Molecular characterization of A1/A2 Beta-casein Alleles in Vrindavani crossbred and Sahiwal cattle

DOI: 10.18805/ijar.B-3493    | Article Id: B-3493 | Page : 151-155
Citation :- Molecular characterization of A1/A2 Beta-casein Alleles in Vrindavani crossbred and Sahiwal cattle.Indian Journal Of Animal Research.2019.(53):151-155
Sushil Kumar, Ran Vir Singh and Anuj Chauhan vetsushil09@yahoo.com
Address : Animal Genetics Division, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, Uttar Pradesh, India.
Submitted Date : 6-09-2017
Accepted Date : 2-11-2017

Abstract

In the present investigation Vrindavani cross bred and indigenous Sahiwal cows were genotyped for A1/ A2 b-casein alleles. Present genotypic study on A1/A2 Beta-casein variants was conducted in a population of 660 cattle which comprises of 354 Vrindavani and 306 Sahiwal cows. Out of them 354 Vrindavani cows were maintained at Cattle and Buffalo Farm, IVRI, Izatnagar and 306 Sahiwal cows under study were maintained at Kamdhenu Gaushala, Noormahal, Jalandhar. In Vrindavani cows, all three type of genotype were observed viz., A1A1, A1A2 and A2A2. Genotypic frequencies of A1A1, A1A2 and A2A2 genotypes were 0.11, 0.47 and 0.42 respectively. The frequency of A1 and A2 allelic frequency in this population was 0.35 and 0.65 respectively in Vrindavani cows. In Sahiwal cows, only two types of genotypes were observed viz., A1A2 and A2A2. Genotype frequencies for A1A2 and A2A2 genotypes were 0.13 and 0.87 in Sahiwal cattle. The allelic frequencies for A1 and A2 were 0.06 and 0.94 respectively in Sahiwal cows. The results revealed that maximum genotypic frequency observed was A1A2 (0.47) followed by A2A2 (0.42) and A1A1 (0.11) in Vrindavani cattle while in Sahiwal cattle maximum genotypic frequency of A2A2 (0.87) followed by A1A2 (0.13) respectively. The observed heterozygosity (0.47) and PIC value (0.35) pointed towards the existence of medium genetic variability in the tested population.

