo production and transfer assisted reproductive technology. In: Reproduction in Farm Aanimals, [Edited by:Hafez, B and Hafez, E.S.E.,] 7thedition, Lea and Febiger, Philadelphia, Ppp: 418.
  13. Jyotiranjan, T., Mohapatra, S., Mishra, C., Dalai, N., Kundu, A.K. (2017). Heat tolerance in goat- A genetic update. The Pharma Innovation Journal, 6(9): 237-245.
  14. Kere, M., Siriboon, C., Lo, N.W., Ju, J.C. (2012). Ascorbic acid improves the developmental competence of porcine oocytes after parthenogenetic activation and somatic cell nuclear transplantation. European Journal of Applied Sciences, 4(4): 182-190.
  15. Kosower, N.S., Kosower, E.M. (1973). Protection of membranes by glutathione: In Glutathione: Proceedings of 6th Conference of the German Society of Biological Chemistry, [edited by Flohe, L., Benoher, H.CH., Sies, H., Waller, H.D. and Wendel, A]. Academic press, New York, pp. 216-226.
  16. Lutwak-Mann, C. (1954). Note on the chemical composition of bovine follicular fluid. A.R.C Unit of Animal Reproduction, School of Agriculture, University of Cambridge, 44: 477-480.
  17. Miclea, I; Zãhan. M; Rusu, A; Ghiuru, F and Miclea, V. (2008). The effect of several ascorbic acid concentrations on swine oocyte maturation and embryo culture. Universitatea de ªtiinþe Agricoleºi Medicinã Veterinarã Iaºi Lucrãri ªtiinþifice, 52: 35-40.
  18. Murray, A.A., Molinek, M.D., Baker, S.J., Kojima, F.N., Smith, M.F., Hillier, S.G., Spears, N. (2001). Role of ascorbic acid in promoting follicle integrity and survival in intact mouse ovarian foliclesfollicles in vitro. Reproduction, 121(1): 89-96.
  19. Nabenishi, H., Ohta, H., Nishimoto, T., Morita, T., Ashizawa, K., Tsuzuki, Y. (2012a). The effects of cysteine addition during in vitro maturation on the developmental competence, ROS, GSH and apoptosis level of bovine oocytes exposed to heat stress. Zygote, 20: 249-259.
  20. Nabenishi, H., Takagi, S.,Kamata, H., Nishimoto, T., Morita, T., Ashiwaza, K., Tsuzuki, Y. (2012b). The role of mitochondrial transition pores on bovine oocyte competence after heat stress, as determined by effects of cyclosporin A. Molecular Reproduction and Development,. 79: 31-40.
  21. Nadri, B., Zeinoaldini, S., Kohram, H. (2009). Ascorbic acid effects on in vitro maturation of mouse oocyte with or without cumulus cells. African Journal of Biotechnology, 8(20): 5627-5631.
  22. Padh, H. (1991). Vitamin C: Newer insights into its biochemical functions. Nutrition Reviews, 49: 65-70.
  23. Pernes, A., Miclea, I., Zahan, M., Miclea, V., Orlovschi, D., Codea, A.R. (2016). The influence of ascorbic acid on in vitro maturation of canine oocytes. Bulletin UASVM Animal Science and Biotechnologies, 73(2):230-234
  24. Robertshaw, D (2004). Temperature Regulation and Thermal Environment, Chapter 55 by DavidRobertshaw. In: Dukes’ Physiology of Domestic Animals, 12th edn., Ed: Reece W.O, Ed. Cornell University.
  25. Rose, R.C. and Bode, A.M. (1993). Biology of free radical scavengers: an evaluation of ascorbate. The FASEB Journal, 7(12): 1135-1142.
  26. Roth, Z. and Hansen, P.J. (2004b). Involvement of apoptosis in disruption of developmental competence of bovine oocytes by heat shock during maturation. Biology of Reproduction, 71: 1898-1906.
  27. Tatemoto, H., Sakurai, N., Muto, N. (2000). Protection of porcine oocytes against apoptotic cell death caused by oxidative stress during in vitro maturation: Role of cumulus cells. Biology of Reproduction, 63: 805-810.
  28. Tilly, J.L. and Tilly, K.I. (1995). Inhibitors of oxidative stress mimic the ability of follicle-stimulating hormone to suppress apoptosis in cultured rat ovarian follicles. Endocrinology, 136(1): 242-252. 

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