Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 53 issue 3 (march 2019) : 404-407

Comparative study on biological material for molecular diagnosis of Gallid herpesvirus 2

Venkataramireddy Balena, M.R Reddy, Rajendra Singh
1Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar-243 122, Uttar Pradesh, India
Cite article:- Balena Venkataramireddy, Reddy M.R, Singh Rajendra (2018). Comparative study on biological material for molecular diagnosis of Gallid herpesvirus 2. Indian Journal of Animal Research. 53(3): 404-407. doi: 10.18805/ijar.B-3499.

ABSTRACT

The present study was intended to investigate the sensitivity of different tissues of tumorous and non-tumorous birds to the polymerize chain reaction-baseddiagnosis of Marek’s disease. Tissue samples were collected from spleen, sciatic nerve, feather follicles and tumour parts (if any)of chickens during the routine necropsy examination. The presence of Marek’s disease virus was examined using primers for 132 bp repeats of BamHI-H regionand positive samples were sequenced for 132 bp repeats and Meq gene. In the birds showing tumours, various samples such as the spleen, feather pulp, sciatic nerve and tumour tissue yielded positive results in  92.31%, 88.46%, 100% and 100% cases respectively. Amongst non-tumorous birds, 11.53% spleen, 23.07% feather pulp and 11.53% sciatic nerve were found positive. This is the first comparative study between nerve and other organs samples for molecular diagnosis of Marek’s disease. Data generated shows that the probability of false negative in tumorous birds and false positive in non-tumorous birds was comparatively low while employing nerve tissue than spleen and feather follicles. Thus, it may be concluded from our study that nerves are a more reliable and robust diagnostic organ in comparison to the other organs used for conventional diagnosis.

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