Keywords

A1 A2 PIC Sahiwal Vrindavani V%

References

  1. Bech, A.M. and Kristiansen, K.R. (1990). Milk protein polymorphism in Danish dairy cattle and the influence of genetic variants on milk yield. J. Dairy Res. 57: 53–62.
  2. Boltstein, D. R.L., White, M., Skolnik, R.W. and Davis. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphism. Amer J Hum Genet. 32:314-331.
  3. Brantl, V., Teschemacher H., Henschen, A. and Lottspeich F. (1979). Novel opioid peptides derived from casein (b-casomorphins). I. Isolation from bovine casein peptone. Hoppe Seylers Z Physiol Chem 360: 1211–1216.
  4. Crow, J.F. and Kimura, M. (1970). An Introduction to Population Genetics Theory. Burgess Publishing, Minneapolis
  5. Ehrmann, S., Bartenschlager, H. and Geldermann, H. (1997). Quantification of gene effects on single milk proteins in selected groups of dairy cows. J. Anim. Breed. Genet. 114:121-132.
  6. Eigel, W. N., Butler, J. E., Ernstrom, C. A., Farrell, H. M., Halwarker, V. R., Jenness, R. and Whitney, R. M. (1984). Nomenclature of proteins of cow’s milk: fifth revision. J. Dairy Sci., 67: 1599-1631
  7. Elliot, R.B., Harris, D.P., HilL, J.P., Bibby, N.J. and Wasmuth, H.E. (1999). Type I (insulin dependent) diabetes mellitus and cow milk: casein variant consumption. Diabetologia, 42: 292-296
  8. Farrell, H.M., Jimenez-Flores,J.R., Bleck, G.T., Brown, E.M., Butler, J.E. and Creamer, L.K. (2004). Nomenclature of the proteins of cow’s milk-sixth revision. J. Dairy Sci., 87: 1641–1674
  9. Ganguly, I., Gaur, G.K., Umesh, S., Sunil, K. and Sandeep, M. (2013a). b-casein (CSN2) polymorphism in Ongole (Indian Zebu) and (HF x Sahiwal crossbred) cattle. Indian Journal of Biotechnology, 12: 195-198.
  10. Ganguly, I., Sushil, K., Gaur, G.K., Singh, U., Arun, K., Sandeep, M. and Arjava, S. (2013b). Status of b-casein (CSN2) Polymorphism in Frieswal (HF X Sahiwal crossbred) Cattle. International Journal of Biotechnology and Bioengineering Research, 4(3):249-256.
  11. Gill, H.S., Doull, F., Rutherfurd, KJ. and Cross, ML. (2000). Immunoregulatory peptides in bovine milk. Br J Nutr 84: 111–117.
  12. Hanusova, E., Huba, J., Oravcova, M., Polak, P. and Vrtkova, I. (2010).Genetic variants of b-casein in Holstein Dairy Cattle in Slovakia. Slovak. J.Anim. Sci., 43(2): 63-66.
  13. Ikonen, T., Ojala, M. and Ruottinen, O.(1997). Effects of beta and kappa-casein genotypes on first lactation milk production traits in Finnish Ayrshire cows. In: Milk protein polymorphism. Int Dairy Fed: 47–53. 
  14. Kaminski, S., Cieslinska, A. and Kostyra, E.(2007). Polymorphism of bovine â-casein and its potential effect on human health. Journal of Applicational Genetics. 48: 189-198.
  15. Kamiñski, S., Ruoeæ, A. and Cieoeliñska, A. (2006).A note on frequency of A1 and A2 variants of bovine beta-casein locus in Polish Holstein bulls. J Anim Feed Sci 15: 195–198.
  16. Lien, S., Alestrom, P., Hubgland, H. and Rogne, S. (1992). Detection of multiple beta-casein (CASB) alleles by amplification created restriction sites (ACRS). Anim. Genet., 23: 333–38
  17. Laugesen,M. and Elliott,R. (2003). Ischaemic heart disease, type 1 diabetes, and cow milk A1 beta-casein. New. Zeal. Med. J, 116, 1–19.
  18. Malarmathi, M., Senthil Kumar, T M A., Parthiban, M., Muthuramalingam,T. and Palanisammi, A. (2014). Analysis of â- casein gene of A1 and A2 genotype using allele specific PCR in Kangeyam and HF crossbred cattle in Tamil Nadu, Indian J Vet Anim Sci Res , 43:310-315
  19. Manga, I., Ríha, J. and Dvorák, J. (2006). Comparison of influence markers CSN3 and CSN2 on milk performance traits in czech spotted and holstein cattle tested at first, fifth and higher lactation, Acta fytotech. et zootech., 9:13-15
  20. Martien, A.M., Groenen, J. and Van Der Poel, J. (1994). Regulation of expression of milk protein genes: a review. Livest. Prod. Sci., 38: 61–78
  21. Mclachlan, C.N.S. (2001). Beta-casein ischemic heart diseases mortality and other illnesses. Med. Hypotheses., 56: 262-272
  22. Meisel, H. and Bockelmann, W. (1999). Bioactive peptides encrypted in milk proteins: proteolytic activation and thropho-functional properties. Antonie Van Leeuwenhoek 76: 207–215.
  23. Miller, M.J.S., Witherly, S.A. and Clark, D.A. (1990). Casein: a milk protein with diverse biologic consequences. Proc. Soc. Exp. Biol. Med., 195: 143–159
  24. Mishra, B.P., Manishi, M., Prakash, B., Monika, S., Kapila, R., Kishore, A., Kataria, R.R., et al. (2009): Status of milk protein, b-    casein variants among Indian milch animals. Indian Journal of Animal Sciences, 79 (7): 722-725
  25. Muhammed, E.M. and Stephen, M. (2012). b-casein A1A2 Polymorphism and Milk Yield in Vechur, Kasargode Dwarf and Crossbred cattle. Journal of Indian Association, Kerala.,10(3): 5-9
  26. Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proc Nat Acad. Sci. USA 70: 3321–3323
  27. Niki, R., Kim, G.Y., Kimura, T., Takahashi, K., Koyama, K. and Nishinari, K. (1994). Physical properties and microstructure of rennet gels from casein micelles of different sizes. Milchwissenschaft, 49: 325–329
  28. Ostersen, S., Foldaber, J. and Hermansen, J.E. (1997). Effects of stage of lactation, milk protein genotype and body condition at calving on protein composition and renneting properties of bovine milk. J.Dairy Res., 64: 207–219
  29. Haq MRU, Kapila R, Shandilya UK, Dang AK and Kapila S. (2012).Detection of A1 and A2 genetic variants of beta-casein in Indian crossbred cattle by PCR-ACRS.” Milchwissenschaft- Milk Science International. 67.4: 396-398
  30. Roginsky, H. (2003). Encyclopedia of Dairy Sciences. Academic Press, London
  31. Sambrook, J. and Russell, D. (2001). Molecular Cloning. A Laboratory Manual 3rd edition. Cold Spring Harbor Press,Cold Spring Harbour ,N,Y. Sodhi, M., Mukesh, M., Mishra, B.P., Kishore, A., Prakash, B., Kapila, R. and Mclachlan, C.N.S. (2001). CNS â-casein A1, ischaemic heart disease mortality, and other illnesses. Medical Hypoth 56: 262–272
  32. Stewart, A.F., Bonsing, J., Beattie, C.W., Shah, F., Willis, I.M. and Ackinley, A.G. (1987). Complete nucleotide sequences of bovine ás2and â- casein cDNAs: comparison with related sequences in other species. Molecular Biology and Evolution, 4: 231–241
  33. Sun, Z., Zhang, Z., Wang, X., Cade, R., Elmer, Z. and Fregly, M. (2003). Relation of beta-casomorphin to apnea in sudden infant death syndrome. Peptides 24: 937–943
  34. Swaisgood, H. E. (1992). Chemistry of the casein. In: Fox, P.F.(Ed.) Advanced dairy chemistry –I: proteins, . Fox PF, Elsevier, London: 63-77
  35. Tailford, K. A., Berry, C. L., Thomas, A. C. And Campbell, J. H. (2003). A casein variant in cow’s milk is atherogenic. Therosclerosis 170: 13–19
  36. Winkelman, A.M. and Wickham, B.W. (1997): Associations between milk protein genetic variants and production traits in New Zealand dairy cattle. In: Milk protein polymorphism. Proceedings of the IDF Seminar held in Palmerston North. New Zealand. International Dairy Fedding, 38-46. 

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