Expression profile of the myoglobin gene in indigenous native chicken

DOI: 10.18805/ijar.B-3596    | Article Id: B-3596 | Page : 852-855
Citation :- Expression profile of the myoglobin gene in indigenous native chicken.Indian Journal Of Animal Research.2019.(53):852-855
Athe Rajendra Prasad, N. Govardhana Sagar, R.N. Chatterjee, Chandan Paswan, S.P. Yadav and T.K. Bhattacharya bhattacharyatk@gmail.com
Address : ICAR-Directorate of Poultry Research, Hyderabad-500 030, Telangana, India.
Submitted Date : 12-03-2018
Accepted Date : 9-07-2018

Abstract

In the present study, we quantified myoglobin (Mb) gene expression in two different indigenous chicken breeds, namely Ghagus and Aseel to discern tissue specific expression pattern in different age groups. Quantitative real-time PCR assay was developed for measurement of the Mb mRNA expression in different tissues in chickens of different age groups (day old and day 28). Overall, the Mb mRNA level displayed a significant difference among all the tissues investigated in the study. Results exhibited that, bursa and heart tissues relatively had the highest expression of Mb related to the other tissues (P<0.05). Further, in gizzard and bursa, a significant difference was found in the expression between both the breeds studied. It is concluded that the expression of Mb gene varied significantly among different tissues and between age groups in chicken.

Keywords

Different tissues mRNA expression levels Myoglobin Native chicken

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