Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 52 issue 10 (october 2018) : 1469-1475

Toxicological evaluation and effects of oganophosphate compounds on hematological profile of juvenile common carps (Cyprinus carpio var. Communis)

Imtiyaz Qayoom, Masood H. Balkhi, Feroz A. Shah, Bilal A. Bhat
1Faculty of Fisheries, Sher e Kashmir University of Agricultural Sciences and Technology of Kashmir, Rangil Ganderbal-190006, Jammu and Kashmir, India.
Cite article:- Qayoom Imtiyaz, Balkhi H. Masood, Shah A. Feroz, Bhat A. Bilal (2018). Toxicological evaluation and effects of oganophosphate compounds on hematological profile of juvenile common carps (Cyprinus carpio var. Communis). Indian Journal of Animal Research. 52(10): 1469-1475. doi: 10.18805/ijar.B-3526.

ABSTRACT

Present study was carried out to investigate the alterations in the haematological profile of juvenile common carp (Cyprinus carpio var. communis) against acute toxicity of organophosphorous compounds, dimethoate and chlorpyrifos. The fish were divided into 5 different experimental groups for range finding tests on the basis of which the doses for definitive tests were selected. The acute bioassay toxicity tests were carried out in triplicates for 96 hours with a control group run parallel to the experiment. Data obtained was analyzed as per Finney’s probit to determine LC50 values. The mean lethal concentration was found 1.1ppm and 3.8ppb respectively for dimethoate and chlorpyrifos. The samples investigated for various haematological parameters such as haemoglobin concentration (Hb), Red blood cell (TEC) and White blood cell (TLC & DLC) counts the heamatocrit (Ht) level, the mean corpuscular volume (MCV) and the mean corpuscular haemoglobin (MCH). Packed cell volume (PCV) and Erythrocyte sedimentation rate (ESR) showed a negative impact of pesticide treated fishes when compared with control. Hb, TEC and Ht were found lower while as ESR and TLC were recorded higher than the control specimens. MCV and MCH showed statistically insignificant differences when compared with control. In DLC, monocyte lymphocyte, neutrophil and basophils were found increased while as eosinophil count was found unaffected than the normal. Studies suggested that the pesticides are potential toxicants for common carps among which chlorpyrifos was found to elicit profound changes intense than dimethoate.

