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Research Article

volume 53 issue 3 (march 2019) : 332-335,   Doi: 10.18805/ijar.B-3514

Effect of supplementation of L. plantarum and L. casei based probiotic milk powder on hematology, blood biochemistry and lipid profile of Charles Foster rats

P
Prity Singh
R
Rajendra Kumar Pandey
V
Vinod Kumar Paswan*
S
Satya Prakash Yadav
B
Basant Kumar Bhinchhar
C
Chandra Shekhar Singh
1Department of Animal Husbandry and Dairying, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221 005, Uttar Pradesh, India.
Cite article:- Singh Prity, Pandey Kumar Rajendra, Paswan* Kumar Vinod, Yadav Prakash Satya, Bhinchhar Kumar Basant, Singh Shekhar Chandra (2018). Effect of supplementation of L. plantarum and L. casei based probiotic milk powder on hematology, blood biochemistry and lipid profile of Charles Foster rats. Indian Journal of Animal Research. 53(3): 332-335. doi: 10.18805/ijar.B-3514.

ABSTRACT

Effects of supplementation of L. plantarum and L. casei based spray dried probiotic milk powder on hematology, blood biochemistry and lipid profile was investigated in Charles Foster (CF) rats. Forty-eight male CF rats (21 day old) were divided into four groups and fed on basal diets supplemented with plane spray dried milk powder without any probiotic (control) and L. plantarum, L. casei and the mixed culture of L. plantarum and L. casei based spray dried probiotic milk powder in LP, LC and LPLC treatment groups, respectively. Supplementation of diets with spray dried probiotic milk powder containing either the monoculture of L. plantarum or L. casei or their mixed culture improved health performance of rats in terms of hematological and blood biochemical parameters. Significant decrease in the total serum cholesterol, LDL-cholesterol and triglycerides, and a significant increase in HDL-cholesterol concentration was observed in rats fed on diets supplemented with spray dried probiotic milk powder. Although the improvement in blood biochemical, hematological parameters and serum lipid profile of experimental rats was observed in all the three probiotic milk powder supplemented groups viz. LP, LC and LPLC; however these improvement was more in the L. plantarum and L. casei mixed culture supplemented group as compared to other two monoculture probiotic milk powder supplemented groups. Nevertheless, these increases were only of statistical significance without any clinical relevance as most of these parameters were within the normal physiological values for rats.

REFERENCES

  1. AOAC (2000). Official Methods of Analysis of AOAC International, (17th ed.), Gaithersburg, MD, USA: AOAC.
  2. FAO/WHO. (2001). Joint FAO/WHO Expert Committee on Food Additives Meeting. Safety evaluation of certain mycotoxins in food (No. 74). Food & Agriculture Org.
  3. Haberer, P., Du Toit, M., Dicks, L.M.T., Ahrens, F. and Holzapfel, W.H. (2003). Effect of potentially probiotic lactobacilli on faecal enzyme activity in minipigs on a high-fat, high-cholesterol diet—a preliminary in vivo trial. International Journal of Food Microbiology, 87:287–291.
  4. Hyde, J.,S. and Thomas, D.D. (1973). New EPR methods for the study of very slow motion application to spin-labeled hemoglobin. Ann N Y Academic Science, 222:680–692.
  5. Isolauri, E., Rautanen, T., Juntunen, M., Sillanaukee, P. and Koivula, T. (1991). A human Lactobacillus strain (Lactobacillus casei sp strain GG) promotes recovery from acute diarrhea in children. Pediatrics, 88(1), 90-97.
  6. Jeun, J., Kim, S., Cho, S.Y., Jun, H.J., Park, H.J., Seo, J.G., Chung, M.J. and Lee, S. J. (2010). Hypocholesterolemic effects of Lactobacillus plantarum KCTC3928 by increased bile acid excretion in C57BL/6 mice. Nutrition, 26(3), 321-330.
  7. Kaila, M., Isolauri, E., Soppi, E., Virtanen, E., Laine, S. and Arvilommi, H. (1992). Enhancement of the circulating antibody secreting cell response in human diarrhea by a human lactobacillus strain. Pediatric Research, 32: 141-4.
  8. Kaneko, J.J., Harvey, J.W. and Bruss, M.L. (1997). Clinical Biochemistry of Domestic Animals. 5th ed. Academic Press, San Diego, USA.
  9. Kawase, M., Hashimoto, H., Hosoda, M., Morita, H. and Hosono, A. (2000). Effect of administration of fermented milk containing whey protein concentrate to rats and healthy men on serum lipids and blood pressure. Journal of Dairy Science, 83: 255–263.
  10. McFarland, L.V. (2007). Meta-analysis of probiotics for the prevention of traveler’s diarrhoea. Travel Med Infect Dis, 5(2):97–105.
  11. Ngongang, E.F.T., Tiencheu, B., Achidi, A.U., Fossi, B.T., Shiynyuy, D.M., Womeni, H.M., and François, Z.N. (2016). Effects of probiotic bacteria from yogurt on enzyme and serum cholesterol levels of experimentally induced hyperlipidemic Wistar Albino rats. Am. J. Biol. Life Sci, 4, 48.
  12. NRC, (1995): Nutrient requirements of laboratory rat. National Academic of Science, Fourth Revised Edition. The National Academies Press. Washington. DC. pp. 11-79.
  13. Paswan, V.K. (2012.). Studies on detoxification and nutritive potential of karanj (Pongamia glabra) cake. Ph. D. thesis submitted to Indian Veterinary Research Institute, Izatnagar, Bareilly U.P, India.
  14. Perdigon, G., Nader de Macias, M.E., Alvarez, S., Oliver, G. and Pesce de Ruiz Holgado, A.A. (1990). Prevention of gastrointestinal infection using immunobiological methods with milk fermented with Lactobacillus casei and Lactobacillus acidophilus. J Dairy Res, 57(2):255–264.
  15. Pouwels, P.H., Leer, R.J. and Boersma, W.J.A. (1996). The potential of Lactobacillus as a carrier for oral immunization: Development and preliminary characterization of vector systems for targeted delivery of antigens. Journal of Biotechnology, 44: 183-192
  16. Rowland, I.R., Capurso, L., Collins, K., Cummings, J., Delzenne, N., Goulet, O., Guarner, F., Marteau, P. and Meier, R. (2010). Current level of consensus on probiotic science. Gut Microbes, 1(6):436-439.
  17. Singh,P., Pandey, R.K., Paswan, V.K., Yadav, S.P., Bhinchhar, B.K., and Singh, C.S. (2017). Effect of supplementation of L. plantarum and L. casei based probiotic milk powder on growth performances and nutrient utilization of Charles Foster rats. Indian Journal of Animal Research (In Press).
  18. Snedecor, G.W. and Cochran, W.G. (1989). Stastical Methods, 8th Edition, the Iowa state University press, Ames, Iowa.
  19. WHO, (2009). “Cardiovascular Disease,” Fact sheet 317, WHO, Geneva, Switzerland http://www.who.int/media centre /factsheets/    fs317/en/print.html.
  20. Yurong, Y., Ruiping, S., ShiMin, Z., and Yibao, J. (2005). Effect of probiotics on intestinal mucosal immunity and ultrastructure of cecal tonsils of chickens. Archives of animal nutrition, 59(4), 237-246.
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    Research Article

