Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 53 issue 6 (june 2019) : 704-710

Effect of optimization of the levels of dissolved oxygen in semen extender on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa

Abhishek Kumar, J.K. Prasad, A.R. Mustapha, R. Katiyar, G.K. Das, S.K. Ghosh, M.R. Verma
1Division of Animal Reproduction, Division of Livestock Economics and Statistics, ICAR-Indian Veterinary Research Institute, Izatnagar - 243 122, Bareilly, Uttar Pradesh, India.
Cite article:- Kumar Abhishek, Prasad J.K., Mustapha A.R., Katiyar R., Das G.K., Ghosh S.K., Verma M.R. (2018). Effect of optimization of the levels of dissolved oxygen in semen extender on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa. Indian Journal of Animal Research. 53(6): 704-710. doi: 10.18805/ijar.B-3585.
The objective of present study was to investigate the effects of two different levels of dissolved oxygen (DO) i.e. 4 and 8 ppm in the extenders on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa following cryopreservation. Only those ejaculates that had a mass activity of ³ 3+ and individual progressive motility of  ³ 70% were utilized. Each of the ejaculates were divided and extended to have 80×106 spermatozoa/ mL of extender in three groups i.e. Group I (control), Group II (4 ppm) and Group III (8 ppm). Progressive motility (%) was higher (p<0.05) in Group II and III as compared to Group I at both pre-freeze and post-thaw stage. Per cent live sperm and HOST reactive sperm were higher (p<0.05) in Group II and in Group II and III as compared to Group I at pre-freeze and post-thaw stage, respectively. Sperm abnormality (%) was lower (p<0.05) in Group II at post-thaw stage. Per cent acrosome intact sperm did not differ (p>0.05) among the groups at pre-freeze stage, but, were higher (p<0.05) in Group II and III as compared to Group I at post-thaw stage. In conclusion, cryopreservation of semen having DO level of 4 ppm in semen extender yielded better results followed by 8 ppm in terms of semen quality parameters and functional membrane integrity of crossbred bull spermatozoa.
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