REFERENCES

  1. Abdel-Megeed, A. and Aly El-Nakieb, F. (2008). Dimethoate degradtion by EM. Terrestrial and Aquatic Environmental Toxicology 2 (1), 1-4.
  2. Adoni, A.D., Joshi, G., Ghosh, K., Chourasia, S.K., Vaishya, A.K., Yadav, M. and Verma, H.G. (1985). Workbook on limnology. Department of Botany, Dr. Hari Singh Gour Vishwadidyalaya, Sagr Publishers, Sagar Madhya Pradesh.
  3. Ahamad, Z. (2011). Acute toxicity and haematological changes in common carp (Cyprinus carpio) caused by diazinon exposure. African Journal of Biotechnology. 10(63):13852-13859.
  4. Abdullah Khalid Al-Ghanim. (2013). Study on the effect of diazinon on haematological indices in common carp (Cyprinus carpio L.). Life Science Journal 10(10): 327-332.
  5. Ali, H.A.J. and Rani, V.J. (2009). Effect of phosalone on haematological indices in the Tilapia, Oreochromis mossambicus. Turkey Journal of Veterinary and Animal Sciences 33(5): 407-411.
  6. APHA (2005). Standards Methods for the Examination of Water and Waste Water. 21stEdn., Washington D.C.
  7. Asadi, F., Rostami, A., Pourkabir, M., Shahriari, A. (2007). Serum lipid and lipoprotein profile of Asian tortoise (Agrionemyhorsfieldi) in prehibernation state. Comparative Clinical Pathology, 16: 193-195.
  8. Bahmani, M., Kazemi, R. and Donskaya, P. (2001). A comparative study of some hematological features in young reared sturgeons (Acipenser persicus and Huso huso). Fish Physiol. Biochem. 24:135-140.
  9. Banaee, M., Mirvagefei, A.R., Rafei, G.R. and Majazi Amiri, B. (2008). Effect of sub-lethal diazinon concentrations on blood plasma biochemistry. International Journal of Environmental Research 2(2):189-198.
  10. Bhatia NP, Sanhu GS, Johal MS (2004). Haematological alterations in Heteropneuses fossilis upon exposure to endosulfan. Pollut. Res. 23(4): 633-636.
  11. Blaxhall, P.C. and Daisley, K.W. (1973). Routine haematological methods for use with fish blood. J. Fish Biol. 5: 771-781.
  12. Campagna, A.F., Eler, M.N., Espíndola, E.L.G., Senhorini, J.A., doRêgo, R.F.and Silva, L.O.L. (2006). Dimethoate 40% Organophosphorous pesticide toxicity in Prochilodus Lineatus (Prochilodontidae, Characiformes) eggs and larvae. Brazilian Journal of Biology. 66(2B): 633-640.
  13. Çelik, E. (2004). Blood chemistry (electrolytes, lipoproteins and enzymes) values of black scorpion fish (Scorpaenaporcus Linneaus 1758) in the Dardanelles, Turkey Journal of Biological Science. 4: 716-719.
  14. Das, B.K. and Mukherjee, S.C. (2001). Effect of Nuvan on haematology of rohu Labeo rohita (Ham.). Indian Journal of Fisheries 48 (1): 85-92.
  15. Deka, C. and Dutta, K. (2012). Effects of cypermethrin on some haematological parameters in Heteropneustes fossilis (Bloch). The Bioscan. 7(2): 221-223.
  16. Extoxnet, (1996). Extension Toxicology Network Pesticide Information Profiles. http://extoxnet.orst.edu/pips/chlorpyr.htm.
  17. Fazio, F., Saoca, C., Piccione, G., Kesbiç, O. S. and Acar, U. (2016.) Comparative study of some hematological and biochemical parameters of Italian and Turkish farmed rainbow trout Oncorhynchus mykiss (Walbaum, 1792). Turkish Journal of Fisheries and Aquatic Sciences 16: 715-721.
  18. Finney, D.T.(1971). Probit Analysis; Cambridge University Press: Cambridge, UK.
  19. Ganeshwade, R.M. 2011. Biochemical changes induced by Dimethoate in the liver of fresh water fish Puntius ticto (Ham). Biological Forum - An International Journal 3(2): 65-68.
  20. Ganeshwade, R.M. (2012). Biochemical changes induced by Dimethoate (Rogor 30% EC) in the gills of fresh water fish Puntius ticto (Hamilton). Journal of Recent Trends in Bioscience 2(1): 58-64.
  21. Gill, T.S., Pant, J.C. (1987). Haematological and pathological effects of chronic toxics in freshwater fish, Barbus conchomius. Ham. Water Air Soil Poll. 35: 241-250.
  22. Halappa, R. and David, M. (2009). Behavioural responses of the freshwater fish, Cyprinus carpio (Linnaeus) following sub-lethal exposure to chlorpyrifos. Turkish Journal of Fisheries and Aquatic Sciences 9: 233-238.
  23. Haniffa, M.A. (1990). Haematological effects of textile mills effluents on freshwater fish, Oreochromis mossambicus. Environ. Res. 17:191.
  24. Hudson, I. and Hay, C.F. (1991). Antibody Interaction with Antigen. Practical Immunology. IIIrd edition. Oxford: Blackwell Scientific Publication, pp. 208-260.
  25. Janardana, R.S. and Vineela, D. (2013). Impact of azodrin on haematological indices and behavioural responses of carp fish. Electronic Journal of Biosciences 1(3): 54-62.
