Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 53 issue 6 (june 2019) : 704-710

Effect of optimization of the levels of dissolved oxygen in semen extender on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa

Abhishek Kumar, J.K. Prasad, A.R. Mustapha, R. Katiyar, G.K. Das, S.K. Ghosh, M.R. Verma
1Division of Animal Reproduction, Division of Livestock Economics and Statistics, ICAR-Indian Veterinary Research Institute, Izatnagar - 243 122, Bareilly, Uttar Pradesh, India.
Cite article:- Kumar Abhishek, Prasad J.K., Mustapha A.R., Katiyar R., Das G.K., Ghosh S.K., Verma M.R. (2018). Effect of optimization of the levels of dissolved oxygen in semen extender on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa. Indian Journal of Animal Research. 53(6): 704-710. doi: 10.18805/ijar.B-3585.

ABSTRACT

The objective of present study was to investigate the effects of two different levels of dissolved oxygen (DO) i.e. 4 and 8 ppm in the extenders on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa following cryopreservation. Only those ejaculates that had a mass activity of ³ 3+ and individual progressive motility of  ³ 70% were utilized. Each of the ejaculates were divided and extended to have 80×106 spermatozoa/ mL of extender in three groups i.e. Group I (control), Group II (4 ppm) and Group III (8 ppm). Progressive motility (%) was higher (p<0.05) in Group II and III as compared to Group I at both pre-freeze and post-thaw stage. Per cent live sperm and HOST reactive sperm were higher (p<0.05) in Group II and in Group II and III as compared to Group I at pre-freeze and post-thaw stage, respectively. Sperm abnormality (%) was lower (p<0.05) in Group II at post-thaw stage. Per cent acrosome intact sperm did not differ (p>0.05) among the groups at pre-freeze stage, but, were higher (p<0.05) in Group II and III as compared to Group I at post-thaw stage. In conclusion, cryopreservation of semen having DO level of 4 ppm in semen extender yielded better results followed by 8 ppm in terms of semen quality parameters and functional membrane integrity of crossbred bull spermatozoa.