    volume 53 issue 3 (march 2019) : 332-335,   Doi: 10.18805/ijar.B-3514

    Effect of supplementation of L. plantarum and L. casei based probiotic milk powder on hematology, blood biochemistry and lipid profile of Charles Foster rats

    P
    Prity Singh
    R
    Rajendra Kumar Pandey
    V
    Vinod Kumar Paswan*
    S
    Satya Prakash Yadav
    B
    Basant Kumar Bhinchhar
    C
    Chandra Shekhar Singh
    1Department of Animal Husbandry and Dairying, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221 005, Uttar Pradesh, India.
    Cite article:- Singh Prity, Pandey Kumar Rajendra, Paswan* Kumar Vinod, Yadav Prakash Satya, Bhinchhar Kumar Basant, Singh Shekhar Chandra (2018). Effect of supplementation of L. plantarum and L. casei based probiotic milk powder on hematology, blood biochemistry and lipid profile of Charles Foster rats. Indian Journal of Animal Research. 53(3): 332-335. doi: 10.18805/ijar.B-3514.

    ABSTRACT

    Effects of supplementation of L. plantarum and L. casei based spray dried probiotic milk powder on hematology, blood biochemistry and lipid profile was investigated in Charles Foster (CF) rats. Forty-eight male CF rats (21 day old) were divided into four groups and fed on basal diets supplemented with plane spray dried milk powder without any probiotic (control) and L. plantarum, L. casei and the mixed culture of L. plantarum and L. casei based spray dried probiotic milk powder in LP, LC and LPLC treatment groups, respectively. Supplementation of diets with spray dried probiotic milk powder containing either the monoculture of L. plantarum or L. casei or their mixed culture improved health performance of rats in terms of hematological and blood biochemical parameters. Significant decrease in the total serum cholesterol, LDL-cholesterol and triglycerides, and a significant increase in HDL-cholesterol concentration was observed in rats fed on diets supplemented with spray dried probiotic milk powder. Although the improvement in blood biochemical, hematological parameters and serum lipid profile of experimental rats was observed in all the three probiotic milk powder supplemented groups viz. LP, LC and LPLC; however these improvement was more in the L. plantarum and L. casei mixed culture supplemented group as compared to other two monoculture probiotic milk powder supplemented groups. Nevertheless, these increases were only of statistical significance without any clinical relevance as most of these parameters were within the normal physiological values for rats.

