Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 53 issue 6 (june 2019) : 716-719

Expression study of fertility related genes with respect to sperm motility, HOST and acrosome integrity in bovine semen

I. Borgohain, D. Dutta, P. Borah, D. Borah
1Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Khanapara-781 022, Assam, India.
Cite article:- Borgohain I., Dutta D., Borah P., Borah D. (2018). Expression study of fertility related genes with respect to sperm motility, HOST and acrosome integrity in bovine semen. Indian Journal of Animal Research. 53(6): 716-719. doi: 10.18805/ijar.B-3576.

ABSTRACT

The present study was conducted for molecular evaluation of bull semen and to study its relationship with different semen characteristics. Fresh semen samples were collected from six breeding bulls. A total of six ejaculates were collected from each of six healthy breeding Jersey bulls at 4 days interval. Immediately after collection, the samples were subjected to physio-morphological and biomolecular evaluation. Percentage of Hypo-osmotic Swelling Test (HOST) positive spermatozoa and acrosome-intact sperms increased with an increase in initial sperm motility. Expression of Chaperonin Containing T-Complex protein 1, subunit 8 (CCT8) gene was found to be negatively correlated with sperm motility, whereas the expression of  Adenylate Kinase 1 (AK1) gene did not show any significant relationship with sperm motility.

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