Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 53 issue 2 (february 2019) : 275-277

Cryptosporidium oocyst shedding in buffalo calves in Haryana: A case study

Krutanjali Swain, Abhilash Routray, Saraswat Sahoo, Subha Ganguly
1Department of Veterinary Parasitology, Faculty of Veterinary Science and Animal Husbandry, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, India.
Cite article:- Swain Krutanjali, Routray Abhilash, Sahoo Saraswat, Ganguly Subha (2018). Cryptosporidium oocyst shedding in buffalo calves in Haryana: A case study. Indian Journal of Animal Research. 53(2): 275-277. doi: 10.18805/ijar.B-3487.

ABSTRACT

Bovine cryptosporidiosisis primarily associated with neonatal diarrhoea with higher morbidity than mortality in young calves till they attain immunological maturity. The present investigation relates to a report on the shedding of Cryptosporidium oocyst in two buffalo calves of buffalo farm, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar from 1st days up to 3 month of age at 15 days interval using simple conventional microscopy. By using formol-ether concentration technique followed by modified Ziehl-Neelsen (ZN) acid fast staining, Cryptosporidium oocysts were concentrated and identified. The Cryptosporidium oocysts appeared as reddish pink coloured bodies against a bluish/greenish coloured background at oil immersion using ZN staining kit. The maximum oocyst shedding was observed (2.3 oocyst / field) during 16 to 30 days of age. The oocyst shedding gradually decreased with increase in age afterwards clearly indicating the disease of young buffalo calves.

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