  26. Johal, M.S., Grewal, H. (2004). Toxicological study on the blood of Channa punctatus (Bloch) upon exposure to carbaryl. Pollut. Res. 23(1): 601- 606. 
  27. Karatas, T. (2016). Effects of deltamethrin on some haematological parameters of brown trout (Salmo trutta fario). Indian J. Anim. Res. 50(1): 89-92.
  28. Köprücü, S.O., Köprücü, K., Mevlüt, S., Ural, E., Ispir, U. and Pala, M. (2006). Acute toxicity of organophosphorous pesticide diazinon and its effects on behaviour and some hematological parameters of fingerling European catfish (Silurus glanis L.). Pesticide Biochemistry and Physiology. 86: 99-105.
  29. Laabs, V., Wehrhan, A., Pinto, A., Dores, E., Amelung, W. (2007). Pesticide fate in tropical wetlands of Brazil: an aquatic microcosm study under semi-field conditions. Chemosphere. 67:975–989.
  30. Lakshmanan, S., Rajendiran, A. and Sivasubramaniyan, C. 2013. Studies on impact of dichlorovos on selected haematological parameters of freshwater fish, Oreochromis mossambicus (Peters). International Journal of Research in Biological Sciences. 3(1): 28-33.
  31. Lucky, Z. 1977. Methods for the diagnosis of Fish diseases. Amerno publishing Co., PVT, LTD, New Delhi, Bombay, Calcutta and New York.
  32. Nath, R. and Banerjee, V. (1996). Effect of pesticides methyl parathion and cypermethrin on the air breathing fish H. fossilis (Bloch). J. Environ. Ecol. 14(1): 163-165.
  33. Okechukwu, O.E., Usman, I.B. and Jehu, A. (2013). Investigation of acute toxicity of chlorpyrifos-ethyl on Clarias gariepinus-(Burchell, 1822) using some behavioural indices. International Journal of Basic and Applied Sciences. 2(2): 176-183.
  34. Qayoom, I. (2010). Residue Analysis of Pesticides from Dal Waters and Their Effect on Common Carp Fish, Cyprinus carpio var. communis. PhD Thesis submitted to SKUAST-K Shalimar Srinagar Jammu and Kashmir India.
  35. Rahman, M. Z., Hossain, Z. M.F., Mollah, A. and Ahmed, G. U. (2002). Effect of eiazinon 60 EC on Anabas testudineus, Channa punctatus and Barbodes gonionotus. NAGA, The ICLARM Quarterly. 25(2): 8-12.
  36. Reish, D.J and Oshida, R.S. (19870. Manual of methods in aquatic environment research. Part 10 short term static Bioassay. FAO Fish Tech. 247: 62.
  37. Sachar, A. and Raina, S. (2014). Haematological alterations induced by lindane in a fish, Aspidoparia morar. Global Journal of Biology, Agriculture and Health Sciences. 3(1):38-42.
  38. Sancho, E., Febrando, M.D. and Andreu, E. (2000). Sublethal effects of an organophosphate insecticide on the European eel Anguilla anguilla. Ecotoxicol. Environ. Safety. 36: 57-65.
  39. Schaperclaus, W. (1991). Fish Diseases. Volume 1. CRC Press; 5 edition Oxonian Press Pvt. Ltd., New Delhi. 
  40. Shamoushaki, M. N. M., Soltani, M., Kamali, A., Imanpoor, M. R., Sharifpour, I. and Khara, H. (2012). Effects of organophosphate, diazinon on some haematological and biochemical changes in Rutilus frisii kutum (Kamensky, 1901) male brood stocks. Iranian Journal of Fisheries Sciences. 11(1) 105-117.
  41. Sharmin, S., Abdus Salam, Md., Asadul Haque, Md. and Shahjahan, Md. (2014. Toxicity bioassay of organophosphorous pesticide malathion in common carp, Cyprinus carpio. Proceedings of 5th International Conference on Environmental Aspects of Bangladesh.
  42. Svoboda, Z., Lusková, V., Drastichová, J., Svoboda, M. and Zlábek, V. (2001). Effect of deltamethrin on haematological indices of common carp (Cyprinus carpio L.). Acta Vet. Brno. 72: 79-85.
  43. Verma, V.K. and Saxena, A. (2013). Investigations on the acute toxicity and behavioural alterations induced by the organophosphate pesticide, chlorpyrifos on Puntius chola (Hamilton-Buchanan). Indian J. Fish. 60(3): 141-145.
  44. Wast, N., Tiwari, K., Gupta, A.K., Prakash, M.M. and Gaherwal, S. (2015). Assessment of acute toxicity of chlorpyriphos 20% EC to the Guppy, Poecilia reticulata (Peters, 1859). Global Veterinaria 14(2): 239-243.
  45. Wendelaar, Bonga SE. (1997). The stress response in fish. Physiol Rev. 77:591-625.
  46. Wintrobe, M.M. (1978). Clinical haematology, London, H. Kimpton Press, p. 448.
  47. Zorriehzahra, M.J., Hassan, M.D., Gholizadeh, M. and Saidi, A.A. (2010). Study of some hematological and biochemical parameters of rainbow trout (Oncorhynchus mykiss) fry in western part of Mazandaran province, Iran Iranian Journal of Fisheries Sciences. 9(1): 185-198.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)