Banner
Current IssueEditorial BoardOnline First Articles
Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Research Article

Indian Journal of Animal Research, volume 53 issue 4 (april 2019) : 469-475

Cholesterol-lowering effects of Bacillus coagulans B37 and Bacillus pumilus B9 strains in a rat animal model

Lopamudra Haldar, D.N. Gandhi
1Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal-132 001, Haryana, India.
Cite article:- Haldar Lopamudra, Gandhi D.N. (2018). Cholesterol-lowering effects of Bacillus coagulans B37 and Bacillus pumilus B9 strains in a rat animal model. Indian Journal of Animal Research. 53(4): 469-475. doi: 10.18805/ijar.B-3549.

ABSTRACT

An experiment was conducted for the 56 d period in thirty six adult male albino Wister rats divided equally into four groups. After 7 d adaptation period, all the groups were fed with cholesterol enriched diet for 14 d.  Thereafter, one group (T1) was fed on sterile skim milk along with cholesterolemic diet for the next 21 d. The two experimental groups (T2 and T3) received cholesterolemic diet plus spore biomass of B. coagulans B37 and B. pumilus B9, respectively, suspended in sterilized skim milk @ 8-9 log cfu/ ml for the next 21 d. The control group was supplied with clean water along with cholesterolemic diet for 21 d. All the animals were fed on the basal diet along with water during a 14 d post-treatment period. After the adaptation period, the blood samples were collected weekly from the experimental rats up to 42 d, and plasma separated, and subjected to the determination of different plasma lipid fractions. The oral administration of both B. coagulans B37 and B. pumilus B9 resulted a decrease  (P<0.05) in plasma cholesterol, low density lipoprotein (LDL)-cholesterol concentrations and atherogenic index in the experimental rats. 

