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Indian Journal of Animal Research

  • Chief EditorM. R. Saseendranath

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Research Article

Indian Journal of Animal Research, volume 54 issue 10 (october 2020) : 1241-1245

Real-Time PCR Detection of Mycobacterium bovis in Blood and Lymph Node Aspirates of Bovines Positive in Tuberculosis Screening Tests

G.S. Sidhu, D. Narang, G. Filia, A. Singh, S.T. Singh, M. Chandra, N.S. Sharma
1Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
Cite article:- Sidhu G.S., Narang D., Filia G., Singh A., Singh S.T., Chandra M., Sharma N.S. (2020). Real-Time PCR Detection of Mycobacterium bovis in Blood and Lymph Node Aspirates of Bovines Positive in Tuberculosis Screening Tests. Indian Journal of Animal Research. 54(10): 1241-1245. doi: 10.18805/ijar.B-3573.

ABSTRACT

Bovine tuberculosis is a chronic infectious disease affecting broad range of mammalian hosts. ESAT-6 is a low molecular weight immunodominant protein coded by esxA gene located on RD1 region of genome and is responsible for virulence of Mycobacterium bovis. Out of 200 animals screened for bovine Tuberculosis (bTB), 38 animals (19%) were found positive for Comparative intradermal tuberculin test (CITT) (32 cattle, 6 buffaloes) and 41 animals were tested positive by IFN-g assay (29 cattle, 12 buffaloes). DNA extraction of blood (n=200) and lymph node aspirates (n=48) was done. Among 200 blood samples targeted for esxA (ESAT-6) gene, three samples (1.5%) whose CT was between 23-34 were considered positive by real-time PCR. Out of 48 animals (lymph node aspirates) that were positive either by CITT or IFN-g, one sample (2.08%) whose CT was between 23-34 were considered positive by real-time PCR. Remaining samples whose CT values were equal to or greater than 35 were considered negative. The sensitivity of esxA was 8 pg/ìl by real time PCR.

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