REFERENCES

  1. Alvarez, J.G. and Storey, B.T. (1985). Spontaneous lipid-peroxidation in rabbit and mouse epididymal spermatozoa dependence of rate on temperature and oxygen concentration. Biol Reprod., 32:342–51.
  2. Asadpour, R., Jafari, R. and Tayefi-Nasrabadi, H. (2012). The effect of antioxidant supplementation in semen extenders on semen quality and lipid peroxidation of chilled bull spermatozoa. Iran J Vet Res., 13 (3): 40.
  3. Balamurugan, B., Ghosh, S.K., Lone, S.A., Prasad, J.K ., Das G.K ., Katiyar, R., Mustapha, A.R., Kumar, A. and Verma, M.R . (2018). Partial deoxygenation of extender improves sperm quality, reduces lipid peroxidation and reactive oxygen species during cryopreservation of buffalo (Bubalus bubalis) semen. Anim. Reprod. Sci. (Article in press).189: 60-68.
  4. Bucak, M.N., Atessahin, A., Varis¸li, O., Yuce, A., Tekin, N. and Akcay, A. (2007). The influence of trehalose, taurine, cysteine and hyaluronan on ram semen. Microscopic and oxidative stress parameters after freeze-thawing process. Theriogenology, 67(5): 1060–1067.
  5. Bucak, M.N., Sariozkan, S. and Tuncer,et al. (2010). The effect of antioxidants on post-thawed Angora goat (Capra hircusancryrensis) spermparameters, lipid peroxidation and antioxidant activities. Small Rumin Res., 89 (1): 24–30.
  6. Bucak, M.N., Tuncer, P.B., Sariozkan, S. and Ulutas, P.A. (2009). Comparison of the effects of glutamine and an amino acid solution on post-thawed ram sperm parameters, lipid peroxidation and anti-oxidant activities. Small Rumin Res., 81(1): 13–17.
  7. Gibb, Z., Lambourne, S.R. and Aitken, R.J. (2014). The paradoxical relationship between stallion fertility and oxidative stress. Biol Reprod., 91:77.
  8. Gundogan, M., Avdatek, F. and Yen, D. (2011). Effect of extenders on motility, morphology and osmotic resistance parameters of ram sperm during liquid storage. Rev. Méd. Vét., 162: 546-551.
  9. Jeyendran, R.S.et al. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil., 70:219–228.
  10. Juyena, N. S. (2011). Protein profiles and biochemical characteristics of semen: Influence on frozen thawed spermatozoa quality in rams (Ovis Aries) and Alpacas (Vicugna Aacos). PhD Thesis, Faculty of Veterinary Medicine, Department of Clinics Veterinary Science. University of Paduva, Italy, pp. 123-173.
  11. Koppers, A.J., De Iuliis, G.N., Finnie, J.M., McLaughlin, E.A. and Aitken, R.J. (2008). Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. J Clin Endocrinol Metab., 93: 3199 – 3207.
  12. Kumar, S.A. and Krupakaran R.P. (2014). Comparative study on physico- morphological characteristics of semen from murrah buffaloes and jersey crossbred cattle. Cibtech Journal of Bio-Protocols., 3 (3):12-14.
  13. Kumar, U., Gawande, A.P., Sahatpure, S.K., Patil, M.S., Lakde, C.K., Bonde, SW., Borkar, P.L., Poharkar, A.J. and Ramteke, B.R. (2015). Assessment of semen quality in pure and crossbred Jersey bulls. Vet World, 8(10): 1266-1272.
  14. Lessard, C., Parent, S., Leclerc, P., Bailey, J.L. and Sullivan, R. (2000). Cryopreservation alters the level of the bull sperm surface protein P25b. J Androl., 21: 700 –7. 
  15. Loyi ,T.( 2008). Comparative studies on electrolytes and total seminal plasma protein between freezable and non freezable crossbred bull semen. M. V. Sc thesis submitted to the Deemed University, Indian Veterinary Research Institute (IVRI), Izatnagar, India.
  16. Mandal, D.K., Tyagi, S. and Mathur, A.K. (2005). Semen production performance of sahiwal bulls. Indian. J. anim. Sci., 75(1):17-19.
  17. Medeiros, C.M., Forell, F., Oliveira, A.T. and Rodrigues, J.L. (2002). Current status of sperm cryopreservation: why isn’t better. Theriogenology, 57:327–344.
  18. Mittal, P.K., Anand, M., Madan, A.K., Yadav, S. and Kumar, J. (2014). Antioxidative capacity of vitamin E, vitamin C and their combination in cryopreserved Bhadavari bull semen. Vet World, 7(12): 1127-1131.
  19. Mustapha, A. R. (2017). Studies on partial deoxygenation of extender on freezability of crossbred bull spermatozoa. Ph.D thesis submitted to the Deemed University, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India.
  20. Nakata, K., Yamashita, N., Noda, Y. and Ohsawa, I.(2015). Stimulation of human damaged sperm motility with hydrogen molecule. Med Gas Res., 5:2.
  21. Odhiambo, J. F., DeJarnette, J.M., Geary, T. W., Kennedy, C. E., Suarez, S. S., Sutovsky, M. and Sutovsky, P. (2014). Increased Conception Rates in Beef Cattle Inseminated with Nanopurified Bull Semen. Biol Reprod., 91(4):97, 1–10.
  22. Pande, M., Srivastava, N., Rajoriya, J.S., Ghosh, S.K., Prasad, J.K. and Ramteke, S.S. (2015). Effects of degasified extender on quality parameters of cryopreserved bull spermatozoa. International Journal of Veterinary Sciences Research, 1(3): 70-78.
  23. Patel, B.R. and Siddiquee, G.M. (2013). Physical and morphological characteristics of Kankrej bull semen. Vet World, 6(7): 405-408.
  24. Rajoriya, J.S., Prasad, J.K., Ghosh, S.K., Kumar, A., Ramteke, S.S., Singh, M. and Kumar ,S. (2015). Effect of peak winter and summer season on quality and freezability of Tharparkar bull semen. Indian J. Anim. Res., 49 (2): 265-268.
  25. Reddy, N.S.S., Mohanarao, G.J. and Atreja, S.K. (2010). Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalis bubalis) sperm quality following cryopreservation. Anim. Reprod. Sci., 119: 183–190.
  26. Salisbury, G.W., Van Demark, N.L. and Lodge, J.R. (1978). Physiology of reproduction, seminal plasma and sperm. Biol Reprod., 63: 1058-1066.
  27. Satish Kumar. (2007). Advances in semen preservation: concept and its minimization. XXIII Annual convention of ISSAR and national symposium .Bhubaneswar. pp: 207.
  28. Selvaraju S, Ravindra, J.P., Ghosh, J., Gupta, P.S.P. and Suresh, K.P. (2008). Evaluation of sperm functional attributes in relation to in vitro sperm zona pellucida binding ability and cleavage rate in assessing frozen thawed buffalo (Bubalus bubalis) semen quality. Anim. Reprod. Sci., 106: 311–321.
  29. Shannon, P. and Curson, B. (1984). Effect of storage temperature on the viability and fertility of bovine sperm diluted and stored in Caprogen. New Zeal J Agr Res., 27: 173-177.
  30. Shelke, V.B. and Dhami, A.J. (2001). Comparative evaluation of physico-morphological attributes and freezability of semen of Gir cattle (Bos indicus) and Jafarabadi buffalo (Bubalus bubalis) bulls. Indian J. Anim. Sci., 71: 319-324.
  31. Sikka, S.C., Rajasekaran, M. and Hellstrom, W.J.G. (1995). Role of oxidative stress and antioxidants in male infertility. J Androl., 16 (6): 464–468.
  32. Snedecor G W and Cochran W G. (1989). Statistical Methods. 8th edition Iowa State University Press, Ames, Iowa, Reprinted in 1994. East-West Press Pvt. Ltd., New Delhi.
  33. Swanson, E. W. and Bearden, H. J. (1951). An eosin-nigrosin stain for differentiating live and dead bovine spermatozoa. J. Anim. Sci., 10: 981.
  34. Thundathil, J., Andrzej, T.P., Albert, D.B. and Reuben, J.M. (1998). Plasma membrane and acrosomal integrity in bovine spermatozoa with knobbed acrosome defect. Theriogenology, 58: 87-102.
  35. Uysal O. and Bucak, M.N. (2007). Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. Acta Veterinaria Brno., 76(3): 383–390.
  36. Uysal, O., Buck, M.N., Yavas, I. and Varisli., O. (2007). Effect of various antioxidants on the Quality of frozen thawed bull semen. J. Anim.Vet. Ad., 6(12):1362-1366.
  37. Watson, P.F. (1975). Use of Giemsa stain to detect changes in the acrosome of frozen ram spermatozoa. Vet. Record., 97:12-15. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)