Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 53 issue 5 (may 2019) : 648-654

Nanoparticle based Brucella melitensis vaccine induced oxidative stress acts in synergism to immune response 

Amit Kumar, V. K. Gupta, Anu Rahal, Rajesh Mandil, A.K.Verma, S. K. Yadav
1Department of Veterinary Microbiology, College of Veterinary Sciences, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura-281 001, Uttar Pradesh, India.
Cite article:- Kumar Amit, Gupta K. V., Rahal Anu, Mandil Rajesh, A.K.Verma, Yadav K. S. (2018). Nanoparticle based Brucella melitensis vaccine induced oxidative stress acts in synergism to immune response. Indian Journal of Animal Research. 53(5): 648-654. doi: 10.18805/ijar.B-3548.

ABSTRACT

Brucella melitensis, mainly responsible for human brucellosis, is considered as biological warfare. Being intracellular in nature Brucella induces oxidative stress due to bacterial invasion in organs like spleen and lymph nodes. The present study was aimed to evaluate the specific oxidative stress responses in erythrocytes and tissues induced by intranasal inoculation of killed Brucella whole cell protein antigen coupled with sodium acrylate nanoparticle adjuvant. Glutathione mediated nonenzymic antioxidant defense system and catalase have been observed to play a critical role in intracellular antioxidant defenses in vaccinated animals and also in maintaining an excellent milieu to induce an active immune potential. The antioxidant defenses in erythrocytes, liver, kidneys, lungs and spleen of vaccinated mice also maintained excellent redox homeostasis required for quality and effective protection without inflammatory pathophysiology.

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