Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 53 issue 7 (july 2019) : 959-962

Detection of Mycoplasma gallinaceum by PCR amplification of the 16S rRNA gene from respiratory disease in village chickens

P. Ponnusamy, T. Lurthu Reetha, B.S.M. Ronald, B. Puvarajan, R. Manicakm
1Department of Veterinary Microbiology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Orathanadu - 614 625, Tamil Nadu, India.
Cite article:- Ponnusamy P., Reetha Lurthu T., Ronald B.S.M., Puvarajan B., Manicakm R. (2018). Detection of Mycoplasma gallinaceum by PCR amplification of the 16S rRNA gene from respiratory disease in village chickens. Indian Journal of Animal Research. 53(7): 959-962. doi: 10.18805/ijar.B-3604.

ABSTRACT

In the present study, Mycoplasma gallinaceum was detected by PCR amplification of 16S rRNA gene from chronic respiratory disease in village chickens of Cauvery delta region of Tamil Nadu. Necropsy was performed to find out the etiological agent in desi birds mortality. At necropsy, airsacculitis with caseous exudate were found in the thoracic and abdominal cavity. Caseous material from airsacs was collected aseptically from dead birds for detection of Mycoplasma species. DNA was extracted from caseous material by using tissue DNA extraction kit. PCR was carried out using primers to amplify 16S rRNA gene belonging to Mycoplasma species. The amplified product yielded approximately 700-bp length (703 to 713 bp) of the 16S rRNA gene specific for Mycoplasma species. Further, it was subjected to sequence analysis and confirmed as Mycoplasma gallinaceum by NCBI blast analysis.  In the present communication, detection of M. gallinaceum by PCR amplification of 16S rRNA gene provides a powerful tool for rapid diagnosis. 

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