Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 53 issue 11 (november 2019) : 1462-1468

Customising the requirement of fibrolytic enzymes to improve feeding value of sorghum stover, ragi straw and groundnut haulms

C. Valli, Yancy Mary Issac, R. Kavitha
1Department of Animal Nutrition, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, Tamil Nadu, India.
Cite article:- Valli C., Issac Mary Yancy, Kavitha R. (2019). Customising the requirement of fibrolytic enzymes to improve feeding value of sorghum stover, ragi straw and groundnut haulms. Indian Journal of Animal Research. 53(11): 1462-1468. doi: 10.18805/ijar.B-3557.

ABSTRACT

A study was carried out to determine fibre and non starch polysaccharide fractions of sorghum stover, ragi straw and groundnut haulms. Sorghum stover had the significantly highest fibre fractions (NDF, ADF, Cellulose, hemicelluloses and Lignin) and non starch polysaccharide fractions (Total, Soluble and Insoluble) compared to the other two crop residues. Enzyme activity assay of cellulase, hemicellulase, xylanase and pectinase revealed multiple activities in a single enzyme. In vitro trials were carried out to evolve substrate specific customized non - starch polysaccharidase mixture for sorghum stover, ragi straw and groundnut haulm. The first trial was conducted to locate the range of enzymes required for maximum sugar release, followed by another in vitro trial to precisely identify the enzymes needed for respective substrates and the third one was to identify the inclusion level of these enzymes in combination to sorghum stover or ragi straw or groundnut haulm. All these trials were conducted in duplicate in three runs. These experiments established that each gram of sorghum stover and ragi straw requires 1200 U of Cellulase, 120 U of Xylanase and 700 U of Pectinase for maximum hydrolysis and each gram of groundnut haulm requires 1600 U of Cellulase, 100 U of Xylanase and 600 U of Pectinase for maximum hydrolysis.

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