Occurrence of beta-lactamase producing Escherichia coli in healthy and diarrhoeic dogs in Andhra Pradesh, India

DOI: 10.18805/ijar.B-3495    | Article Id: B-3495 | Page : 250-254
Citation :- Occurrence of beta-lactamase producing Escherichia coli in healthy and diarrhoeic dogs in Andhra Pradesh, India.Indian Journal Of Animal Research.2019.(53):250-254
N. Mohammad Sharif, B. Sreedevi, R.K. Chaitanya and Ch. Srilatha sharifnoorbasha@gmail.com
Address : Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University (SVVU), Tirupati-517 502, Andhra Pradesh, India.
Submitted Date : 11-09-2017
Accepted Date : 12-02-2018

Abstract

The present study was carried out to characterize beta-lactam resistance in Escherichia coli isolated from healthy and diarrhoeic dogs. A total of 93 E. coli were isolated from the rectal swabs of 136 dogs (60/92 of healthy dogs and 33/44 of diarrhoeic dogs). Predominant serotypes detected include rough (19 isolates), O141 (5), O9 (2), O126 (2), O128 (2), O15, O20, O35, O49, O63, O85, O101, O116, O117, O118, O119 (1 isolate each) and the rest of 52 isolates were untypable (UT). Disc diffusion method revealed resistance to cefotaxime (41.9%), ceftriaxone (34.4%), ceftazidime (30.1%) and aztreonam (18.2%). Overall frequency of extended spectrum beta-lactamase (ESBL) phenotype was found to be 29% (27/93). Beta-lactamase genes detected include blaAmpC (86.0%), blaSHV (30.1%), blaCTX-M group-1 (19.3%), blaTEM (17.2%), blaOXA (13.9%) and blaCTX-M group-2 (7.5%). The study revealed resistance to commonly prescribed beta-lactams, with ESBL phenotype in E. coli of canine origin in Andhra Pradesh, India. 

Keywords

Beta-lactam resistance Beta-lactamase Dogs Escherichia coli ESBL

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