REFERENCES

  1. Atlas, R.M. (2004). Handbook of Microbiological Media, 3rd edn., Taylor & Francis, Boca Raton.
  2. Bader, J., Albin, A., Stahl, U. (2012). Spore-forming bacteria and their utilisation as probiotics. Beneficial Microbes, 3: 67-75.
  3. Bliznakov, E.G. (2002). Lipid-lowering drugs (statins), cholesterol, coenzyme Q10. The baycol case-a modern Pandoras box. Biomedical and Pharmacology, 56: 56-59.
  4. Choi, C.H., Kwon, J.G., Kim, S.K., Myung, S.J., Park, K.S., Sohn, C.I., Rhee, P.L., Lee, K.J., et al., (2015). Efficacy of combination therapy with probiotics and mosapride in patients with IBS without diarrhea: a randomized, double-blind, placebo-controlled, multicenter, phase II trial. Journal of Neurogastroenterology and Motility, 27: 705-716.
  5. Cutting, S.M. (2011). Bacillus probiotics. Food Microbiology, 28 : 214-220. 
  6. Ejtahed, H.S., Mohtadi-Nia, J., Homayouni-Rad, A., Niafar, M., Asghari-Jafarabadi, M., et al., (2011). Effect of probiotic yogurt containing Lactobacillus acidophilus and Bifidobacterium lactis on lipid profile in individuals with type 2 diabetes mellitus. Journal of Dairy Science, 94: 3288-3294.
  7. Friedewald, W.R.R., Levy, I., Frederickson, D.S. (1972). Lipoproteins in serum. Clinical Chemistry, 18: 499-502.
  8. Haldar, L., Gandhi, D.N., Majumdar, D., De, S. (2015). Characterization of indigenous Bacillus coagulans isolated from cattle and buffalo milk. International Journal of Microbiological Research, 7: 686- 691.
  9. Huang, W.C., Chen, Y.M., Kan, N.W., Ho, C.S., Wei, L., Chan, C.H., Huang, H.Y., Huang, C.C. (2015). Hypolipidemic effects and safety of Lactobacillus reuteri 263 in a hamster model of hyperlipidemia. Nutrients, 7: 3767-3782. 
  10. Ishimwe, N., Daliri, E.B., Lee, B.H., Fang, F., Du, G. (2015). The perspective on cholesterol-lowering mechanisms of probiotics. Molecular Nutrition and Food Research, 59: 94-105. 
  11. Jeong, J.S., Kim, I.H. (2014). Effect of Bacillus subtilis C-3102 spores as a probiotic feed supplement on growth performance, noxious gas emission, and intestinal microflora in broilers. Poultry Science, 93: 3097-3103.
  12. Kawase, M., Hashimoto, H., Hosoda, M., Morita, H., Hosono, A. (2000). Effect of administration of fermented milk containing whey protein concentrate to rats and healthy men on serum lipids and blood pressure. Journal of Dairy Science, 83: 255-263.
  13. Larsen, N., Thorsen, L., Kpikpi, E.N., Stuer-Lauridsen, B., Cantor, M.D., Nielsen, B. et al., (2014). Characterization of Bacillus spp. strains for use as probiotic additives in pig feed. Applied Microbiology and Biotechnology, 98: 1105-1118.
  14. Lefevre, M., Racedo, S.M., Ripert, G., Housez, B., Cazaubiel, M., Maudet, C., Jüsten, P., et al., (2015). Probiotic strain Bacillus subtilis CU1 stimulates immune system of elderly during common infectious disease period: a randomized, double-blind placebo-controlled study. Immunity and Ageing, 12: 24.
  15. Lye, H.S., Rusul, G., Liong, M.T. (2010). Removal of cholesterol by lactobacilli via incorporation and conversion to coprostanol. Journal of Dairy Science, 93: 1383-1392.
  16. Makhal, S., Prabha, S., Mandal, S., Kanawjia, S.K. (2004). Milk and milk products: an intricate dilemma in relation to cardio vascular diseases. Indian Dairyman, 56: 49-59.
  17. Martinez, R.C., Bedani, R., Saad, S.M. (2015). Scientific evidence for health effects attributed to the consumption of probiotics and prebiotics: an update for current perspectives and future challenges. British Journal of Nutrition, 114: 1993-2015. 
  18. Mohania, D., Kansal, V.K., Shah, D., Nagpal, R., Kumar, M., Gautam, S.K., et al (2013). Therapeutic effect of probiotic dahi on plasma, aortic, and hepatic lipid profile of hypercholesterolemic rats. Journal of Cardiovascular Pharmacology and Therapeutics, 18: 490-497.
  19. Nam, Y., Jung, H., Karuppasamy, S., Lee, J.Y., Kang, K.D., Hwang, K.Y., Seong, S.I., Suh, J.G. (2012). Anti-hyperlipidemic effect of soybean extract fermented by Bacillus subtilis MORI in db/db mice. Laboratory Animal Research, 28: 123-129.
  20. Paik, H.D., Park, .JS., Park, E. (2005). Effects of Bacillus polyfermenticus SCD on lipid and antioxidant metabolisms in rats fed a high-    fat and high-cholesterol diet. Biological and Pharmaceutical Bulletin, 28: 1270-1274.
  21. Pavloviæ, N., Stankov, K., Mikov, M. (2012). Probiotics—interactions with bile acids and impact on cholesterol metabolism. Applied Biochemistry And Biotechnology, 168: 1880-1895. 
  22. Peng, H., Wang, J.Q., Kang, H.Y., Dong, S.H., Sun, P., Bu, D.P., Zhou, L.Y. (2012). Effect of feeding Bacillus subtilis natto fermentation product on milk production and composition, blood metabolites and rumen fermentation in early lactation dairy cows. Journal of Animal Physiology and Animal Nutrition (Berlin), 96: 506-512.
  23. Pereira, D.I., Gibson, G.R. (2002). Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. Applied and Environmental Microbiology, 68: 4689-4693.
  24. Rana, R., Gandhi, D.N. (2000). Effect of basal medium and pH on the growth of Lactobacillus acidophilus. Indian Journal of Dairy Science, 53: 338-342.
  25. Raut, S.V., Pingle, Y.A. (2010). Screening and characterization of antimicrobial substances produced by Bacillus species. Journal of Pure and Applied Microbiology, 4: 321-331.
  26. Rerksuppaphol, S., Rerksuppaphol, L. (2015). A randomized double-blind controlled trial of Lactobacillus acidophilus Plus Bifidobacterium bifidum versus Placebo in Patients with Hypercholesterolemia. Journal of Clinical and Diagnostic Research, 9: KC01-4.
  27. Roberfroid, M. (2007). Prebiotics: The concept revisited. Journal of Nutrition, 137: 830S-837S.
  28. Ryan, J.J., Hanes, D.A., Schafer, M.B., Mikolai, J., Zwickey, H. (2015). Effect of the probiotic saccharomyces Boulardii on cholesterol and lipoprotein particles in Hypercholesterolemic adults: A Single-Arm, Open-Label Pilot Study. Journal of Alternative and Complementary Medicine, 21: 288-293.
  29. Sharma, S., Kurpad, A.V., Puri, S. (2016). Potential of probiotics in hypercholesterolemia: A meta-analysis. Indian Journal of Public Health, 60: 280-286. 
  30. Shimizu, M., Hashiguchi, M., Shiga, T., Tamura, H.O., Mochizuki, M. (2015). Meta-analysis: Effects of probiotic supplementation on lipid profiles in normal to mildly hypercholesterolemic individuals. PLoS One, 10:e0139795. 
  31. Singh, T.P., Malik, R.K., Katkamwar, S.G., Kaur, G. (2015). Hypocholesterolemic effects of Lactobacillus reuteri LR6 in rats fed on high-    cholesterol diet. International Journal of Food Sciences and Nutrition, 66: 71-75.
  32. Song, M., Park, S., Lee, H., Min, B., Jung, S., Park, S., Kim, E., Oh, S. (2015). Effect of Lactobacillus acidophilus NS1 on plasma cholesterol levels in diet-induced obese mice. Journal of Dairy Science, 98: 1492-1501.
  33. SYSTAT 6.0.1 (1996). Statistical Software Package. Chicago (IL): SPSS, Inc., USA.
  34. Urgesi, R., Casale, C., Pistelli, R., Rapaccini, G.L., de Vitis, I. (2014). A randomized double-blind placebo-controlled clinical trial on efficacy and safety of association of simethicone and Bacillus coagulans (Colinox®) in patients with irritable bowel syndrome. European Review for Medical and Pharmacological Sciences, 18: 1344-1353.
  35. Usman, Hosono, A. (2000). Effect of administration of Lactobacillus gasseri on serum lipids and fecal steroids in hypercholesterolemic rats. Journal of Dairy Science, 83: 1705-1711.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)