    REFERENCES

    1. AOAC (2000). Official Methods of Analysis of AOAC International, (17th ed.), Gaithersburg, MD, USA: AOAC.
    2. FAO/WHO. (2001). Joint FAO/WHO Expert Committee on Food Additives Meeting. Safety evaluation of certain mycotoxins in food (No. 74). Food & Agriculture Org.
    3. Haberer, P., Du Toit, M., Dicks, L.M.T., Ahrens, F. and Holzapfel, W.H. (2003). Effect of potentially probiotic lactobacilli on faecal enzyme activity in minipigs on a high-fat, high-cholesterol diet—a preliminary in vivo trial. International Journal of Food Microbiology, 87:287–291.
    4. Hyde, J.,S. and Thomas, D.D. (1973). New EPR methods for the study of very slow motion application to spin-labeled hemoglobin. Ann N Y Academic Science, 222:680–692.
    5. Isolauri, E., Rautanen, T., Juntunen, M., Sillanaukee, P. and Koivula, T. (1991). A human Lactobacillus strain (Lactobacillus casei sp strain GG) promotes recovery from acute diarrhea in children. Pediatrics, 88(1), 90-97.
    6. Jeun, J., Kim, S., Cho, S.Y., Jun, H.J., Park, H.J., Seo, J.G., Chung, M.J. and Lee, S. J. (2010). Hypocholesterolemic effects of Lactobacillus plantarum KCTC3928 by increased bile acid excretion in C57BL/6 mice. Nutrition, 26(3), 321-330.
    7. Kaila, M., Isolauri, E., Soppi, E., Virtanen, E., Laine, S. and Arvilommi, H. (1992). Enhancement of the circulating antibody secreting cell response in human diarrhea by a human lactobacillus strain. Pediatric Research, 32: 141-4.
    8. Kaneko, J.J., Harvey, J.W. and Bruss, M.L. (1997). Clinical Biochemistry of Domestic Animals. 5th ed. Academic Press, San Diego, USA.
    9. Kawase, M., Hashimoto, H., Hosoda, M., Morita, H. and Hosono, A. (2000). Effect of administration of fermented milk containing whey protein concentrate to rats and healthy men on serum lipids and blood pressure. Journal of Dairy Science, 83: 255–263.
    10. McFarland, L.V. (2007). Meta-analysis of probiotics for the prevention of traveler’s diarrhoea. Travel Med Infect Dis, 5(2):97–105.
    11. Ngongang, E.F.T., Tiencheu, B., Achidi, A.U., Fossi, B.T., Shiynyuy, D.M., Womeni, H.M., and François, Z.N. (2016). Effects of probiotic bacteria from yogurt on enzyme and serum cholesterol levels of experimentally induced hyperlipidemic Wistar Albino rats. Am. J. Biol. Life Sci, 4, 48.
    12. NRC, (1995): Nutrient requirements of laboratory rat. National Academic of Science, Fourth Revised Edition. The National Academies Press. Washington. DC. pp. 11-79.
    13. Paswan, V.K. (2012.). Studies on detoxification and nutritive potential of karanj (Pongamia glabra) cake. Ph. D. thesis submitted to Indian Veterinary Research Institute, Izatnagar, Bareilly U.P, India.
    14. Perdigon, G., Nader de Macias, M.E., Alvarez, S., Oliver, G. and Pesce de Ruiz Holgado, A.A. (1990). Prevention of gastrointestinal infection using immunobiological methods with milk fermented with Lactobacillus casei and Lactobacillus acidophilus. J Dairy Res, 57(2):255–264.
    15. Pouwels, P.H., Leer, R.J. and Boersma, W.J.A. (1996). The potential of Lactobacillus as a carrier for oral immunization: Development and preliminary characterization of vector systems for targeted delivery of antigens. Journal of Biotechnology, 44: 183-192
    16. Rowland, I.R., Capurso, L., Collins, K., Cummings, J., Delzenne, N., Goulet, O., Guarner, F., Marteau, P. and Meier, R. (2010). Current level of consensus on probiotic science. Gut Microbes, 1(6):436-439.
    17. Singh,P., Pandey, R.K., Paswan, V.K., Yadav, S.P., Bhinchhar, B.K., and Singh, C.S. (2017). Effect of supplementation of L. plantarum and L. casei based probiotic milk powder on growth performances and nutrient utilization of Charles Foster rats. Indian Journal of Animal Research (In Press).
    18. Snedecor, G.W. and Cochran, W.G. (1989). Stastical Methods, 8th Edition, the Iowa state University press, Ames, Iowa.
    19. WHO, (2009). “Cardiovascular Disease,” Fact sheet 317, WHO, Geneva, Switzerland http://www.who.int/media centre /factsheets/    fs317/en/print.html.
    20. Yurong, Y., Ruiping, S., ShiMin, Z., and Yibao, J. (2005). Effect of probiotics on intestinal mucosal immunity and ultrastructure of cecal tonsils of chickens. Archives of animal nutrition, 59(4), 237-246.
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