Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 53 issue 7 (july 2019) : 938-943

Phenotypic and molecular characterization of extended spectrum b-lactamase, ampc b-lactamase and metallo b-lactamase producing Klebsiella spp. from farm animals in India

Rituparna Tewari, Susweta Mitra, Nimita Venugopal, Sangita Das, Feroze Ganaie, Arnab Sen, Rajeswari Shome, Habibur Rahman, Bibek Ranjan Shome
1ICAR-National Institute of Veterinary Epidemiology and Disease, Informatics, Bengaluru-560 064, Karnataka, India.
Cite article:- Tewari Rituparna, Mitra Susweta, Venugopal Nimita, Das Sangita, Ganaie Feroze, Sen Arnab, Shome Rajeswari, Rahman Habibur, Shome Ranjan Bibek (2018). Phenotypic and molecular characterization of extended spectrum b-lactamase, ampc b-lactamase and metallo b-lactamase producing Klebsiella spp. from farm animals in India. Indian Journal of Animal Research. 53(7): 938-943. doi: 10.18805/ijar.B-3599.

ABSTRACT

Animal populace has attained less attention in antimicrobial resistance research than human sector resulting in limited information available on animal origin isolates. The study aimed to investigate the occurrence of ESBL, AmpC and MBL genes, plasmids and integrons in Klebsiella spp. Fecal samples were collected from healthy livestock (cattle, pig, sheep and goat) and poultry between  2012-2015. Preliminary identification of isolates was done by conventional phenotypic methods and confirmed by genotypic methods. Antimicrobial susceptibility testing was performed by disk diffusion method. Molecular characterization by PCR was conducted for 17 antimicrobial resistance genes, 3 integrons and 18 Plasmid replicons. A total of 48 Klebsiella isolates were identified. Multidrug resistance was observed in 23% of isolates. ESBL, AmpC and MBL resistance genes were detected in 21%, 6% and 4% of isolates, respectively. Integrons [Int2 gene] and plasmids [Y and IncI genes] were identified in 6% and 8% of isolates.The study highlights the existence of ESBL, AmpC and MBL producing Klebsiella isolates with certain strains carrying mobile genetic elements  in healthy livestock and poultry as reservoirs and probable disseminators of resistance, thus imposing public health threat. Prudent use of antimicrobials and continuous intensified surveillance in animal sector is crucial to limit the spread of such emerging resistant traits.

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